Summary
Rabbit convalescent and hyperimmune sera, human patient and blood donor sera, as well as cerebrospinal fluids of humans with herpes simplex virus encephalitis all recognize similar major antigenic components in herpes simplex virus infected rabbit or human cells as shown by electrophoretic analysis of immunoprecipitates. Besides the main glycoproteins with an apparent molecular weight of 100,000 (peak I) the antisera precipitate glycoproteins in a region of an apparent mol. wt. of 60,000–80,000 (peak II), which were resolved into distinct glycoprotein species only by antibody-containing cerebrospinal fluids. The peak II glycoproteins appear on the surface of the infected cell early, and absorb neutralizing antibodies, whereas the peak I glycoproteins are less accessible. Both antigens can be demonstrated in the cell as early as about 2 hours post infection. All major antigenic components studied were found to be glycosylated except one protein with an apparent mol. wt. of 110,000. The herpesvirus specificity of these antigens is demonstrated by a variety of control experiments. The antigens detected are virion components.
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Egan, M. L., Lautenschleger, J. T., Coligan, J. E., Todd, W. C.: Radioimmune assay of carcinoembryonic antigens. Immunochem.9, 289–299 (1972).
Figueroa, M. E., Rawls, W. E.: Biological markers for differentiation of herpesvirus strains of oral and genital origin. J. gen. Virol.4, 259–267 (1969).
Fleissner, E., Tress, E.: Chromatographic and electrophoretic analysis of viral proteins from hamster and chicken cells transformed by Rous Sarcoma Virus. J. Virol.11, 250–262 (1973).
Gerloff, R. K., Hoyer, B. H., McLaren, L. C.: Precipitation of radio-labelled poliovirus with specific antibody and antiglobulin. J. Immunol.89, 550–557 (1962).
Halpern, M. S., Bolognesi, D. P., Friis, R. R., Mason, W. S.: Expression of the major viral glycoprotein of avian tumor virus in cells of chf (+) chicken embryos. J. Virol.15, 1131–1140 (1975).
Hampar, B., Martos, L. M.: Immunological relationships. In:Kaplan, A. S. (ed.), The Herpesviruses, 221–259. New York: Academic Press 1973.
Hayashi, K., Rosenthal, J., Notkins, A. L.: Iodine 125 labeled antibody to viral antigens: Binding to the surface of virus infected cells. Science176, 516–518 (1972).
Honess, R. W., Powell, K. L., Robinson, D. J., Sim, C., Watson, D. H.: Type specific and type common antigens in cells infected with herpes simplex virus type 1 and on the surface of naked and enveloped particles of the virus. J. gen. Virol.22, 159–169 (1974).
Honess, R. W., Watson, D. H.: Herpes simplex virus-specific polypeptides studied by polyacrylamide gel electrophoresis of immune precipitates. J. gen. Virol.22, 171–185 (1974).
Kaplan, A. S., Ben-Porat, T.: Synthesis of proteins in cells infected with herpesvirus, VI. Characterization of the proteins of the viral membrane. Proc. Nat. Acad. Sci. U.S.66, 799–806 (1970).
Kaplan, A. S., Erickson, J. S., Ben-Porat, T.: Synthesis of proteins in cells infected with herpesvirus, X. Proteins excreted by cells infected with herpes simplex virus, types 1 and 2. Virology64, 132–143 (1975).
Kemp, M. C., Perdue, M. L., Rogers, H. W., O'Callaghan, D. J., Randall, C. C.: Structural polypeptides of the hamster strain of equine herpes virus type 1: Products associated with purification. Virology61, 361–375 (1974).
Klenk, H.-D., Caliguiri, L. A., Choppin, P. W.: The proteins of parainfluenza virus SV 5, II. The carbohydrate content and glycoproteins of the virion. Virology42, 473–481 (1970).
Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London)227, 680–684 (1970).
Lausch, R. N., Jones, C., Christie, D., Hay, K. A., Rapp, F.: Spleen cell-mediated cytotoxicity of hamster cells transformed by herpes simplex virus: Evidence for virus-specific membrane antigen. J. Immunol.114, 459–465 (1975).
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin-phenol reagent. J. biol. Chem.193, 255–275 (1951).
Ludwig, H.: Untersuchungen am genetischen Material von Herpesviren. Med. Microbiol. Immunol.157, 186–211 (1972).
Ludwig, H., Becht, H., Rott, R.: Inhibition of herpes virus-induced cell fusion by Concanavalin A, antisera, and 2-deoxy-D-glucose. J. Virol.14, 307–314 (1974).
