Skip to main content
SpringerLink
Log in

Studies on the neutralizing antibody to herpes simplex virus

I. Effect of heterotypic antigenic stimulus on the type specificity of neutralizing antibody in rabbits

  • Original Papers
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

Effect of heterotypic antigenic stimulus on the antibody response in rabbits previously exposed to only one type of virus was investigated. The complement requiring neutralizing antibody (CRN) was found in the IgM fraction as well as the IgG, but the conventional neutralizing antibody (N) was detected only in the fraction containing IgG. The CRN antibody found in the IgM fraction was most predominantly modified by the inoculation of heterotypic virus, and resulted in a relative increase in CRN antibody titers against the heterotypic virus, whereas antibodies in the IgG fraction, either CRN or N, were slightly affected by the heterotypic stimulus. When the heterotypic virus was given 9 months after the initial inoculation of homotypic virus, the antibody response in the IgM fraction formed the same pattern as seen in the primary response against the type of virus inoculated as the booster. These findings should pave the way for making accurate serological assessments, including the type of virus in present infections, irrespective of involvement of a superinfection with the heterotypic virus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Ankerst, J., Christensen, P., Kjellen, L., Kronvall, G.: A routine diagnostic test for IgA and IgM antibodies to rubella virus: Absorption of IgG withstaphylococcus aureus. J. inf. Dis.130, 268–273 (1974).

    Google Scholar 

  2. Caroline, S., Watson, D. H.: The role of type specific and cross reacting structural antigens in the neutralization of herpes simplex virus types 1 and 2. J. gen. Virol.19, 217–233 (1973).

    Google Scholar 

  3. Dowdle, W. R., Nahmias, A. J., Harwell, R. W., Paul, F. P.: Association of antigenic type of herpesvirus hominis with site of viral recovery. J. Immunol.99, 974–980 (1967).

    Google Scholar 

  4. Hampar, B., Notkins, A. L., Mage, M., Keehn, M. A.: Heterogeneity in the properties of 7S and 19S rabbit neutralizing antibodies to herpes simplex virus. J. Immunol.100, 586–593 (1968).

    Google Scholar 

  5. Hampar, B., Martos, L. M., Chakrabarty, M., Burroughs, M. A. K.: Late 19S rabbit antibody neutralization test for differentiating herpes simplex virus types 1 and 2. J. Immunol.104, 593–598 (1970).

    Google Scholar 

  6. Kawana, T., Shinkai, K., Yoshino, K.: Typing of herpes simplex virus strains of genital and nongenital origins. Japan. J. Microbiol.18, 235–241 (1974)

    Google Scholar 

  7. Nahmias, A. J., Josey, W. E., Naib, Z. M., Luce, C. F., Guest, B. A.: Antibodies to herpesvirus hominis types 1 and 2 in humans. II. Women with cervical cancer. Amer. J. Epidemiol.91, 547–552 (1970).

    Google Scholar 

  8. Ohashi, M., Ozaki, Y.: Application of indirect hemagglutination test to measurement of type specific antibody to herpes simplex virus in human sera. Microbiol. Immunol.23, 801–804 (1979).

    Google Scholar 

  9. Ozaki, Y., Ishiguro, T., Ohashi, M., Sawaragi, I., Ito, Y.: Antibodies to herpesvirus type 1 and type 2 among Japanese cervical cancer patients. Gann69, 119–122 (1978).

    Google Scholar 

  10. Pauls, F. P., Dowdle, W. R.: A serologic study of herpesvirus hominis strains by microneutralization tests. J. Immunol.98, 941–947 (1967).

    Google Scholar 

  11. Plummer, G., Waner, J. L., Bowling, C. P.: Comparative studies of type 1 and type 2 “herpes simplex viruses”. Brit. J. exp. Pathol.49, 202–208 (1968).

    Google Scholar 

  12. Plummer, G.: A review of the identification and titration of antibodies to herpes simplex viruses type 1 and type 2 in human sera. Cancer Res.33, 1469–1476 (1973).

    Google Scholar 

  13. Rawls, W. E., Tompkins, W. A. F., Melnick, J. L.: The association of herpesvirus type 2 and carcinoma of the uterine cervix. Amer. J. Epidemiol.89, 547–554 (1969).

    Google Scholar 

  14. Rawls, W. E., Iwamoto, K., Adam, E., Melnick, J. L.: Measurement of antibodies to herpesvirus types 1 and 2 in human sera. J. Immunol.104, 599–606 (1970).

    Google Scholar 

  15. Royston, I., Aurelian, L.: The association of genital herpesvirus with cervical atypia and carcinomain situ. Amer. J. Epidemiol.91, 531–538 (1970).

    Google Scholar 

  16. Seto, A.: Comparative studies of Aal allotype specificity in IgG and IgM of rabbits. II. Phenotypic expression of allotype specificities in rabbits exposed in neonatal life to antibodies directed against Aal allotypic determinants specific for IgG. J. Biochem.72, 865–878 (1972).

    Google Scholar 

  17. Schmidt, N. J., Forghani, B., Lennette, E. H.: Type specificity of complement requiring and immunoglobulin M neutralizing antibody in initial herpes simplex virus infections of humans. Infect. Immun.12, 728–732 (1975).

    Google Scholar 

  18. Schneweis, K. E.: Serologische Untersuchungen zur Typedifferenzierung des Herpesvirus hominis. Z. Immunitätsforschung124, 24–48 (1962).

    Google Scholar 

  19. Shinkai, K., Yoshino, K.: Complement requirement of neutralizing antibodies in different classes of immunoglobulin appearing in rabbits and guinea pigs after primary and booster immunization with herpes simplex virus. Japan. J. Microbiol.19, 25–34 (1967).

    Google Scholar 

  20. Smith, J. W.,Glorioso, J. C.: Effect of cross-immunization on monotypic antibody responses to herpes simplex virus types 1 and 2. J. Immunol.116, 898–903 (1976).

    Google Scholar 

  21. Yoshino, K., Taniguchi, S.: The appearance of complement requiring neutralizing antibodies by immunization and infection with herpes simplex virus. Virology22, 193–201 (1964).

    Google Scholar 

  22. Yoshino, K., Taniguchi, S.: Evaluation of the demonstration of complement-requiring neutralizing antibody as a means for early diagnosis of herpes virus infections. J. Immunol.96, 196–203 (1966).

    Google Scholar 

  23. Yoshino, K., Hashimoto, M., Shinkai, K.: Studies on the neutralization of herpes simplex virus. VIII. Significance of viral sensitization for inactivation by complement. Mircobiol. Immunol.21, 231–241 (1977).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Microbiology, Shiga University of Medical Science, Shiga, Japan

    M. Ohashi & Y. Ozaki

Authors
  1. M. Ohashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Y. Ozaki
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

With 5 Figures

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ohashi, M., Ozaki, Y. Studies on the neutralizing antibody to herpes simplex virus. Archives of Virology 67, 57–69 (1981). https://doi.org/10.1007/BF01314602

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01314602

Keywords

Search

Navigation