Summary
Using ultra-filtration steps and HPLC-separation, a low molecular weight ligand of the benzodiazepine receptor was isolated from plasma of various mammalian species including man. The endogenous ligand acts on benzodiazepine receptors agonistically and apparently has a receptor affinity similar to Diazepam. The ligand is not identical with Diazepam as indicated by HPLC and UV-spectroscopy.
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Abbreviations
- GABA:
-
γ-aminobutyric acid
- EDTA-K2 :
-
ethylenediaminetetraacetic acid dipotassium salt
- Tris-HCl:
-
tris(hydroxymethyl)aminomethane hydrochloride
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Wildmann, J., Niemann, J. & Matthaei, H. Endogenous benzodiazepine receptor agonist in human and mammalian plasma. J. Neural Transmission 66, 151–160 (1986). https://doi.org/10.1007/BF01260910
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DOI: https://doi.org/10.1007/BF01260910