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Serological and genomic characterisation of group A rotaviruses from lambs

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Summary

Four lamb rotaviruses were characterised serologically by reactions with monoclonal antibodies and genomically by hybridisation assays and sequencing. Each was found to be distinct. Three viruses belonged to the bovine genogroup and were of subgroup I. These viruses possessed serotypes G3, G6, and G10. Their corresponding P types were P1, P11, and P14 respectively. The only previous isolation of a rotavirus with VP4 of type P14 was also from lambs. The fourth isolate was G9P8, which is the first record of a G9 rotavirus from a species other than man.

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References

  1. Browning GF, Chalmers, RM, Fitzgerald TA, Snodgrass DR (1991) Serologic and genomic characterization of L338, a novel equine group A rotavirus G serotype. J Gen Virol 72: 1059–1064

    Google Scholar 

  2. Browning GF, Chalmers RM, Fitzgerald TA, Corley KTT, Campbell I, Snodgrass DR (1992) Rotavirus serotype G3 predominates in horses. J Clin Microbiol 30: 59–62

    Google Scholar 

  3. Charpilienne A, Borras F, D'Auriol L, Galibert F, Cohen J (1986) Sequence of the gene encoding the outer glycoprotein of the bovine rotavirus (RF strain) and comparison with homologous genes from four bovine, simian and human rotaviruses. Ann Inst Pasteur 137: 71–77

    Google Scholar 

  4. Chasey D, Banks J (1984) The commonest rotaviruses from neonatal lamb diarrhoea in England and Wales have atypical electropherotypes. Vet Rec 115: 326–327

    Google Scholar 

  5. Clark HF, Hoshino Y, Bell LM, Groff J, Hess G, Bachmann P, Offit PA (1987) Rotavirus isolate WI61 representing a presumptive new human serotype. J Clin Microbiol 25: 1757–1762

    Google Scholar 

  6. Das BK, Gentsch JR, Hoshino Y, Ishida S, Nakagomi O, Bhan MK, Kumar R, Glass RI (1993) Characterization of the G serotype and genogroup of New Delhi newborn rotavirus strain 116E. Virology 197: 99–107

    Google Scholar 

  7. Dunn SJ, Burns JW, Cross TL, Vo PT, Ward RL, Bremont M, Greenberg HB (1994) Comparison of VP4 and VP7 of five murine rotavirus strains. Virology 203: 250–259

    Google Scholar 

  8. Estes MK, Cohen J (1989) Rotavirus gene structure and function. Microbiol Rev 53: 410–449

    Google Scholar 

  9. Fuchs F, Leparc I, Kopecka H, Garin D, Aymard M (1993) Use of cRNA digoxigenin-labelled probes for detection of enteroviruses in the environment. J Virol Methods 42: 217–226

    Google Scholar 

  10. Gorziglia M, Larralde G, Kapikian AZ, Chanock RM (1990) Antigenic relationships among human rotaviruses as determined by outer capsid protein VP4. Proc Natl Acad Sci USA 87: 7155–7159

    Google Scholar 

  11. Herring AJ, Inglis NF, Ojeh CK, Snodgrass DR, Menzies JD (1982) Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels. J Clin Microbiol 16: 473–477

    Google Scholar 

  12. Hussein HA, Parwani AV, Rosen BI, Lucchelli A, Saif LJ (1993) Detection of rotavirus serotypes G1, G2, G3, and G11 in faeces of diarrhoeic calves by using polymerase chain reaction-derived cDNA probes. J Clin Microbiol 31: 2491–2496

    Google Scholar 

  13. Kapikian AZ, Chanock RM (1990) Rotaviruses. In: Fields BN, Knipe DM (eds) Virology, 2nd ed. Raven Press, New York, pp 1353–1404

    Google Scholar 

  14. Larralde G, Flores J (1990) Identification of gene 4 alleles among human rotaviruses by polymerase chain reaction-derived probes. Virology 179: 469–473

    Google Scholar 

  15. Larralde G, Gorziglia MI (1992) Distribution of conserved and specific epitopes on the VP8 subunit of rotavirus VP4. J Virol 66: 7438–7443

    Google Scholar 

  16. Lin M, Imai M, Ikegami N, Bellamy AR, Summers D, Nuss DL, Deibel R, Furuichi Y (1987) cDNA probes of individual genes of human rotavirus distinguish viral subgroups and serotypes. J Virol Methods 15: 285–289

    Google Scholar 

  17. Mackow ER, Barnett JW, Chan H, Greenberg HB (1989) The rhesus rotavirus outer capsid protein VP4 functions as a hemagglutinin and is antigenically conserved when expressed by a baculovirus recombinant. J Virol 63: 1661–1668

