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The pathogenesis of rat virus infection in infant and juvenile rats after oronasal inoculation

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Summary

The pathogenesis of rat virus (RV) infection was studied in random-bred Sprague-Dawley rats after oronasal inoculation of a recent RV isolate designated RV-Yale (RV-Y). RV-Y was pathogenic for rats inoculated as infants (2 days) whereas rats inoculated as juveniles (4 weeks) had asymptomatic infection and no lesions. Rats inoculated as infants developed pantropic infection accompanied by hepatic necrosis, granuloprival cerebellar hypoplasia and hemorrhagic encephalopathy. Virological and serological studies showed that virus could persist in inoculated rats for at least 35 days and for at least 28 days after seroconversion was first detected. Immuno-histochemical results indicated that RV-Y infects tissues conducive to virus excretion including kidney and lung. RV-Y also was found in genital tissues of some rats. Athymic juvenile rats inoculated intraperitoneally with RV-Y had a poor humoral immune response and harbored infectious virus for at least 3 weeks, whereas infection in euthymic control rats was detected for 1 week. These studies indicate that RV-Y can persists in the presence of humoral immunity and suggest that transmission of infection could occur for a substantial period after seroconversion. They also suggest that immunodeficient rats have increased susceptibility to persistent infection.

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References

  1. Bass R, Hetrick FM (1978) Persistent infection of a human lymphocyte cell line (Molt-4) with the Kilham rat virus. J Infect Dis 137: 210–212

    Google Scholar 

  2. Campbell DA, Staal SP, Manders EK, Bonnard GD, Oldham RK, Salzman LA, Herberman RB (1977) Inhibition of in vitro lymphoproliferative responses by in vivo passaged rat 13762 mammary adenocarcinoma cells. II. Evidence that Kilham rat virus is responsible for the inhibitory effect. Cell Immunol 33: 378–391

    Google Scholar 

  3. Cole GA, Nathanson N, Rivet H (1970) Viral hemorrhagic encephalopathy of rats. II. Pathogenesis of central nervous system lesions. Am J Epidemiol 91: 339–350

    Google Scholar 

  4. Coleman GL, Jacoby RO, Bhatt PN, Smith AL, Jonas AM (1983) Naturally occurring lethal parvovirus infection of juvenile and young rats. Vet Pathol 20: 49–56

    Google Scholar 

  5. El Dadah AN, Nathanson N, Smith KO, Squire RA, Santos G, Melby EC (1967) Viral hemorrhagic encephalopathy of rats. Science 156: 392–394

    Google Scholar 

  6. Hsu SM, Raine L, Fanger H (1981) Use of avidin-biotin-peroxidase complex (ABC) immunoperoxidase technique: a comparison between ABC and unlabeled antibody (PAP procedures). J Histochem Cytochem 29: 577–580

    Google Scholar 

  7. Jacoby RO, Bhatt PN, Gaertner DE, Paturzo FX, Smith AL (1987) Duration of transmission of rat virus infection. Lab Anim Sci (in press).

  8. Kilham L (1966) Viruses of laboratory and wild rats. Natl Cancer Inst Monogr 20: 117–135

    Google Scholar 

  9. Kilham L, Ferm VH (1961) Rat virus (RV) infections of pregnant, fetal, and newborn rats. Proc Soc Exp Biol Med 106: 825–829

    Google Scholar 

  10. Kilham L, Margolis G (1966) Spontaneous hepatitis and cerebellar hypoplasia in suckling rats due to congenital infection with rat virus. Am J Pathol 49: 457–475

    Google Scholar 

  11. Kilham L, Margolis G (1969) Transplacental infection of rats and hamsters induced by oral and parenteral inoculations of H-1 and rat viruses (RV). Teratology 2: 111–123

    Google Scholar 

  12. Kilham L, Margolis G (1974) Transmission of rat virus in milk of rats. J Infect Dis 129: 737–740

    Google Scholar 

  13. Kilham L, Margolis G (1975) Problems of human concern arising from animal models of intrauterine and neonatal infections due to viruses: a review. I. Introduction and virologic studies. Prog Med Virol 20: 113–143

    Google Scholar 

  14. Kilham L, Olivier L (1959) A latent virus of rats isolated in tissue culture. Virology 7: 428–437

    Google Scholar 

  15. Lipton H, Nathanson N, Hodous J (1977) Enteric transmission of parvoviruses: pathogenesis of rat virus infection in adult rats. Am J Epidemiol 96: 443–446

    Google Scholar 

  16. Lum GS (1970) In vitro studies of rat viruses. I. Effects of long-term culture. Oncology 24: 401–415

    Google Scholar 

  17. Margolis G, Kilham L (1970) Parvovirus infections, vascular endothelium, and hemorrhagic encephalopathy. Lab Invest 22: 478–488

