Abstract
The expression of the human cyclin B1 gene was investigated with Western blot analysis in human colorectal carcinomas and in adjacent non-neoplastic colorectal mucosas. Out of 41 cancers, 36 (88% of patients) showed much higher expression of cyclin B1 than did the non-neoplastic mucosa. Proliferating-cell nuclear antigen (PCNA) immunohistochemistry revealed that the labeling indexes of these cancer tissues were 47.3±11.3% while those of the mucosa were 15.6±5.5%. Only 5 cancers (12% patients) demonstrated the same expression level of cyclin B1 as the mucosa; however, the PCNA labeling indexes were 42.3±11% for the cancer tissue, compared to 12.6±2.4% for the mucosas. Southern blot analysis showed that there was no change of the cyclin B1 gene at the somatic DNA level in spite of its high expression at the protein level. These results proved that majority of colorectal cancers express high levels of cyclin B1, consistent with a high rate of cell proliferation, whereas a small fraction of these cancers lose control of cyclin B1 expression, diverging from their fast cell proliferation.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- PCNA :
-
proliferating-cell nuclear antiger
References
Akama Y, Yasui W, Yokozaki H, Kuniyasu H, Kitahara K, Ishikawa T, Tahara E (1995) Frequent amplification of the cyclin E in human gastric carcinomas. Jpn J Cancer Res 86:617–621
Blm N, Safford DM (1976) A general method for isolation of high molecular weight DNA from eukaryotes. Nucleic Acids Res 3:2303–2308
Clurman B, Roberts J (1995) Cell cycle and cancer. J Natl Cancer Inst 87:1499–1501
Gallant P, Nigg E (1994) Identification of a novel vertebrate cyclin: cyclin B2 shares properties with both A- and B-type cyclins. EMBO J 13:595–605
Hall PA, Levision DA, Woods AL, C.C.-W.Y., Kellock DB, Watkins JA, Barnes DM, Gillett CE, Camplejohn R, Dover R, Waseem NH, Lane DP (1990) Proliferation cell nuclear antigen (PCNA) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms. J Pathol 162:285–294
Jackman M, Firth M, Pines J (1995) Human cyclin B1 and B2 are localized to strikingly different structures: B1 to microtubules, B2 primarily to the Golgi apparatus. EMBO J 14:1646–1654
Jiang W, kahn SM, Tomita N, Zhang YJ, Lu SH, Weinstein IB (1992) Amplification and expression of the human cyclin D gene in esophageal cancer. Cancer Res 52:2980–2983
Keyomarsi K, Pardee AB (1993) Redundant cyclin overexpression and gene amplification in breast cancer cells. Proc Natl Acad Sci USA 90:1112–1116
Kosaka C, Sasaguki T, Masuda J, Zen K, Shimokado K, Yokota T, Ogata J (1993) Protein kinase C-mediated inhibition of cyclin A expression in human vascular endothelial cells. Biochem Biophys Res Commun 193:991–998
Lu K, Hunter T (1995) Evidence for a NIMA mitotic pathway in vertebrate cells. Cell 81:413–424
Murray A and Kirschner M (1989) Dominoes and clocks: the union of two view of the cell cycle. Science 246:615–621
Nishida N, Fukuda Y, Komeda T, Kita R, Sando T, Furukawa M, Amenomori M, Shibagaki I, Nakao K, Ikenaga M, Ishizaki K (1994) Amplification and overexpression of the cyclin D1 gene in aggressive human hepatocellular carcinoma. Cancer Res 54:3107–3110
Paterlini P, Suberville AM, Zindy F, Melle J, Sonnier M, Marie JP, Dreyfus F, Brechot C (1993) Cyclin A expression in human hematological malignancies: a new marker of cell proliferation. Cancer Res 53:235–238
Pines J (1993) Cyclins and cyclin-dependent kinases: take your partners. Trends Biochem Sci 18:195–197
Pines J, Hunter T (1989) Isolation of a human cyclin cDNA: evidence for cyclin mRNA and protein regulation in the cell cycle and for interaction with p34cdc2. Cell 58:833–846
Sambrook J, Fritsch EF, Maniatis T (1989a) Molecular cloning. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 18.47–18.75
Sambrook J, Fritsch EF, Maniatis T (1989b) Molecular cloning. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 9.24–9.58
Wang A, Yoshimi N, Yamauch A, Tarao M, Mori H (1996) Different expression patterns of cyclins A, D1 and E in human colorectal cancer. J Cancer Res Clin Oncol 122:122–126
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wang, A., Yoshimi, N., Ino, N. et al. Overexpression of cyclin B1 in human colorectal cancers. J Cancer Res Clin Oncol 123, 124–127 (1997). https://doi.org/10.1007/BF01269891
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01269891