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The immunological response of pigs and Guinea pigs to antigens of African swine fever virus

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Summary

Pigs vaccinated with glutaraldehyde-fixed alveolar macrophages (AM) infected with African swine fever virus (ASFV) had an accelerated serological response after subsequent challenge and a slight reduction in levels of viraemia. Vaccination of pigs with detergent-treated infected AM produced no detectable serological response and no protection against homologous challenge.

Guinea pigs were vaccinated with glutaraldehyde-fixed ASFV-infected cells, detergent-treated infected cells, detergent-treated infected spleen homogenate, purified ASFV or sonicated infected cells. Antibody was detectable by ELISA after vaccination with all preparations except the detergent-treated infected spleen vaccine. However, vaccination with purified ASFV or sonicated infected cells induced antibodies that were also strongly reactive in antibody-dependent cell-mediated cytotoxicity and complement-mediated lysis assays. If such antibodies are protective, immunization of pigs with purified ASFV or sonicated infected cells may induce a protective immunity.

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Authors and Affiliations

  1. Animal Virus Research Institute, Pirbright, Surrey, United Kingdom

    A. J. Forman, R. C. Wardley & P. J. Wilkinson

  2. Australian National Animal Health Laboratory, CSIRO Division of Animal Health, P.O. Box 679, 3220, Geelong, Vic., Australia

    A. J. Forman

Authors
  1. A. J. Forman
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  2. R. C. Wardley
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  3. P. J. Wilkinson
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Forman, A.J., Wardley, R.C. & Wilkinson, P.J. The immunological response of pigs and Guinea pigs to antigens of African swine fever virus. Archives of Virology 74, 91–100 (1982). https://doi.org/10.1007/BF01314703

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  • DOI: https://doi.org/10.1007/BF01314703

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