Abstract
In the ribosomal DNA unit ofPleurotus cornucopiae, the rDNA coding regions are in the order 5′, 5S-18S-5.8S-25S, 3′, with the 5′ location of the 5S gene differing from its 3′ location found in other basidiomycetes. The most discriminating probe used to study the rDNA polymorphism consisted of a fragment that included the 5S, 18S and part of the 5.8S and 25S genes flanking three intergenic sequences. A high degree of rDNA polymorphism was observed in the sevenP. cornucopiae dikaryons studied. For the first time within a basidiomycete species, the restrictions maps distinguished two types of rDNA units (I and II). In each rDNA type, length variations in the external intergenic sequence IGS 1 located between the 25S and 5S genes allowed characterization of two different rDNA units in type I and four rDNA units in type II. This suggested that theP. cornucopiae rDNA units were derived from two kinds of ancestors (type I and II) by insertion or deletion events (100–700 bp) in the IGS 1. In four dikaryotic strains, two rDNA units of the same type (I or II) differing only by the IGS 1 length, were found in a similar number of copies, and presented a meiotic segregation in homokaryotic progeny. In one progeny, some homokaryotic strains possessed two different rDNA units: one with a high copy number and another with a lower one, showing that two different rDNA units could coexist in a single nucleus.
Similar content being viewed by others
References
Bell GI, De Gennaro LJ, Gelfand DH, Bishop RJ, Valenzuela P, Rutter WJ (1977) Ribosomal RNA genes ofSaccharomyces cerevisiae. J Biol Chem 22:8118–8125
Buckner B, Novotny CP, Ullrich RC (1988) Organization of the ribosomal RNA genes ofSchizophyllum commune. Curr Genet 13: 417–424
Cassidy JR, Moore D, Lu BC, Pukkila PJ (1984) Unusual organization and lack of recombination in the ribosomal RNA genes ofCoprinus cinereus. Curr Genet 8:607–613
De Vries OMH, Hoge JHC, Wessels JGH (1980) Regulation of the pattern of protein synthesis inSchizophyllum commune. Dev Biol 74:22–36
Field GF, Olsen GJ, Lane DJ, Giovanni SJ, Ghiselin MT, Raff EC, Pace NR, Raff RA (1988) Molecular phylogeny of the animal kingdom. Science 239:748–753
Garber RC, Turgeon BG, Selker EU, Yoder OC (1988) Organization of ribosomal RNA genes in the fungusCochliobolus heterostrophus. Curr Genet 14:573–582
Hanahan D (1985) Techniques for transformation ofEscherichia coli. In: Glover DM (ed) DNA cloning: a practical approach. IRL Press, Oxford-Washington, pp 1–47
Hintz WE, Anderson JB, Horgen PA (1989) Relatedness of three species ofAgaricus inferred from restriction fragment length polymorphism analysis of the ribosomal DNA repeat and mitochondial DNA. Genome 32:173–178
Iraçabal B, Labarère J (1993) Comparison of polymorphism and phenetic variability as determined by the study of hydrolases and oxidoreductases in two cultivated mushrooms,Agaricus bisporus andPleurotus cornucopiae. Exp Mycol 17:90–102
Iraçabal B, Roux P, Labarère J (1991) Study of enzyme polymorphism inAgaricus andPleurotus species for characterization and genetic improvement. In: Maher MJ (ed) Science and cultivation of edible fungi. Academic Press, Rotterdam, pp 37–41
Arère J, Noël T, Imbernon M (1989) Selection and genetic analysis of antibiotic-resistant mutant strain inAgrocybe aegerita. Mushroom Sci 12:161–174
Bang EO, Dawid IB (1980) Repeated genes in eukaryotes. Annu Rev Biochem 49:727–764
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning — a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
Noël T, Labarère J (1989) Isolation of DNA fromAgrocybe aegerita for the construction of a genomic library inEscherichia coli. Mushroom Sci 12:187–201
Noël T, Salvado JC, Labarère J (1991) Specific aspects of molecular cloning inAgrocybe aegerita and their practical applications. In: Maher MJ (ed) Science and cultivation of edible fungi. Academic Press, Rotterdam, pp 71–77
Pukkila PJ, Cassidy JR (1987) Varying patterns of ribosomal RNA gene organisation in basidiomycetes. In: Rayner ADM, Brasier CM, Moore D (eds) Symp Br Myco Soc. (Evolutionary biology of the fungi). Cambridge University Press, London, pp 75–82
Ranzani GN, Bernini LF, Crippa M (1984) Inheritance of rDNA spacer lengths variants in man. Mol Gen Genet 196:141–145
Raper JR, Hoffman RM (1974)Schizophyllum commune. In: King RC (ed) Handbook of Genetics. Plenum Press, New York, pp 597–626
Rogers SO, Honda S, Bendich AJ (1986) Variation in the ribosomal RNA genes among individuals ofVicia fibia. Plant Mol Biol 6: 339–345
Rogers SO, Rehner S, Bledsoe C, Mueller GJ, Ammirati JF (1989) Extraction of DNA from basidiomycetes for ribosomal DNA hybridizations. Can J Bot 67:1235–1243
Santoni S, Bervillé A (1992) Characterization of the nuclear ribosomal DNA units and phylogeny of Beta L. wild forms and cultivated beets. Theor Appl Genet 83:533–542
Specht CA, Novotny CP, Ullrich RC (1984) Strain specific differences in ribosomal DNA from the fungusSchizophyllum commune. Curr Genet 8:219–222
Van Heerikhuizen H, Ykema A, Klootwijk J, Gaillardin C, Ballas C, Fournier P (1985) Heterogeneity in the ribosomal RNA genes of yeastYarrowia lipolytica; cloning and analysis of two size classes of repeats. Gene 39:213–222
Vilgalys R, Gonzalez D (1990) Organisation of ribosomal DNA in the basidiomyceteThanatephorus praticola. Curr Genet 18:277–280
Wu MMJ, Cassidy JR, Pukkila PJ (1983) Polymorphisms in DNA ofCoprinus cinereus. Curr Genet 7:385–392
Author information
Authors and Affiliations
Additional information
Communicated by G. Wenzel
Rights and permissions
About this article
Cite this article
Iraçabal, B., Labarère, J. Restriction site and length polymorphism of the rDNA unit in the cultivated basidiomycetePleurotus cornucopiae . Theoret. Appl. Genetics 88, 824–830 (1994). https://doi.org/10.1007/BF01253992
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01253992