Abstract
Glucoamylase production inAspergillus terreus was induced, in order, by glucose, cellobiose, sorbitol, sucrose, α-methyl mannoside and α-methyl glucoside. Optimal induction was at 38°C, pH 4.0 and with 8 mg glucose/ml. Cycloheximide at 10 μg/ml completely inhibited induction indicatingde novo protein synthesis was involved in induction of glucoamylase.
Résumé
La production de gluco-amylase est induite chezAspergillus terreus, en ordre décroissant, par le glucose, le celloboise, le sorbitol, le sucrose, l'α-methylmannoside et l'α-methylglucoside. L'induction est optimum à 38°C, pH 4.0 et en présence de 8 mg de glucose parml. La cycloheximide à 10 μg par ml inhibe complètement l'induction, ce qui implique use synthèse de protéinede novo dans l'induction de la gluco-amylase.
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References
Ali, S., Mahmood, S., Alam, R. &Hossain, Z. 1989 Culture condition for production of glucoamylase from rice bran byAspergillus terreus.MIRCEn Journal of Applied Microbiology and Biotechnology. 5, 525–532.
De Mot, R. &Verachtert, H. 1986 Enhanced production of amylase and glucoamylase by amylolytic yeasts using cyclodextrin as carbon source.Applied Microbiology and Biotechnology,24, 459–462.
Ju-Nichi, A., Fredrico, B.W., Kazukai, O. &Susumu, H. 1985 Raw starch digesting enzyme ofAspergillus spp. K-27.Denpu Kagaku 32, 128–135.
Kanlayakrit, W., Ishimatsu, K., Nakao, M. &Hayashida, S. 1987 Characteristics of raw starch-digesting glycoamylase from thermophilicRhizomucor pusillus.Journal of Fermentation Technology 65, 379–385.
Manjunath, P., Shenoy, B.C. &Raghuvendra Rao, M.R. 1983 Fungal glucoamlyases.Journal of Applied Biochemistry 5, 235–260.
Nelson, N. 1944 A photometric adaption of the Somogyi for the determination of glucose.Journal of Biological Chemistry 153, 375–380.
Pestana, F. &Castello, J. 1985 Glucoamylase production byAspergillus awamori on rice flour medium and partial characterisation of the enzyme.MIRCEN Journal of Applied Microbiology and Biotechnology 1, 225–237.
Saito, N. &Yamamoto, K. 1975 Regulating factors affecting α-amylase production inBacillus licheniformis.Journal of Bacteriology 121, 848–856.
Somogyi, M. 1952 Notes on sugar determination.Journal of Biological Chemistry 195, 19–23.
Windish, M.W. &Mhatre, N.S. 1965 Microbial amylases.Advances in Applied Microbiology 7, 273–298.
Yabuki, M., Ono, N., Hoshino, K. &Fukui, S. 1977 Rapid induction of α-amylase by nongrowing mycelia ofAspergillus oryzae.Applied and Environmental Microbiology 34, 1–6.
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Ali, S., Hossain, Z., Mahmood, S. et al. Induction of glucoamylase production by non-starchy carbohydrates inAspergillus terreus . World J Microbiol Biotechnol 6, 19–22 (1990). https://doi.org/10.1007/BF01225349
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DOI: https://doi.org/10.1007/BF01225349