Skip to main content
SpringerLink
Log in

Cytochemical and ultrastructural studies on normal and segregated nucleoli in meristematic cells

  • Published:
Protoplasma Aims and scope Submit manuscript

Summary

The location of the two nucleolar components, in meristematic root tip cells ofAllium cepa has been studied. The “segregation” of granular and fibrous components of the nucleoli was induced by adenosine-3′deoxyriboside. The relationship between the ultrastructure and the stain affinities of both nucleolar regions has been determined by using different selective nucleolar stains.

The “pars fibrosa” appears positive in the silver, lead, zinc, and methylen blue stains and shows a high ATPase activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Battaglia, E., andF. Maggini, 1968: The use of osmium for staining the nucleolus in squash technique. Caryologia21, 287–292.

    Google Scholar 

  • Bernhard, W., C. Frayssinet, C. Lafaye etE. Le Breton, 1965: Lésions nucléolaires précoces provoquées par l'aflatoxine dans les cellules hépatiques du rat. C. R. Acad. Sci. (Paris)261, 1785–1788.

    Google Scholar 

  • —, andN. Granbouland, 1968: Electron microscopy of the nucleoli in vertebrate cells. In: The Nucleus (A. Dalton andF. Haguenau, eds.), p. 1. New York and London: Acad. Press 1968.

    Google Scholar 

  • Conger, A. D., andL. M. Fairchild, 1953: A quick-freeze method for making smear slides permanent. Stain Techn.28, 281–283.

    PubMed  Google Scholar 

  • Chouinard, L. A., 1966: Nucleolar architecture in root meristematic cells ofAllium cepa. Nat. Cancer Inst. Monogr.23, 125–143.

    PubMed  Google Scholar 

  • Estable, C., etJ. R. Sotelo, 1951: Una nueva estructura celular: el nucleolonema. Inst. Inv. Cienc. Pub.1, 105–126.

    Google Scholar 

  • Fabbri, F., 1963: Ricerche al microscopio ottico ed elettronico sulla struttura del nucleolo di cellule meristematiche di alcune piante e considerazioni critiche sulla teoria dell „nucleolonema“. Caryologia16, 715–749.

    Google Scholar 

  • Fernández-Gómez, E., J. C. Stockert, J. F. López-Saez, andG. Giménez-Martín, 1969: Staining plant cell nucleoli with AgNO3 after formalin-hydroquinone fixation. Stain Techn.44, 48–49.

    PubMed  Google Scholar 

  • Fernández-Gómez, M. E., andJ. C. Stockert, 1970: Metallic impregnation of nucleoli in root-tip cells. The Nucleus13, 149–156.

    Google Scholar 

  • Giménez-Martín, G., andJ. C. Stockert, 1970: Nucleolar structure during the meiotic prophase inAllium cepa anthers. Z. Zellforsch.107, 551–563.

    PubMed  Google Scholar 

  • Haenel, U., 1950: Veränderung der Nukleolarsubstanz an Knochenmarkzellen bei perniziöser Anämie und ihre pathogenetische Bedeutung. Helv. Med. Acta17, 627–650.

    PubMed  Google Scholar 

  • Hay, E. D., 1968: Structure and function of the nucleolus in developing cells. In: The Nucleus (A. Dalton andF. Haguenau, eds.), p. 1. New York and London: Acad. Press.

    Google Scholar 

  • Izard, J., andW. Bernhard, 1962: Analyse ultrastructurale de l'argentophilie de nucléole. J. Microscopie1, 421–434.

    Google Scholar 

  • La Cour, L. F., 1966 a: The internal structure of nucleoli. Chromosomes Today1, 150–160.

    Google Scholar 

  • — andB. Wells, 1966 b: The loops and ultrastructure of the nucleolus ofIpheion uniflorum. Z. Zellforsch.82, 25–45.

    Google Scholar 

  • Lafontaine, J. G., andF. A. Lord, 1966: Ultrastructure and mode of formation of the nucleolus in plant cells. Natl. Cancer Inst. Monograph.23, 67–76.

    Google Scholar 

  • — 1968: Structural components of the nucleus in mitotic plant cells. In: The Nucleus (A. J. Dalton andF. Haguenau, eds.), p. 151. New York and London: Academic Press.

    Google Scholar 

  • Lettré, R., W. Siebs, andN. Paweletz, 1966: Morphological observations on the nucleolus of cells in tissue culture, with special regard to its composition. Natl. Cancer Inst. Monograph.23, 107–124.

