Summary
Internodal cells ofChara, grown in culture either at pH 5.7, 6.5 or 7.5, were studied to determine their chloride influx capability, the quantitative aspects of charasome morphology and the degree to which these two parameters could be correlated. In cells grown at pH 5.7 the charasomes were relatively small, were widely spaced on the plasma membrane, and contributed only a 0.6% increase to the surface area of the plasma membrane in the acid region of the cell. In contrast, the charasome membrane surface area of cells grown at pH 7.5 had increased × 19, the density of charasomes on the cell surface increased × 42, thus producing a × 3.57 increase in the acid region plasma membrane surface area. Chloride influx in cells grown at pH 7.5 was × 8.7–12.7 greater than in cells grown at pH 5.7. Cells that had been starved of chloride exhibited a × 2.4 average increase in the rate of chloride influx. Our observations establish the existence of a positive correlation between the rate of chloride influx and the increase in membrane surface area due to charasomes, although other factors, such as the effect of pH on transport-related enzymes, and the effect of charasome structure on chemical equilibria, may also be of importance.
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References
Arnon, D. I., 1949: Copper enzymes in isolated chloroplasts. Polyphenoloxidase inBeta vulgaris. Plant Physiology24, 1–15.
Barton, R., 1965: An unusual organelle in the peripheral cytoplasm ofChara cells. Nature205, 201.
Beilby, M. J., Walker, N. A., 1981: Chloride transport inChara: I. Kinetics and current voltage curves for probable proton symport. J. exp. Bot.32, 43–54.
Crawley, J. A. C., 1965: A cytoplasmic organelle in association with the cell walls ofChara andNitella cells. Nature205, 200–201.
Franceschi, V. R., 1981: Membrane structure-function relationships in several characean species. Ph. D. Dissertation, University of California, Davis, U.S.A.
Franceschi, V. R., Lucas, W. J., 1980: Structure and possible function(s) of charasomes; complex plasmalemma-cell wall elaborations present in some characean species. Protoplasma104, 253–271.
— —, 1981 a: The glycosome ofChara: Ultrastructure, development, and composition. J. Ultrastruct. Res.75, 218–228.
— —, 1981 b: The charasome periplasmic space. Protoplasma107, 269–284.
— —, 1982: The relationship of the charasome to chloride uptake inChara corallina: physiological and histochemical investigations. Planta154, 525–537.
Keifer, D. W., Franceschi, V. R., Lucas, W. J., 1982: Plasmalemma chloride transport inChara corallina: inhibition by 4,4′Diisothiocyano-2,2′-disulfonic acid stilbene. Plant Physiol.70, 1327–1334.
Lucas, W. J., 1983: Photosynthetic assimilation of exogenous HCO −3 by aquatic plants. Ann. Rev. Plant Physiol.34, 71–104.
—,Franceschi, V. R., 1981: Characean charasome-complex and plasmalemma vesicle development. Protoplasma107, 255–267.
Österlind, S., 1951: Inorganic carbon sources of green algae. IV. Photoactivation of some factor necessary for bicarbonate assimilation. Physiol. Plant.4, 514–527.
Pesacreta, T. C., Lucas, W. J., 1984: Plasma membrane coat and a coated vesicle-associated reticulum of membranes: their structure and possible interrelationship inChara corallina. J. Cell Biol.98, 1537–1545.
Price, G. D.,Badger, M. R.,Bassett, M. E.,Whitecross, M. I., 1985: Involvement of plasmalemmasomes and carbonic anhydrase in photosynthetic utilization of bicarbonate inChara corallina. Aust. J. Plant Physiol., in press.
—,Whitecross, M. I., 1983: Cytochemical localization of ATPase activity on the plasmalemma ofChara corallina. Protoplasma116, 65–74.
Sanders, D., 1980a: Control of Cl− influx inChara by cytoplasmic Cl− concentration. J. Membrane Biol.52, 51–60.
—, 1980b: The mechanism of Cl− transport at the plasma membrane ofChara corallina. I. Cotransport with H+. J. Membrane Biol.53, 129–141.
—,Hansen, U.-P., 1981: Mechanism of Cl− transport at the plasma membrane ofChara corallina. II. Transinhibition and the determination of H+/Cl− binding order for a reaction kinetic model. J. Membrane Biol.58, 139–153.
Smith, F. A., 1970: The mechanism of chloride transport in characean cells. New Phytol.69, 903–917.
—,Walker, N. A., 1976: Chloride transport inChara corallina and the electrochemical potential difference for hydrogen ions. J. exp. Bot.27, 451–459.
Spear, D. G., Barr, J. K., Barr, C. E., 1969: Localization of hydrogen ion and chloride ion fluxes inNitella. J. gen. Physiol.54, 397–414.
Spurr, A. R., 1969: A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res.26, 31–43.
Steelmann Nielsen, E., 1960: Uptake of CO2 by the plant. In: Encyclopedia of Plant Physiology, Vol. V, The Assimilation of Carbon Dioxide (Ruhland, W., ed.), pp. 70–84. BerlinGöttingen-Heidelberg: Springer.
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Lucas, W.J., Keifer, D.W. & Pesacreta, T.C. Influence of culture medium pH on charasome development and chloride transport inChara corallina . Protoplasma 130, 5–11 (1986). https://doi.org/10.1007/BF01283326
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DOI: https://doi.org/10.1007/BF01283326