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Product formation during batch fermentation with recombinant Escherichia coli containing a runaway plasmid

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Abstract

The product formation during batch fermentation with recombinant E. coli containing a runaway replication plasmid has been examined. Theoretical modelling is combined with experimental work to study the effect of operating conditions. In particular the influence of induction profile has been investigated. High sensitivity to operating conditions is observed, and both model and experimental data illustrate the presence of very narrow limits for an optimal induction profile.

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Abbreviations

f i :

function for allocation of energy to the i'th reaction in the one substrate model

g i :

function for allocation of energy to the i'th reaction in the two substrate model

h :

function for inhibition by plasmid material

K i (h−1):

kinetic rate constant for the i'th reaction

k i (g/l):

saturation constants

K p (g P/g biomass):

saturation constant for recombinant protein synthesis

K s (g/l):

inhibition constant of glucose on acetate metabolism

K p,i (g P/g biomass):

inhibition constant of plasmid material on cellular activity

p (g/l):

extracellular acetic acid concentration

r i (h−1):

specific rate of i'th reaction

s (g/l):

extracellular glucose concentration

X i (g i/g biomass):

intracellular concentration of the i'th component

β ij :

stoichiometric coefficients for the i'th metabolic product in the j'th reaction

γ ij :

stochiometric coefficients for the i'th component in the biotic phase in the j'th reaction

ε i :

relative allocation of energy to the i'th reaction with growth on acetate compared with growth on glucose

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Authors and Affiliations

  1. Chr. Hansens Laboratory A/S, Bøge Allé 10-12, DK-2970, Hørsholm, Denmark

    K. Strudsholm

  2. Department of Biotechnology Block 223, Technical University of Denmark, DK-2800, Lyngby, Denmark

    J. Nielsen & C. Emborg

Authors
  1. K. Strudsholm
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  2. J. Nielsen
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  3. C. Emborg
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Strudsholm, K., Nielsen, J. & Emborg, C. Product formation during batch fermentation with recombinant Escherichia coli containing a runaway plasmid. Bioprocess Engineering 8, 173–181 (1992). https://doi.org/10.1007/BF01254234

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