    Google Scholar 

  18. Margolis G, Kilham L (1972) Rat virus infection of megakaryocytes: a factor in hemorrhagic encephalopathy. Exp Mol Pathol 16: 326–340

    Google Scholar 

  19. Margolis G, Kilham L, Johnson RH (1971) The parvoviruses and replicating cells: Insights into the pathogenesis of cerebellar hypoplasia. Prog Neuropathol 1: 168–201

    Google Scholar 

  20. Margolis G, Kilham L, Ruffolo PR (1968) Rat virus disease as an experimental model of neonatal hepatitis. Exp Mol Pathol 8: 1–20

    Google Scholar 

  21. McLean IW, Makane PK (1974) Periodate-lysine-paraformaldehyde fixative. A new fixative for immunoelectron microscopy. J Histochem Cytochem 22: 1077–1083

    Google Scholar 

  22. Nottay BK, Kew OM, Hatch MH, Heyward JT, Obijeski JF (1981) Molecular variation of type 1 vaccine-related and wild polioviruses during replication in humans. Virology 108: 405–423

    Google Scholar 

  23. Novotny JF, Hetrick FM (1970) Pathogenesis and transmission of Kilham rat virus infection in rats. Infect Immun 2: 298–303

    Google Scholar 

  24. Parrish CR, O'Connell PH, Evermann JF, Carmichael LE (1985) Natural variation of canine parvoviruses. Science 230: 1046–1048

    Google Scholar 

  25. Paturzo FX, Jacoby RO, Bhatt PN, Smith AL, Gaertner DJ, Ardito RB (1987) Persistence of rat virus in seropositive rats as detected by explant culture. Arch Virol 95: 137–142

    Google Scholar 

  26. Phillips TL, Leong GF (1967) Kidney cell proliferation after unilateral nephrectomy as related to age. Cancer Res 27: 286–292

    Google Scholar 

  27. Porter DD, Cho HJ (1980) Aleutian disease of mink: a model for persistent infection. In: Fraenkel-Conrat H, Wagner RR (eds) Comprehensive virology, vol 16. Plenum Press, New York, pp 233–256

    Google Scholar 

  28. Reed LJ, Muench H (1938) A simple method of estimating fifty percent endpoints. Am J Hyg 27: 493–497

    Google Scholar 

  29. Robey RE, Woodman DR, Hetrick FM (1968) Studies on the natural infection of rats with the Kilham rat virus. Am J Epidemiol 88: 139–143

    Google Scholar 

  30. Smith AL (1983) Response of weanling random-bred mice to inoculation with minute virus of mice. Lab Anim Sci 33: 37–39

    Google Scholar 

  31. Smith AL (1985) An enzyme immunoassay for identification and quantification of infectious murine parvovirus in cultured cells. Virol Meth 11: 321–327

    Google Scholar 

  32. Steiner JW, Perz ZM, Taichman LB (1966) Cell population dynamics in the liver. A review of quantitative morphological techniques applied to the study of physiological and pathological growth. Exp Mol Pathol 5: 146–181

    Google Scholar 

  33. Tattersall P (1978) Susceptibility to minute virus of mcie as a function of host cell differentiation. In: Ward DC, Tattersall P (eds) Replication of mammalian parvoviruses. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 53–72

    Google Scholar 

  34. Tattersall P, Bratton J (1983) Reciprocal productive and restrictive virus-cell interactions of immunosuppressive and prototype strains of minute virus of mice. J Virol 46: 944–955

    Google Scholar 

  35. Tattersall P, Cotmore S. F. (1986) The rodent parvoviruses. In: Bhatt PN, Jacoby RO, Morse HC, New AE (eds) Viral and mycoplasmal infectious of laboratory rodents: effect on biomedical research. Academic Press, New York pp 305–348

    Google Scholar 

  36. Wozniak J, Hetrick F (1969) Persistent infection of a rat nephroma cell line with Kilham rat virus. J Virol 4: 313–314

    Google Scholar 

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Authors and Affiliations

  1. Section of Comparative Medicine, Yale University School of Medicine, New Haven, Connecticut, U.S.A.

    R. O. Jacoby, P. N. Bhatt, Diane J. Gaertner, Abigail L. Smith & Elizabeth A. Johnson

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  1. R. O. Jacoby
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  2. P. N. Bhatt
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  3. Diane J. Gaertner
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  4. Abigail L. Smith
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  5. Elizabeth A. Johnson
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Jacoby, R.O., Bhatt, P.N., Gaertner, D.J. et al. The pathogenesis of rat virus infection in infant and juvenile rats after oronasal inoculation. Archives of Virology 95, 251–270 (1987). https://doi.org/10.1007/BF01310784

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  • DOI: https://doi.org/10.1007/BF01310784

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