Summary
The transformation of hamster embryo cells with adenovirus type 12 is dependent on a variety of secondary factors. Conditions can be selected that favor development of primary transformed colonies, thus permitting the reproducible assay of viral transformation efficiency; other conditions favor the metastatic spreading of transformed cells, thus facilitating the establishment of transformed cell lines.
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McBride, W. D., andA. Wiener:In vitro transformation of hamster kidney cells by human adenovirus type 12. Proc. Soc. exp. Biol. (N.Y.)115, 870–874 (1964).
Pope, J. H., andW. P. Rowe: Immunofluorescent studies of adenovirus 12 tumor and of cells transformed or infected by adenovirus. J. exp. Med.120, 577–588 (1964).
Rafajko, R. R.: Routine establishment of serial lines of hamster embryo cells transformed by adenovirus type 12. J. nat. Cancer Inst.38, 581–591 (1967).
Stich, H. T., G. L. Van Hoosier, andJ. J. Trentin: Viruses and mammalian chromosomes. J. exp. Cell Res.34, 400–403 (1964).
Freeman, A. E., P. H. Black, andR. Wolford: Adenovirus type 12 — rat embryo transformation system. J. Virol.1, 362–367 (1967).
Schell, K., Jean Maryak, andMargarete Schmidt: Adenovirus transformation of hamster embryo cells: I. Assay conditions. Arch. ges. Virusforsch.24, 332–341 (1968).
Malinin, G. I., Margarete Schmidt, andK. Schell: Cytochemical observations on adenovirus type 12 transformed hamster embryo cells (in press).
Schell, K., Jean Maryak, andMargarete Schmidt: Adenovirus transformation of hamster embryo cells: II. Inoculation conditions. Arch. ges. Virusforsch.24, 342–351 (1968).
Evans, V. J., J. C. Bryant, M. C. Fioramonti, W. T. McQuilkin, K. K. Sanford, andW. R. Earle: Studies of nutrient media for tissue cells in vitro. I. A protein-free chemically defined medium for cultivation of strain L cells. J. nat. Cancer Res.16, 77–86 (1956).
Morgan, J. F., H. J. Morton, andR. C. Parker: Nutrition of animal cells in tissue culture. I. Initial studies on a synthetic medium. Proc. Soc. exp. Biol. (N.Y.)73, 1–8 (1950).
Eagle, H.: Amino acid metabolism in mammalian cell cultures. Science130, 432–437 (1959).
Earle, W. R.: Production of malignancyin vitro. IV. The mouse fibroblast cultures and changes seen in the living cells. J. nat. Cancer Inst.4, 165–212 (1943).
Leibovitz, A.: The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. Amer. J. Hyg.78, 173 (1963).
Todaro, G. J., H. Green, andM. R. Switt: Susceptibility of human diploid fibroblast strain to transformation by SV40 virus. Science153, 1252–1254 (1966).
Merchant, D. J., R. H. Kahn, andW. Murphy, Jr.: “Handbook of Cell and Organ Culture”, Burgess Publishing Company, Minneapolis, Minnesota (1964).
Schell, K., Jean Maryak, K. J. Lynch, A. A. Kindurys, andMargarete Schmidt: Adenovirus transformation of hamster embryo cells: IV. Morphological changes induced by various adenoviruses (in press).
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This work was supported by the National Institutes of Health, National Institute of Allergy and Infectious Diseases (Contract PH 43-62-200) and the National Cancer Institute (Contract PH 43-64-941).
The assistance ofJanice Young is appreciated.
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Schell, K., Maryak, J. & Schmidt, M. Adenovirus transformation of hamster embryo cells. Archiv f Virusforschung 24, 352–360 (1968). https://doi.org/10.1007/BF01243076
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DOI: https://doi.org/10.1007/BF01243076