Summary
The syncytium-producing activity of herpes simplex virus was shown to be a result of a toxic activity of certain viral components present on the viral particles as well as free. The physicochemical properties of the syncytium-producing components of herpesvirus suggest that they are lipoprotein in nature. The rapid appearance of the syncytium-producing activityin tissue culture prior to the appearance of infectious virus (32,33) and the presence of antibodies in human and rabbit sera was demonstrated. The lability of syncytium-producing activity to pH values below 4 or above 10, heating at 56°C for 20 minutes, UV irradiation, repeated freezing and thawing and various chemical agents such as ether and acetone, to all of which certain other antigenic components of this virus are stable, was demonstrated. Syncytia appeared best in primary rabbit kidney, less well in baby hamster kidney and not in human embryo kidney, diploid or amnion cells or mouse MCN cells.
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This investigation was supported by research grant (AI-05612) from National Institute of Allergy and Infectious Diseases, U.S. Public Health Service, Bethesda, MD, U.S.A.
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Tokumaru, T. The nature of toxins of herpes simplex virus. Archiv f Virusforschung 24, 104–122 (1968). https://doi.org/10.1007/BF01242905
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DOI: https://doi.org/10.1007/BF01242905