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Higher-plant plasma membrane cytochromeb 561: A protein in search of a function

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Summary

During the past twenty years evidence has accumulated on the presence of a specific high-potential, ascorbate-reducibleb-type cytochrome in the plasma membrane (PM) of higher plants. This cytochrome is named cytochromeb 561 (cytb 561) according to the wavelength maximum of its α-band in the reduced form. More recent evidence suggests that this protein is homologous to ab-type cytochrome present in chromaffin granules of animal cells. The plant and animal cytochromes share a number of strikingly similar features, including the high redox potential, the ascorbate reducibility, and most importantly the capacity to transport electrons across the membrane they are located in. The PM cytb 561 is found in all plant species and in a variety of tissues tested so far. It thus appears to be a ubiquitous electron transport component of the PM. The cytochromesb 561 probably constitute a novel class of transmembrane electron transport proteins present in a large variety of eukaryotic cells. Of particular interest is the recent discovery of a number of plant genes that show striking homologies to the genes coding for the mammalian cytochromesb 561. A number of highly relevant structural features, including hydrophobic domains, heme ligation sites, and possible ascorbate and monodehydroascorbate binding sites are almost perfectly conserved in all these proteins. At the same time the plant gene products show interesting differences related to their specific location at the PM, such as potentially N-linked glycosylation sites. It is also clear that at least in several plants cytb 561 is represented by a multigene family. The current paper presents the first overview focusing exclusively on the plant PM cytb 561, compares it to the animal cytb 561, and discusses the possible physiological function of these proteins in plants.

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Abbreviations

Asc:

ascorbate

cyt:

cytochrome

DHA:

dehydroascorbate

E0′:

standard redox potential

EST:

expressed sequence tag

His:

histidine

MDA:

monodehydroascorbate

Met:

methionine

PM:

plasma membrane

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  1. Department of Biology, University of Antwerp, Groenenborgerlaan 171, 2020, Antwerp, Belgium

    Han Asard, Jyoti Kapila & Wim Verelst

  2. Institute of Biophysics, Biological Research Center, Hungarian Academy of Sciences, Szeged

    Alajos Bérczi

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  1. Han Asard
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Asard, H., Kapila, J., Verelst, W. et al. Higher-plant plasma membrane cytochromeb 561: A protein in search of a function. Protoplasma 217, 77–93 (2001). https://doi.org/10.1007/BF01289417

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