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Immunohistochemical studies on the effect of capsaicin on spinal and medullary peptide and monoamine neurons using antisera to substance P, gastrin/CCK, somatostatin, VIP, enkephalin, neurotensin and 5-hydroxytryptamine

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Journal of Neurocytology

Summary

After neonatal treatment of rats with capsaicin, the spinal cord, the spinal trigeminal nucleus and spinal and trigeminal ganglia were analysed with immunohistochemistry using antisera to several peptides and 5-hydroxytryptamine. A marked decrease was observed in substance P-, cholecystokinin-, somatostatin- and VIP-like immunoreactivity present in the central branches of primary sensory neurons in the spinal cord and in substance P- and somatostatin-like immunoreactivity in sensory ganglion cells. No definite depleting effect of capsaicin could be established on 5-hydroxytryptamine and peptides, such as enkephalin and neurotensin, present in centrally originating fibres in the dorsal horn of the spinal cord. The results demonstrate that the effects of capsaicin are not confined to substance P immunoreactive primary sensory neurons. The possibility is discussed that capsaicin effects specifically functioning rather than chemically specific primary sensory neurons.

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Authors and Affiliations

  1. Department of Anatomy, University Medical School and Institute of Biophysics, Biological Research Center, Szeged, Hungary

    G. Jancsó, E. Kiraly & N. Halász

  2. Department of Histology, Karolinska Institutet, Stockholm

    T. Hökfelt & J. M. Lundberg

  3. Department of Physiology, Swedish University of Agricultural Sciences, Uppsala, Sweden

    G. Nilsson & L. Terenius

  4. Department of Pharmacology, Uppsala University, Uppsala, Sweden

    G. Nilsson & L. Terenius

  5. Department of Medical Biochemistry, Aarhus University, Aarhus, Denmark

    J. Rehfeld

  6. Department of Embryology and Anatomy, Katholieke Universiteit, Nijmegen, The Netherlands

    H. Steinbusch & A. Verhofstad

  7. Department of Anatomy, University of Minnesota, Minneapolis, Minnesota, USA

    R. Elde

  8. Department of Internal Medicine and Pharmacology, University of Texas, Dallas, Texas, USA

    S. Said

  9. The Salk Institute, La Jolla, San Diego, USA

    M. Brown

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  1. G. Jancsó
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  2. T. Hökfelt
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  8. J. Rehfeld
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  9. H. Steinbusch
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Jancsó, G., Hökfelt, T., Lundberg, J.M. et al. Immunohistochemical studies on the effect of capsaicin on spinal and medullary peptide and monoamine neurons using antisera to substance P, gastrin/CCK, somatostatin, VIP, enkephalin, neurotensin and 5-hydroxytryptamine. J Neurocytol 10, 963–980 (1981). https://doi.org/10.1007/BF01258524

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  • DOI: https://doi.org/10.1007/BF01258524

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