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Electrical fusion of protoplasts from sycamore mutants and hybrid selection on hypoxanthine-aminopterin-thymidine (HAT) medium

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Summary

An electrical fusion method has been used to form somatic hybrids between protoplasts of two mutant cell lines of sycamore tissue culture cells. Both mutants will not grow in a hypoxanthine-aminopterin-thymidine (HAT) medium. It was possible to select the fused hybrids from homospecific fusion products and nonfused protoplasts by the use of HAT medium. In this way the viability and regeneration of the fused cells during the first few weeks of culture could be evaluated. An electron microscopic examination of the fusion process showed that it occurred at a series of points along the surface of the plasmalemma. Cytoplasmic bridges between the two cells were formed separated by vesicles which later dispersed to give complete cytoplasmic continuity between the cells.

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Author notes

  1. Nadine Burlion

    Present address: Laboratory of Photobiology, Department of Botany B22, University of Liege, Sort Tilman, B-4000, Liege, Belgium

Authors and Affiliations

  1. Department of Biochemistry, University of Cambridge, Tennis Court Road, CB2 1QW, Cambridge, UK

    Nadine Burlion & D. H. Northcote

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  1. Nadine Burlion
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  2. D. H. Northcote
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Burlion, N., Northcote, D.H. Electrical fusion of protoplasts from sycamore mutants and hybrid selection on hypoxanthine-aminopterin-thymidine (HAT) medium. Protoplasma 138, 23–31 (1987). https://doi.org/10.1007/BF01281181

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  • DOI: https://doi.org/10.1007/BF01281181

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