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The rapid concentration and purification of influenza virus from allantoic fluid

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Summary

Influenza virus was quantitatively recovered from infectious allantoic fluid by precipitation with 8 per cent (wt/vol) polyethylene glycol 6000 (PEG-6000). Virus concentrated by PEG-6000 treatment was rapidly purified by gel permeation chromatography through controlled pore glass beads. The purity of virus preparations achieved by this method, when judged by three independent criteria, was comparable to virus preparations purified by conventional density gradient procedures.

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Authors and Affiliations

  1. Virology Division, Bureau of Laboratories, Center for Disease Control, Public Health Service, U.S. Department of Health, Education, and Welfare, Viral Exanthems Branch, Atlanta, Georgia, USA

    J. T. Heyward, R. A. Klimas, Marianne D. Stapp & J. F. Obijeski

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  1. J. T. Heyward
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  2. R. A. Klimas
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  3. Marianne D. Stapp
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  4. J. F. Obijeski
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Heyward, J.T., Klimas, R.A., Stapp, M.D. et al. The rapid concentration and purification of influenza virus from allantoic fluid. Archives of Virology 55, 107–119 (1977). https://doi.org/10.1007/BF01314484

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  • DOI: https://doi.org/10.1007/BF01314484

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