Abstract
Solid-phase extraction and liquid chromatography-tandem mass spectrometry are invaluable techniques for the determination of benzodiazepines and metabolites in biological matrices. The reason for using tandem mass spectrometry is to increase limits of detection without the need for chemical derivatization. Here we describe a technique for the detection of 26 benzodiazepines and metabolites at a detection limit of approximately 1–2 ng/mL in blood and 1–5 ng/mL in urine when screened using a data-dependent scan method.
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References
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Acknowledgements
This extraction procedure is derived from “Flunitrazepam, 7-Aminoflunitrazepam, and Desmethyl-flunitrazepam in Urine for GC/MS Confirmations Using 200 mg CLEAN SCREEN DAU Extraction Column”; first published by United Chemical Technologies (UCT) Inc., UCT has granted approval for the reprinting of this extraction procedure.
Disclaimer: This document is publication number 11-03 of the Laboratory of the Federal Bureau of Investigation (FBI). Names of commercial manufacturers are provided for identification purposes only and inclusion does not imply endorsement of the manufacturer, its products, or its services. The views expressed are those of the authors and do not necessarily reflect the official policy or position of the FBI or the US Government.
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© 2012 Springer Science+Business Media, LLC
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Morris-Kukoski, C.L., Schaff, J.E., Reda, L.J. (2012). Benzodiazepines and Metabolites from Biological Fluids by Liquid Chromatography Electrospray Tandem Mass Spectrometry. In: Langman, L., Snozek, C. (eds) LC-MS in Drug Analysis. Methods in Molecular Biology, vol 902. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-934-1_4
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DOI: https://doi.org/10.1007/978-1-61779-934-1_4
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-933-4
Online ISBN: 978-1-61779-934-1
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