Abstract
Salinity effects on ultrastructure, size and density of chloride cells in the larvae of ayuPlecoglossus altivelis (Temminck and Schlegel) were examined. Ayu larvae hatched in freshwater were reared in seawater and freshwater at the Fisheries Laboratory, University of Tokyo, and the Institute of Zoology, Academia Sinica, between 1984 and 1986. Freshwater-hatched larvae modify the intercellular organization and the junctional structure of skin chloride-cells within 1 h after direct transfer to 30‰ seawater. The gills of seawater-reared larvae develop chloride cells earlier than those of freshwaterreared larvae. Following the development, a more rapid decrease in number of skin chloride-cells occurs in the freshwater group, while a more significant increase in size of skin chloride-cells occurs in the seawater group. These results indicate that chloride cells possibly play a critical role in osmoregulation in the early life stages of ayu as in the adult, and that the skin chloride-cells may perform this function until the development of the gill chloridecells is complete.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature cited
Alderdice, D. F. (1988). Osmotic and ionic regulation in teleost eggs and larvae. In: Hoar, W. S., Randall, D. J. (eds.) Fish physiology, vol. 11 A. Academic Press, San Diego, p. 163–251
Claude, P. (1978). Morphological factors influencing transepithelial permeability: a model for the resistance of the zonulae occludentes. J. Membrane Biol. 39: 219–232
Claude, P., Goodenough, A. (1973). Fracture faces of zonulae occludentes from “tight” and “leaky” epithelia. J. Cell Biol. 58: 390–400
Depeche, J. (1973). Ultrastructure of the yolks and pericardial sac surface in the embryo of teleostPoecilia reticulata. Z. Zellforsch. 141: 236–253
Foskett, J. K., Bern, H. A., Machen, T. E., Conner, M. (1983). Chloride cells and the hormonal control of teleost fish osmoregulation. J. exp. Biol. 106: 255–281
Foskett, J. K., Scheffey, C. (1982). The chloride cell: definitive identification as the salt secretory cell in teleost. Science, N.Y. 215: 164–166
Holliday, F. G. T. (1965). Osmoregulation in marine teleost eggs and larvae. Calif. coop. ocean. Fish. Invest. Rep. 10: 89–95
Hwang, P. P. (1987). Tolerance and ultrastructural responses of branchial chloride cells to salinity changes in the euryhaline teleostOreochromis mossambicus. Mar. Biol. 94: 643–649
Hwang, P. P. (1988). Ultrastructural study on the multicellular complex of chloride cells in teleosts. Bull. Inst. Zool., Acad. Sin. 27: 225–233
Hwang, P. P. (1989). Distribution of chloride cells in teleost larvae. J. Morphol. 200: 1–8
Hwang, P. P. (1990). Immunocytochemical identification of prolactin cells in the pituitary gland of tilapia larva (Oreochromis mossambicus; Teleostei). Cell Tissue Res. 260: 203–205
Hwang, P. P., Hirano, R. (1985). Effects of environmental salinity on intercellular organization and junctional structure of chloride cells in early stages of teleost development. J. exp. Zool. 236: 115–126
Hwang, P. P., Sun, C. M. (1989). Putative role of adenohypophysis in the osmoregulation of tilapia larvae (Oreochromis mossambicus; Teleostei): An ultrastructure study. Gen. comp. Endocr. 73: 335–341
Hwang, P. P., Sun, C. M., Wu, S. M. (1989). Changes of plasma osmolality, chloride concentration and gill Na-K-ATPase activity in tilapiaOreochromis mossambicus during seawater acclimation. Mar. Biol. 100: 295–299
Jones, M. P., Holliday, F. G. T., Dunn, A. E. G. (1966). The ultrastructure of the epidermis of larvae of the herring (Clupea harengus) in relation to the rearing salinity. J. mar. biol. Ass. U.K. 46: 235–239
Karnaky, K. J., Degnan, K. J., Zadunaisky, J. A. (1977). Chloride transport across isolated opercular epithelium of killifish: A membrane rich in chloride cells. Science, N.Y. 195: 203–205
Kashiwagi, M., Sato, R. (1969). Studies on the osmoregulation of the chum salmonOncorhynchus keta (Walbaum). II. Histological observations of the branchial epithelium according to the growth of the fish from hatching. Tohoku J. agric. Res. 20: 212–221
Keys, A., Willmer, E. N. (1932). “Chloride secretion cells” in the gills of fish, with special reference to the common eel. J. Physiol. 76: 368–378
Lasker, R., Threadgold, L. T. (1968). “Chloride cells” in the skin of the larval sardine. Exp. Cell Res. 52: 582–590
Leatherland, J. F., Lin, L. (1975). Activity of the pituitary gland in embryo and larval stages of coho salmonOncorhynchus kisutch. Can. J. Zool. 53: 297–310
Payan, P., Girard, J. P., Mayer-Gostan, N. (1984). Branchial ion movements in teleosts: the roles of respiratory and chloride cells. In: Hoar, W. S., Randall, D. J. (eds.) Fish physiology, vol. 10 B. Academic Press, Orlando, p. 39–63
Sardet, C., Pisam, M., Maetz, J. (1979). The surface epithelium of teleostean fish gills. J. Cell Biol. 80: 96–117
Sargent, J. R., Bell, M. V., Kelly, K. F. (1980). The nature and properties of sodium ion plus potassium ion-activated adenosine triphosphotase and its role in marine salt secreting epithelia. In: Lahlou, B. (ed.) Epithelial transport in the lower vertebrates. Cambridge University Press, London, p. 251–267
Shelbourne, J. E. (1957). Site of chloride regulation in marine fish larvae. Nature, Lond. 108: 920–922
Shen, A. C. Y., Leatherland, J. F. (1978). Structure of the yolk-sac epithelium and gills in the early development stages of rainbow trout (Salmo gairdneri) maintained in different ambient salinities. Envir. Biol. Fish 3: 345–354
Silva, P., Solomon, R., Spokes, K., Epstein, F. H. (1977). Ouabain inhibition of gill Na-K-ATPase: relationship to active chloride transport. J. exp. Zool. 199: 419–426
Thomson, A. J., Sargent, J. R. (1977). Changes in the levels of chloride cells and (Na+ + K+)-dependent ATPase in the gills of yellow and silver eels adapting to seawater. J. exp. Zool. 200: 33–40
Utida, S., Kamiya, M., Shirai, N. (1971). Relationship between the activity of Na-K-activated adenosinetriphosphatase and the number of chloride cells in eel gills with special reference to sea-water adaptation. Comp. Biochem. Physiol. 38A:443–448
Vicker, T. (1961). A Study of the so-called ‘chloride-secretory’ cells of the gill of teleosts. Q. Jl. microsc. Sci. 104: 507–518
Weisbart, M. (1968). Osmotic and ionic regulation in embryos, alevin and fry of the five species of Pacific salmon. Can. J. Zool. 163: 237–264
Author information
Authors and Affiliations
Additional information
Communicated by M. Anraku, Tokyo
Rights and permissions
About this article
Cite this article
Hwang, P.P. Salinity effects on development of chloride cells in the larvae of ayu (Plecoglossus altivelis). Mar. Biol. 107, 1–7 (1990). https://doi.org/10.1007/BF01313236
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01313236