    Google Scholar 

  • Love, R., andR. H. Liles, 1959: Differentiation of nucleoproteins by inactivation of protein bound amino groups and staining with toluidine blue and ammonium molybdate. J. Histochem. Cytochem.7, 164–181.

    PubMed  Google Scholar 

  • —,G. P. Studzinski, andR. J. Walsh, 1969: Nuclear, nucleolinar, and cytoplasmic acid phosphatases in uncultured mammalian cells. Exp. Cell Res.58, 62–72.

    PubMed  Google Scholar 

  • Miller, O. L., andB. R. Beatty, 1969: Nucleolar structure and function. In: Handbook of Molecular Cytology (A. Limade-Faria, ed.), p. 24. Amsterdam-London: North-Holland Publishing Co.

    Google Scholar 

  • Paweletz, N., S. Siebs undR. Lettré, 1967: Untersuchungen zur Argentaffin-Reaktion des Nukleolus. Z. Zellforsch. Mikroskop. Anat.76, 577–605.

    Google Scholar 

  • Shiffrin, N., andL. Levine, 1968: Cytochemical adenosinetriphosphatase in plant root meristem. J. Cell Sci.3, 423–436.

    Google Scholar 

  • Stockert, J. C., O. D. Colman, M. E. Fernández-Gómez, andG. Giménez-Martín, 1969: Nucleolar structure in root-tip cells ofAllium cepa. Experientia25, 1306–1307.

    PubMed  Google Scholar 

  • - -M. C.Risueño, and M. E.Fernández-Gómez: Nucleolar segregation by cold, 2–4 dinitrophenol, or anoxia in plant cells. Cytologia (in press).

  • —,M. E. Fernández-Gómez, J. M. Sogo, andJ. F. López-Sáez, 1970 a: Nucleolar segregation by adenosine 3′deoxyriboside (cordycepin) in root tip cells ofAllium cepa. Exp. Cell Res.59, 85–89.

    PubMed  Google Scholar 

  • —,G. Giménez-Martín, andJ. M. Sogo, 1970 b: Nucleolus and synaptonemal complexes in pachytene meiocytes ofAllium cepa. Cytobiologie2, 235–250.

    Google Scholar 

  • - and E.Esponda (in preparation): Nucleolar structure inDrosophila spermatocytes.

  • Studzinski, G. P., andR. Love, 1964: Nucleolar organelles shown by lead precipitation in unfixed cultured cells. Stain Techn.39, 397–401.

    PubMed  Google Scholar 

  • —, 1965: Selective binding of zinc by basic proteins of the HeLa cell nucleolus. J. Histochem. Cytochem.13, 365–375.

    PubMed  Google Scholar 

  • —,H. E. Reidbord, andR. Love, 1967: Enhancement of the affinity of nucleoli and chromosomes for zinc by treatment with chromic acid. Stain Techn.42, 301–306.

    PubMed  Google Scholar 

  • Tandler, C. J., 1959: The silver-reducing property of the nucleolus and the formation of prenucleolar material during mitosis. Exp. Cell Res.17, 560–564.

    PubMed  Google Scholar 

  • — andA. J. Solari, 1969: Nucleolar orthophosphate ions. J. Cell Biol.41, 91–108.

    PubMed  Google Scholar 

  • Vincent, W. S., 1955: Structure and chemistry of nucleoli. Intern. Rev. Cytol.4, 269–298.

    Google Scholar 

  • Wachstein, M., andE. Meisel, 1957: Histochemistry of hepatic phosphatases at a physiologic pH with special reference to the demonstration of bilecanaliculi. Amer. J. Clin. Path.27, 13–23.

    Google Scholar 

Download references

Author information

Author notes

  1. M. E. Fernändez-Gómez

    Present address: Departamento de Citología, Instituto de Biología Celular (C.S.I.C.), Velázquez, Madrid, Spain

Authors and Affiliations

  1. Departamento de Citología, Instituto de Biología Celular (C.S.I.C.), Velázquez, Madrid, Spain

    M. C. Risueño, G. Giménez-Martín & J. C. Stockert

Authors
  1. M. E. Fernändez-Gómez
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. C. Risueño
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. Giménez-Martín
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. C. Stockert
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fernändez-Gómez, M.E., Risueño, M.C., Giménez-Martín, G. et al. Cytochemical and ultrastructural studies on normal and segregated nucleoli in meristematic cells. Protoplasma 74, 103–112 (1972). https://doi.org/10.1007/BF01279204

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01279204

Keywords

Search

Navigation