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Peculiarities of Phagocytosis of Opsonized and Nonopsonized Bacteria S. Aureus and E. Coli by Human Neutrophil Granulocytes Studied by Atomic Force Microscopy

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Abstract—

Differences in the phagocytosis process of opsonized and nonopsonized strains of Staphylococcus aureus 2879 M and Escherichia coli 321 were studied. Differences in the character of pseudopodia during phagocytosis by neutrophil granulocytes (NGs) of opsonized and nonopsonized bacteria were detected, and differences in the nature of pseudopodia in reactions to gram-positive and gram-negative microorganisms were not detected. For the first time in dynamic observations at the late stages of phagocytosis, changes in the volume of nuclei and their movement, variations in the intersegment distance of the nuclei, and a slight increase in the volume of NGs were shown. A decrease in the rigidity of the membrane–cytoskeleton NG complex correlating with the intensity of phagocytosis and opsonization of bacteria was shown for the first time. It was established that opsonization does not affect the oxygen-dependent metabolism of NGs and, at the same time, introduces significant adjustments in the implementation of oxygen-independent bactericidal mechanisms of cells.

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ACKNOWLEDGMENTS

This study was supported by the Russian Science Foundation, project no. 16-14-10179.

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Authors and Affiliations

  1. Scientific and Educational Center for Physics of Solid-State Nanostructures, Lobachevsky State University of Nizhny Novgorod, 603950, Nizhny Novgorod, Russia

    S. N. Pleskova, R. N. Kriukov, E. V. Razumkova, S. Yu. Zubkov & N. V. Abarbanel

  2. Nanotechnology and Biotechnology Department, Alekseev Nizhny Novgorod State Technical University, 603950, Nizhny Novgorod, Russia

    S. N. Pleskova, E. V. Razumkova & N. V. Abarbanel

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  1. S. N. Pleskova
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  2. R. N. Kriukov
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Correspondence to S. N. Pleskova.

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Statement on the welfare of animals. This article does not contain any studies with animals performed by any of the authors.

Statement of compliance with standards of research involving humans as subjects. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. Blood sampling was performed in the morning, after the donors signed the consent form.

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Translated by V. Mittova

Abbreviations: AFM—atomic force microscopy, ROSs—reactive oxygen species, ACP—acid phosphatase, LCPs—lysosomal cationic proteins, LCT—lysosomal cation test, MPO—myeloperoxidase, NGs—neutrophil granulocytes, NBT—nitroblue tetrazolium, ACC—average cytochemical coefficient, AP—alkaline phosphatase, PAMPs—pathogen-associated molecular patterns, PBS—phosphate buffered saline, PGRP—peptidoglycan recognition protein.

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Pleskova, S.N., Kriukov, R.N., Razumkova, E.V. et al. Peculiarities of Phagocytosis of Opsonized and Nonopsonized Bacteria S. Aureus and E. Coli by Human Neutrophil Granulocytes Studied by Atomic Force Microscopy. Cell Tiss. Biol. 12, 496–505 (2018). https://doi.org/10.1134/S1990519X18060081

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