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Pea seed storage proteins — Immunocytochemical localization with protein a-gold by electron microscopy

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Summary

The pea seed storage proteins legumin and vicilin have been localized by electron microscopy using a post-embedding immunocytochemical double-labelling technique. Highly purified antibodies from sheep, specific to legumin and vicilin, were sequentially reacted on sections of pea cotyledon tissue embedded in glycol methacrylate or Spurr's epoxy resin. The sheep antibodies were visualized indirectly by reaction with Staphylococcal Protein A labelled with colloidal gold. Two discrete sizes of colloidal gold particles can be used for simultaneous localization of two antigens. In near mature tissue, the storage proteins are restricted to organelles, called protein bodies. Individual protein bodies contain both legumin and vicilin.

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Authors and Affiliations

  1. Division of Plant Industry, CSIRO, P.O. Box 1600, 2601, Canberra City, A.C.T., Australia

    Stuart Craig & Adele Millerd

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  1. Stuart Craig
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  2. Adele Millerd
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Craig, S., Millerd, A. Pea seed storage proteins — Immunocytochemical localization with protein a-gold by electron microscopy. Protoplasma 105, 333–339 (1981). https://doi.org/10.1007/BF01279230

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  • DOI: https://doi.org/10.1007/BF01279230

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