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RFLP linkage analysis of the Cf-4 and Cf-9 genes for resistance toCladosporium fulvum in tomato

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Abstract

Four different populations segregating for one of the two closely linked (possibly allelic) tomato disease resistance genes to the fungusCladosporium fulvum,Cf-4 andCf-9, were generated and analysed for recombination frequencies between theCf-genes and restriction fragment length polymorphism (RFLP) loci. The population consisting of F2 progeny from the interspecific crossLycopersicon esculentum carryingCf-9 ×L. pennellii was identified as the most useful for RFLP mapping of theCf-4/9 locus and an RFLP map around this locus was constructed mainly using this population. The two closest markers identified were CP46, 2.6 cM distal, and a group of 11 markers including TG236, 3.7 cM proximal toCf-4/9. A polymerase chain reaction (PCR)-based procedure for the rapid identification of recombination events between these two markers was developed. The regions of foreign DNA introgression surroundingCf-4 andCf-9 in near-isogenic lines were delimited.

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Author notes

  1. P. J. Balint-Kurti

    Present address: Laboratory of cellular and developmental biology, NIDDK, National Institutes of Health, 9000 Rockville Pike, 20892, Bethesda, Maryland, USA

Authors and Affiliations

  1. Sainsbury Laboratory, John Innes Centre for Plant Science Research, Colney Lane, NR4 7UH, Norwich, England, UK

    P. J. Balint-Kurti, M. S. Dixon, D. A. Jones, K. A. Norcott & J. D. G. Jones

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  1. P. J. Balint-Kurti
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  2. M. S. Dixon
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  3. D. A. Jones
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  4. K. A. Norcott
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  5. J. D. G. Jones
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Communicated by M. Koornneef

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Balint-Kurti, P.J., Dixon, M.S., Jones, D.A. et al. RFLP linkage analysis of the Cf-4 and Cf-9 genes for resistance toCladosporium fulvum in tomato. Theoret. Appl. Genetics 88, 691–700 (1994). https://doi.org/10.1007/BF01253972

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  • DOI: https://doi.org/10.1007/BF01253972

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