Summary
With 6-bromo-2-naphthyl-β-D-glucoside as substrate, β-glucosidase activity has been histochemically localized in the lateral root meristems ofZea mays. Enzyme activity is highest in the epidermis and root cap of the young primordium. In contrast, enzyme activity is low in the parent root cortex, including the damaged areas around the lateral root. The results indicate that β-glucosidase is involved in the development of the lateral root rather than its penetration through the cortex.
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Ashford, A. E., 1970: Histochemical localization of β-glucosidase inZea mays. I. A simultaneous coupling azo dye technique for localization of β-glucosidase and β-galactosidase. Protoplasma71, 281–293.
—,W. G. Allaway, andM. E. McCully, 1972: Low temperature embedding in glycol methacrylate for enzyme histochemistry in plant and animal tissues. J. Histochem. Cytochem.20, 986–990.
—, andM. E. McCully, 1970 a: Localization of Naphthol AS-BI phosphatase activity in lateral and main root meristems of corn. Protoplasma70, 441–456.
—, 1970 b: Histochemical localization of β-glycosidases in roots ofZea mays. II. Changes in localization and activity of β-glucosidase in the main root apex. Protoplasma71, 389–402.
Bell, J. K., andM. E. McCully, 1970: A histological study of lateral root initiation and development inZea mays. Protoplasma70, 179–205.
Bonnett, H. T., 1969: Cortical cell death during lateral root formation. J. Cell Biol.40, 144–159.
Cohen, R. B., S. H. Rutenburg, K. C. Tsou, S. H. Woodbury, andA. M. Seligman, 1952: The colorimetric estimation of β-D-glucosidase. J. biol. Chem.195, 607–614.
Eberhardt, F., 1955: Über fluoreszierende Verbindungen in der Wurzel des Hafers. Ein Beitrag zum Problem der Wurzelausscheidungen. Z. Bot.43, 405–422.
Goodwin, R. H., andB. M. Pollock, 1954: Studies on Roots. I. Properties and distribution of fluorescent constituents inAvena roots. Amer. J. Bot.41, 516–520.
Harborne, J. B., 1967: Comparative biochemistry of the flavonoids. London: Academic Press.
Karas, I., and M. E.McCully, 1973: Further studies of the histology of lateral root development inZea mays. Protoplasma, in press.
Nevins, D. J., 1970: Relation of glycosidases to bean hypocotyl growth. Plant Physiol.46, 458–462.
Pridham, J. B., 1960: The formation and possible function of phenolic glycosides. Phenolics in Plants in Health and Disease (J. B. Pridham, ed.). London: Pergamon Press.
Richter, M., W. Wilms, andF. Scheffer, 1968: Determination of root exudates in sterile continuous flow culture. II. Short-term and long term variations of exudation intensity. Plant Physiol.43, 1747–1754.
Street, H. E., 1966: The physiology of root growth. Ann. Rev. Plant Physiol.17, 315–344.
Sutcliffe, J. F., andR. Sexton, 1968: β-Glycerophosphatase and lateral root development. Nature217, 1285.
Vančura, V., 1964: Root exudates of plants. 1. Analysis of root exudates of barley and wheat in their initial phases of growth. Plant and Soil21, 231–248.
Wahlroos, O., andA. I. Virtanen, 1959: The precursors of 6-methoxybenzosazolinone in maize and wheat plants, their isolation and some of their properties. Acta Chem. Scand.13, 1906–1908.
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Ashford, A.E., McCully, M.E. Histochemical localization of β-glycosidases in roots ofZea mays . Protoplasma 77, 411–425 (1973). https://doi.org/10.1007/BF01275718
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DOI: https://doi.org/10.1007/BF01275718