Summary
Bellevalia romana (L.) Rchb., a monocotyledonous plant characterized by few (2 n=2 x=8) and very large chromosomes, is a useful subject for studying developmental problemsin vitro. Cytological analysis of callus revealed that the majority of cells were diploid, but the remaining cells had aneuploid nuclei with a wide range of chromosome numbers, tetraploid and haploid nuclei. The frequency of aneuploid and polyploid cells was higher in callus grown in the presence of 2,4-D than in callus grown in NAA plus BAP. These nuclei seemed to increase with the duration of culture. The chromosome number distribution as determined by chromosome counts in calli at different culture times was confirmed by DNA cytophotometry. Chromosome number mosaicism (mixoploidy and aneusomaty) also occurred in all root apices of 9 out of 46 plantlets regenerated from callusvia adventitious shoots.
Similar content being viewed by others
References
Bayliss MW, Gould AR (1974) Studies on the growth in culture of plant cell. XVIII. Nuclear cytology ofAcer pseudoplatanus suspension cultures. J exp Bot 25: 772–783
—, (1980) Chromosomal variability in plant tissues in culture. Intern Rev Cytol Suppl 11 A, pp 113–144
Bennici A, Buiatti M, D'amato F (1968) Nuclear conditions in haploidPelargonium in vivo andin vitro. Chromosoma 24: 194–201
—,D'amato F (1978)In vitro regeneration ofdurum wheat plants. 1. Chromosome numbers of regenerated plantlets. Z Pflanzenzücht 81: 305–311
—, (1979) Cytological chimeras in plants regenerated fromLilium longiflorum tissues grownin vitro. Z. Pflanzenzücht 82: 349–359
—, (1979) Cell differentiation in a diploid state inLilium longiflorum tissues. Protoplasma 101: 127–131
—,Baroncelli S, D'amato F (1979) Cytogenetics ofdurum wheat plants regeneratedin vitro. In: Israeli-Italian joint meeting on genetics and breeding of crop plants. Istituto Sperimentale per la Cerealicoltura, Roma, pp 177–188
—,Gennai D (1985) Cytophotometric DNA measurements on differentiated tissues of someLilium species. Caryologia 38: 221–227
Browers MA, Orton TJ (1982) Transmission of gross chromosomal variability from suspension cultures into regenerated celery plants. J Hered 73: 159–162
Caffaro L, Dameri RM, Profumo P, Bennici A (1982) Callus induction and plantlet regeneration inCichorium intybus L.: 1. A cytological study. Protoplasma 111: 107–112
Cionini PG, Bennici A, D'amato F (1978) Nuclear cytology of callus induction and developmentin vitro. 1. Callus fromVicia faba cotyledons. Protoplasma 96: 101–112
Collins GB, Legg PD, Kasperbauer MJ (1972) Chromosome numbers in anther-derived haploids in twoNicotiana species. J Hered 63: 113–118
Conger AD, Fairchild M (1953) A quick-freeze method for making smear slides permanent. Stain Technol 28: 281–283
D'amato F (1978) Chromosome number variation in cultured cells and regenerated plants. In:Thorpe TA (ed) Frontiers of plant tissue culture. University of Calgary, Calgary, pp 287–295
D'Amato F (1985) Cytogenetics of plant cell and tissue cultures and their regenerates. CRC Crit Rev Plant Sci (in press)
Doležel J, Novak FJ (1985) Karyological and cytophotometric study of callus induction inAllium sativum L. J Plant Physiol 118: 421–429
Heinz DJ, Mee GW, Nickell LG (1969) Chromosome number of someSaccharum species hybrids and their cell suspension cultures. Am J Bot 56: 450–459
Lupi MC, Bennici A, Gennai D (1985)In vitro culture ofBellevalia romana (L.) Rchb. 1. Plant regeneration through adventitious shoots and somatic embryos. Protoplasma 125: 185–189
Mar'yakhima IYA, Butenko RG (1974) Somatic reduction in a cabbage tissue culture. Cytol Genet 8: 74–77
Mix G, Wilson HM, Faroughi-Wehr B (1978) The cytological status of plants ofHordeum vulgare L. regenerated from microspore callus. Z Pflanzenzücht 80: 89–99
Ogura H (1976) The cytological chimeras in original regerates from tobacco tissue cultures and their offsprings. Jpn J Genet 51: 161–174
Phillips RL, Wang AS (1984) Chromosome analysis. In:Vasil IK (ed) Cell culture and somatic cell genetics of plants, vol I. Academic Press, Inc, New York, pp 712–727
Ramulu SK, Dijkhuis P, Roest S (1984) Genetic instability in protoclones of potato (Solanum tuberosum L. cv. “Bintje”): new type of variation after vegetative propagation. Theor Appl Genet 88: 515–519
Sacristan MD (1971) Karyotypic changes in callus cultures from haploid and diploid plants ofCrepis capillaris (L.). Chromosoma 33: 273–283
Shimada T, Tabata M (1967) Chromosome number in cultured pith tissue of tobacco. Jpn J Genet 47: 195–201
Skirvin RM (1978) Natural and induced variation in tissue culture. Euphytica 27: 241–266
Singh BD, Harvey BL (1975) Cytogenetic studies onHaplopappus gracilis cells cultured on agar and in liquid media. Cytologia 40: 347–354
Yamane Y (1975) Chromosomal variation in calluses induced inVicia faba andAllium cepa. Jpn J Genet 50: 353–355
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cavallini, A., Cremonini, R., Lupi, M.C. et al. In vitro culture ofBellevalia romana (L.) Rchb.. Protoplasma 132, 58–63 (1986). https://doi.org/10.1007/BF01275790
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01275790