Summary
Diurnal plasma glucose levels have been studied two hourly in streptozotocin diabetic rats during insulin treatment. Protamine Zinc Insulin induced a very steep fall in plasma glucose level from 359±100 (SD) mg/100 ml to 91± 49 mg/100 ml within two hours. Plasma glucose was then low (13–60 mg/100 ml) until after 18 hours when an equally steep rise in glucose concentration ocurred. Six other insulin preparations and several insulin treatment regimens were tested with the aim of normalising the 24 hour plasma glucose profile of streptozotocin diabetic rats. One preparation, a non-commercial, very long acting Ultralente NOVO (Mc, ox pH 5.5) yielded a diurnal plasma glucose profile reasonably close to normal when it was administered once a day and when the dose was based on daily blood glucose measurements. Mean plasma glucose was 122±55 mg/100 ml with a nadir of 55±15 and a maximal of 187±99 mg/100 ml.
Article PDF
Similar content being viewed by others
References
Blackshear, P. J., Alberti, K. G. M. M.: Experimental diabetic ketoacidosis. Biochem. J.138, 107–117 (1974)
Christensen, N. J.: Notes on the glucose oxidase method. Scand. J. Clin. Lab. Invest.19, 379–384 (1967)
Engermann, R. L., Bloodworth, J. B. M., Nelson, S.: Relationship of microvascular disease in diabetes to metabolic control. Diabetes26, 760–769 (1977)
Gabbay, K. H.: Role of sorbitol pathway in neuropathy. In: Early Diabetes. Camerini-Dávalos, R. A.; Cole, H. S. (Eds.), pp. 417–424. New York, London: Academic Press 1973
Greene, D. A., De Jesus, P. V., Winegrad, A. I.: Effects of insulin and dietary myoinositol on impared periferal motor nerve conduction velocity in acute streptozotocin diabetes. J. Clin. Invest.55, 1326–1336 (1975)
Hägg, E.: Influence of insulin treatment on glomerular changes in rats with long-term alloxan diabetes. Acta Pathol. Microbiol. Scand. [A]82, 228–234 (1974)
Jacobsen, J.: Peripheral nerves in early experimental diabetes. Expansion of the endoneurial space as a cause of increased water content. Diabetologia14, 113–119 (1978)
Jarrett, R. J., Keen, H., Hardwick, C.: ‘Instant’ blood sugar measurement using dextrostix and a reflectance meter. Diabetes19, 724–726 (1970)
Rasch, R.: Prevention of diabetic glomerulopathy in streptozotocin diabetic rats by insulin treatment: Kidney size and glomerular volume. Diabetologia16, 125–128 (1979)
Rasch, R.: Prevention of diabetic glomerulopathy in streptozotocin diabetic rats by insulin treatment: Glomerular basement membrane thickness. Diabetologia (in press)
Schlichtkrull, J.: Insulin Clystals, p. 97. Thesis, University of Copenhagen. Copenhagen: Ejnar Munksgaards Publishers 1958
Schmidt, R. E., Matschinsky, F. M., Godfrey, D. A., Williams, A. D., McDougal, D. B.: Fast and slow axoplasmic flow in sciatic nerve of diabetic rats. Diabetes24, 1081–1085 (1975)
Steiner, D. F., Rauda, V., Williams, R. H.: Severe ketoacidosis in the alloxan diabetic rat. Endocrinology68, 809–817 (1961)
Sturtevant, F. M.: Effect of insulin on glucosuria, polyuria and food intake in alloxanized rats. Diabetes5, 388–390 (1956)
Ørskov, H., Olsen, S. T., Rafaelson, O. J., Lundbæk, K.: Kidney lesions in the rat with severe long term alloxan diabetes. Diabetologia1, 172–179 (1965)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rasch, R. Control of blood glucose levels in the streptozotocin diabetic rat using a long-acting heat-treated insulin. Diabetologia 16, 185–190 (1979). https://doi.org/10.1007/BF01219796
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01219796