Summary
Virus yield, viral RNA synthesis, viral protein synthesis, cytopathology, and virus-induced shutoff of host protein synthesis were examined in rabbit kidney (RK) cells infected with eastern equine encephalitis (EEE) viruses. The New Jersey (NJ) strain replicated most rapidly, and exhibited a distinct pattern in the synthesis of structural and non-structural viral proteins. The Louisiana (La) strain and the Ets-4 mutant derived from it replicated more slowly than NJ, and synthesized most of their viral proteins simultaneously. The properties of the Ets-4 mutant appeared to be similar in RK cells and chick embryo fibroblasts. Although the viral peptides comprising the viral RNA polymerase could not be conclusively identified, two non-structural proteins of molecular weights 105,000 and 85,000 were temporally associated with increasing rates of polymerase activity in NJ-infected RK cells and were found in increased amounts in Ets-4-in-fected cells compared to La-infected cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Brown, A.: Differences in maximum and minimum plaque-forming temperature among selected group A arboviruses. Virology21, 362–372 (1963).
Ho, M., andM. K. Breining: Conditions for the production of an interferon appearing in chick cell cultures infected with Sindibs virus. J. Immunol.89, 177 to 186 (1962).
Igarashi, A.: Protein synthesis and formation of Chikungunya virus infected BHK-21 cells. Biken J.13, 289–302 (1970).
Ke, Y. H., andM. Ho: Characterization of virus- and endotoxin-induced interferon obtained from the serum and urine of rabbits. J. Virol.1, 883–890 (1967).
Kennedy, S. I. T.: The effect of enzymes on the structural and biological properties of Semliki Forest virus. J. gen. Virol.23, 129–143 (1974).
Lubiniecki, A. S., J. A. Armstrong, andM. Ho: The pattern of virus-specific peptide synthesis in rabbit cells infected with eastern equine encephalitis virus and its response to interferon. Proc. Soc. exp. Biol. (N.Y.)145, 1014–1017 (1974).
Lubiniecki, A. S., J. A. Armstrong, andM. Ho: Eastern equine encephalitis virus: Quantitative study of the effects of interferon on virus replication. Arch. ges. Virusforsch.44, 52–64 (1974).
Morser, M. J., andD. C. Burke: Cleavage of virus-specified polypeptides in cells infected with Semliki Forest virus. J. gen. Virol.22, 395–409 (1974).
Ranki, M., L. Kaariainen, andO. Renkonen: Semliki Forest virus glycoproteins and canavanine. Acta path. microbiol. scand.80 (B), 760–768 (1972).
Schlesinger, S. O., andM. J. Schlesinger: Formation of Sindbis virus proteins: identification of a precursor for one of the envelope proteins. J. Virol.10, 925–932 (1972).
Schlesinger, M. J., S. Schlesinger, andB. W. Burge: Identification of a second glycoprotein Sindbis virus. Virology47, 539–541 (1972).
Stern, R., andR. M. Friedman: Double-stranded RNA synthesized in animal cells in the presence of actinomycin D. Nature (Lond.)226, 612–616 (1970).
Strauss, J. H., B. W. Burge, andJ. E. Darnell: Sindbis virus infection of chick and hamster cells: Synthesis of virus specific proteins. Virology37, 367–376 (1969).
Tan, K. B., andF. Sokol: Virion-bound protein kinase in Semliki Forest and Sindbis viruses. J. Virol.13, 1245–1253 (1974).
Zebovitz, E., andA. Brown: Altered viral ribonucleic acid synthesis by a temperature sensitive mutant of eastern equine encephalitis virus. J. molec. Biol.50, 185–198 (1970).
Author information
Authors and Affiliations
Additional information
With 6 Figures
This work was supported in part by NIH Grant 5 R 22-AI-02686.
Rights and permissions
About this article
Cite this article
Lubiniecki, A.S. Replication of eastern equine encephalitis viruses (New Jersey and Louisiana strains and the Ets-4 mutant) in rabbit kidney cells. Archives of Virology 47, 21–29 (1975). https://doi.org/10.1007/BF01315589
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01315589