Ludwig, H., Biswal, N., Benyesh-Melnick, M.: Studies on the relatedness of herpesviruses through DNA-DNA hybridization. Virology49, 95–101 (1972).
Ludwig, H., Rott, R.: Effect of 2-deoxy-D-glucose on herpesvirus-induced inhibition of cellular DNA synthesis. J. Virol.16, 217–221 (1975).
Martin, M. L., Palmer, E. L., Kissling, R. E.: Complement-fixing antigens of herpes simplex virus types 1 and 2: Reactivity of capsid, envelope, and soluble antigens. Infect. Immun.5, 248–254 (1972).
Melnick, J. L., Courtney, R. J., Powell, K. L., Schaffer, P. A., Benyesh-Melnick, M., Dreesman, G. R., Anzai, T., Adam, E.: Studies on herpes simplex virus and cancer. Cancer Res.36, 845–856 (1976).
Nahmias, A. J., Shore, S. L., Kohl, S., Starr, S. E., Ashman, R. B.: Immunology of herpes simplex virus infection: Relevance to herpes simplex virus vaccines and cervical cancer. Cancer Res.36, 836–844 (1976).
Nii, S., Morgan, C., Rose, H. M., Hsu, K. C.: Electron microscopy of herpes simplex virus, IV. Studies with ferritin conjugated antibodies. J. Virol.2, 1172–1184 (1968).
Norrby, E., Link, H., Olson, J. E., Panelius, M., Salmi, A., Vandvik, B.: Comparison of antibodies against different viruses in cerebrospinal fluid and serum samples from patients with multiple sclerosis. Infect. Immun.10, 688–694 (1974).
Piraino, F. F., Sedmak, G., Altshuler, C., Pierce, R.: Rapid antigenic typing of herpesvirus hominis isolates on the surface of infected cells by kinetic binding of125I-labeled HVI antibodies. Amer. J. clin. Pathol.62, 581–590 (1974).
Plummer, G., Goodheart, C. R., Miyagi, M., Skinner, G. R. B., Thouless, M. E., Wildy, P.: Herpes simplex viruses: Discrimination of types and correlation between different characteristics. Virology60, 206–216 (1974).
Ponce de Leon, M., Hessle, H., Cohen, G. H.: Separation of herpes simplex virus-induced antigens by Concanavalin A affinity chromatography. J. Virol.12, 766–774 (1973).
Powell, K. L., Buchan, A., Sim, C., Watson, D. H.: Type-specific protein in herpes simplex virus envelope reacts with neutralizing antibodies. Nature249, 360–361 (1974).
Savage, T., Roizman, B., Heine, J. W.: Immunological specificity of the glycoproteins of herpes simplex virus subtypes 1 and 2. J. gen. Virol.17, 31–48 (1972).
Schneweis, K. E., Nahmias, A. J.: Antigens of herpes simplex virus type 1 and 2-immunodiffusion and inhibition passive hemagglutination studies. Z. Immun. Forsch.141, 471–487 (1971).
Schwartz, J., Elizan, T. S.: Altered immunologic specificity of cells infected with herpes simplex virus: Recognition by human antiserum. Z. Immun. Forsch.148, 291–298 (1975).
Spear, P. G.: Glycoproteins specified by herpes simplex virus type 1: Their synthesis, processing and antigenic relatedness to HSV-2 glycoproteins. In:de Thé, G., Epstein, M. A., zur Hausen, H. (eds.), Oncogenesis and Herpesviruses II, 49 to 61. Lyon: Intern. Agency for Res. on Cancer 1975.
Spear, P. G., Roizman, B.: Proteins specified by herpes simplex virus, V. Purification and structural proteins of the herpesvirion. J. Virol.9, 143–159 (1972).
Sterz, H., Ludwig, H., Rott, R.: Immunologic and genetic relationship between herpes simplex virus and bovine herpes mammillitis virus. Intervirology2, 1–13 (1973/74).
Vestergaard, B. F.: Crossed immunoelectrophoretic characterization of herpesvirus hominis type 1 and 2 antigens. Acta path. microbiol. scand. sect. B.81, 808–810 (1973).
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Pauli, G., Ludwig, H. Immunoprecipitation of herpes simplex virus type 1 antigens with different antisera and human cerebrospinal fluids. Archives of Virology 53, 139–155 (1977). https://doi.org/10.1007/BF01314855
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DOI: https://doi.org/10.1007/BF01314855