    Google Scholar 

  18. Makabe T, Sato M, Ouneda S, Inaba Y (1986) Hemagglutination with ovine rotavirus. Arch Virol 90: 153–158

    Google Scholar 

  19. McNulty MS, Allan GM, Pearson GR, McFerran JB, Curran WL, McCracken RM (1976) Reovirus-like agent (rotavirus) from lambs. Infect Immun 14: 1332–1338

    Google Scholar 

  20. Nakagomi O, Nakagomi T, Akatani K, Ikegami N (1989) Identification of rotavirus genogroups by RNA-RNA hybridization. Mol Cell Probes 3: 251–261

    Google Scholar 

  21. Nakagomi T, Ohshima A, Akatani K, Ikegami N, Katsushima N, Nakagomi O (1990) Isolation and molecular characterization of a serotype 9 human rotavirus strain. Microbiol Immunol 34: 77–82

    Google Scholar 

  22. Nakagomi O, Nakagomi T (1991) Genetic similarity and diversity among mammalian rotaviruses in relation to interspecies transmission of rotavirus. Arch Virol 120: 43–55

    Google Scholar 

  23. Parwani AV, Rosen BI, McCrae MA, Saif LJ (1992) Development of cDNA probes for typing group A bovine rotaviruses on the basis of VP4 specificity. J Clin Microbiol 30: 2717–2721

    Google Scholar 

  24. Parwani AV, Hussein HA, Rosen BI, Lucchelli A, Navarro L, Saif LJ (1993) Characterization of field strains of group A bovine rotaviruses by using polymerase chain reaction-generated G and P Type-specific cDNA probes. J Clin Microbiol 31: 2010–2015

    Google Scholar 

  25. Shen S, Burke B, Desselberger U (1993) Nucleotide sequences of the VP4 and VP7 genes of a Chinese lamb rotavirus: evidence for a new P type in a G10 type virus. Virology 197: 497–500

    Google Scholar 

  26. Snodgrass DR, Herring JA, Gray EW (1976) Experimental rotavirus infection in lambs. J Comp Pathol 86: 637–642

    Google Scholar 

  27. Snodgrass DR, Herring JA, Linklater KA, Dyson DA (1977) A survey of rotaviruses in sheep in Scotland. Vet Rec 100: 341

    Google Scholar 

  28. Snodgrass DR, Fitzgerald TA, Campbell I, Scott FMM, Browning GF, Miller DL, Herring AJ, Greenberg HB (1990) Rotavirus serotypes 6 and 10 predominate in cattle. J Clin Microbiol 28: 504–507

    Google Scholar 

  29. Snodgrass DR, Hoshino Y, Fitzgerald TA, Smith M, Browning GF, Gorziglia M (1992) Identification of four VP4 serological types (P serotypes) of bovine rotaviruses using viral reassortants. J Gen Virol 73: 2319–2325

    Google Scholar 

  30. Steele AD, Garcia D, Sears J, Gerna G, Nakagomi O, Flores J (1993) Distribution of VP4 gene alleles in human rotaviruses by using probes to the hyperdivergent region of the VP4 gene. J Clin Microbiol 31: 1735–1740

    Google Scholar 

  31. Szucs G, Kende M, Uj M (1987) Shift in genomic molecular patterns of human rotaviruses over an eighteen-month period in Hungary. Intervirology 28: 110–113

    Google Scholar 

  32. Urasawa S, Hasegawa A, Urasawa T, Taniguchi K, Wakasugi F, Suzuki H, Inouye S, Pongprot B, Supawadee J, Suprasert S, Rangsiyanond P, Tonusin S, Yamazi Y (1992) Antigenic and genetic analyses of human rotaviruses in Chiang Mai, Thailand: evidence for a close relationship between human and animal rotaviruses. J Infect Dis 166: 227–234

    Google Scholar 

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Author notes

  1. M. Munoz

    Present address: Facultad de Veterinaria, Universidad de Leon, Leon, Spain

Authors and Affiliations

  1. Moredun Research Institute, Edinburgh, Scotland

    T. A. Fitzgerald, M. Munoz, A. R. Wood & D. R. Snodgrass

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  1. T. A. Fitzgerald
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  2. M. Munoz
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  3. A. R. Wood
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  4. D. R. Snodgrass
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Fitzgerald, T.A., Munoz, M., Wood, A.R. et al. Serological and genomic characterisation of group A rotaviruses from lambs. Archives of Virology 140, 1541–1548 (1995). https://doi.org/10.1007/BF01322528

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  • DOI: https://doi.org/10.1007/BF01322528

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