Abstract
No reports have described the catabolic mechanism of substance Pin vivo. We studied the effects of hepatic or renal transit on substance P, vasoactive intestinal polypeptide, and gastrin in anesthetized dogs. It was found that the liver plays a more important role in vasoactive intestinal polypeptide catabolism than the kidney and the kidney is more important in gastrin catabolism than the liver. Substance P was more rapidly degraded than the other two peptides in both organs. The transrenal substance P loss measured byC-terminal antiserum differed from that measured byN-terminal antiserum, although there was no difference in the liver. This suggested that there were different patterns of cleavage of substance P between the liver and the kidney, and that itsC terminal was degraded more strongly than itsN terminal in the kidney.
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References
von Euler US, Gaddum JH: An unidentified depressor substance in certain tissue extracts. J Physiol 72:74–87, 1931
Iversen SD: Behavioural effects of substance P through dopaminergic pathways in the brain.In Substance P in the Nervous System. R Porter, M O'Connor (eds). London, Pitman Books Ltd, 1982, pp 307–324
Cooper PE, Fernstrom MH, Rorstsd OP, Leeman SE, Martin JB: The regional distribution of somatostatin, substance P and neurotensin in human brain. Brain Res 218:219–232, 1981
Pearse AGE, Polak JM: Immunocytochemical localization of substance P in mammalian intestine. Histochemistry 41:373–375, 1975
Schultzberg M: Peripheral nervous system.In Chemical Neuroanatomy. PC Empson (ed). New York, Raven Press, 1983, pp 1–52
Minagawa H, Shiosaka S, Inoue H, Hayashi N, Kasahara A, Kamata T, Tohyama M, Shiotani Y: Origins and three dimensional distribution of substance P-containing structures on the rat stomach using whole-mount tissue. Gastroenterology 86:51–59, 1984
Hallberg D, Pernow B: Effects of substance P on various vascular beds in the dog. Acta Physiol Scand 93:277–285, 1975
Rosell S, Bjorkroth U, Chang D, Yamaguchi I, Wan YP, Rackur G, Fisfer G, Folkers K: Effects of substance P and analogs on isolated guinea pig ileum.In Substance P. US von Euler B Pernow (eds). New York, Raven Press, 1977, pp 83–88
Mills IH, Macfarlane NAA, Ward PE: Increase in kallikrein excretion during the natriuresis produced by atrical infusion of substance P. Nature 247:108–109, 1974
Walling MW, Brastius TA, Kimberg DV: Effects of calcitonin and substance P on the transport of Ca, Na, and Cl across rat ileumin vitro. Gastroenterology 73:89–94, 1977
Fahrenkrug J: Vasoactive intestinal polypeptide: Measurement, distribution and putative neurotransmitter function. Digestion 27:149–169, 1979
Chayvialle JA, Miyata M, Rayford L, Thompson JC: Vasoactive intestinal polypeptide in portal, right heart, and aortic plasma in dogs. Scand J Gastroenterol 13:39, 1978
Kabemura T, Misawa T, Chijiiwa Y, Nasu T: The liver and vasoactive intestinal polypeptide catabolism. Med Sci Res 16:333–334, 1988
Davidson WD, Springberg PD, Newell R, Falkinburg NR: Renal extraction and excretion of endogenous gastrin in the dog. Gastroenterology 64:955–961, 1973
Stephenson SL, Kenny AJ: Metabolism of neuropeptides. Hydolysis of the angiotensins, bradykinin, substance P and oxytoscin by pig kidney microvillar membranes. Biochem J 241:237–247, 1987
Krause JE: Metabolism and biological actions.In Substance P CC Jordan, P Oehme (eds). London, Talor and Francis, 1985, pp 13–31
Chijiiwa Y, Misawa T, Ibayashi H: Evidence of local mechanism involvement in vasoactive intestinal polypeptide release from canine small intestine. Gastroenterology 90:1877–1881, 1986
Iinuma K, Ikeda I, Takai M, Yanagawa Y, Kurata K: Gastrin immunoassay with polyethyleneglycol method. Radioisotopes 31:350–356, 1982
Piga M, Altieri P, Floris A: Vasoactive intestinal polypeptide (VIP) plasma levels in chronic renal failure. J Nucl Med Allied Sci 28:77–80, 1984
Keltz TN, Straus E, Yalow RS: Degradation of vasoactive intestinal polypeptide by tissue homogenates. Biochem Biophys Res Commun 92:669–674, 1980
Thompson JC, Rayford PL, Ramus NI: Patterns of release and uptake of heterogenous forms of gastrin.In Gastrointestinal Hormones, JC Thompson (ed). Austin, University of Texas Press, 1977, pp 125–151
Dencker H, Hakanson R, Liedberg G, Norryd C, Oscarson J, Rehfeld JF, Stadil F: Gastrin in portal and peripheral venous blood after feeding in man. Gut 14:856–860, 1973
Movsas B, Mannor GE, Yalow RS: Degradation of the 34 amino acid gastrin by rat tissue homogenates. Life Sci 36:89–95, 1985
Reeder DD, Jackson BM, Brandt EN, Jr, Thompson JC: Rate and pattern of disappearance of exogenous gastrin in dogs. Am J Physiol 222:1571–1574, 1972
Villar HV, Reeder DD, Rayford PL, Thompson JC: Rate of disappearance of circulating endogenous gastrin in dogs. Surgery 81:404–408, 1977
Chayvialle JA, Rayford PL, Thompson JC: Radioimmunoassay study of hepatic clearance and disappearance half-time of somatostatin and vasoactive intestinal polypeptide in dogs. Gut 22:732–737, 1981
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Kabemura, T., Misawa, T., Chijiiwa, Y. et al. Substance P, vasoactive intestinal polypeptide, and gastrin catabolism in canine liver and kidney. Digest Dis Sci 37, 1661–1665 (1992). https://doi.org/10.1007/BF01299855
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DOI: https://doi.org/10.1007/BF01299855