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Ultrastructural study of different types of callus from leaf expiants ofAesculus hippocastanum L.

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Summary

The cell ultrastructure in three types of callus obtained from leaf explants ofAesculus hippocastanum L. has been studied. Remarkable differences have been shown between the cells of the forerunner E1 callus and those of the callus arising from it, according to the culture conditions.

The peculiar characteristics of E1 are the scarcity of intercellular spaces and the occurrence of autophagic vacuoles in the cells.

An embryogenic friable callus (E2) is formed in time when E1 is maintained on solid culture medium. The E2 cells show cytological features typical of a higher metabolic level and contain starch. Diffused middle lamella digestion leads to the detachment of small embryogenic cell aggregates consisting of vacuolated parenchymatous-like cells and small meristematic cells which may be regarded as embryoids initials.

Shaking E1 in the same liquid medium and subsequent culture on solid medium lead to the differentiation of a non-embryogenic callus (NE), whose cells are very large and highly vacuolated, devoid of starch and with organelle-rich cytoplasm. The NE callus shows a high degree of growth, but does not attain embryogenic competence in time.

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Abbreviations

c :

cell

cr :

crystal

cw :

cell wall

d :

dictyosome

er :

endoplasmic reticulum

m :

mitochondrion

mb :

microbody

n :

nucleus

p :

plastid

s :

starch

v :

vacuole

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Authors and Affiliations

  1. Istituto Botanico “Hanbury” ed Orto Botanico dell'Università, Corso Dogali 1 C, I-16136, Genova, Italy

    Paola Profumo & Paola Gastaldo

  2. Dipartimento di Biologia, Università di Padova, Italy

    Nicoletta Rascio

Authors
  1. Paola Profumo
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  2. Paola Gastaldo
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  3. Nicoletta Rascio
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Profumo, P., Gastaldo, P. & Rascio, N. Ultrastructural study of different types of callus from leaf expiants ofAesculus hippocastanum L.. Protoplasma 138, 89–97 (1987). https://doi.org/10.1007/BF01281017

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  • DOI: https://doi.org/10.1007/BF01281017

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