Abstract
Insulin-like growth factors are potent mitogenic factors in human lung cancer in vitro, acting via specific receptors. Using monoclonal antibodies we demonstrate the expression of insulin-like growth factor receptor I in bronchial epithelial cells of normal lung and in primary lung cancer (22/24 cases), being most prominent in squamous cell carcinoma. Electron microscopy on lung cancer cell lines reveals a distinct reaction pattern on the plasma membrane. Immunoreaction with a specific antibody directed against the insulin-like growth factor receptor II suggests a weak expression in primary lung cancer. Our findings underline the significance of the autocrine pathway of insulin-like growth factors in lung cancer.
Similar content being viewed by others
Abbreviations
- IGF:
-
insulin-like growth factor
References
Bonneterre J, Peyrat JP, Beuscart R, Demaille A (1990) Prognositic significance of insulin-like growth factor I receptors in human breast cancer. Cancer Res 50:6931–6935
Braulke T, Gartung C, Hasilik A, Figura K von (1987) Is movement of mannose 6-phosphate-specific receptor triggered by binding of lysosomal enzymes? J Cell Biol 104:1735–1742
Braulke T, Causin C, Waheed A, Junghans U, Hasilik A, Maly P, Humbel R, Figura K von (1988) Mannose 6-phosphate/insulin-like growth factor II receptor: distinct binding sites for mannose 6-phosphate and insulin-like growth factor II. Biochem Biophys Res Commun 150:1287–1293
Carnedy DN, Gazdar AF, Bepler G, Guccion JG, Marangos PJ, Moody TW, Zweig MH, Minna JD (1985) Establishment and identification of small cell lung cancer lines having classic and variant features. Cancer Res 45:2913–2923
El-Badry O, Helmann LJ, Chatten J, Steinberg SM, Evabs AE, Israel MA (1991) Insulin-like growth factor II-mediated proliferation of human neuroblastoma. J Clin Invest 87:648–657
Foekens JA, Portengen H, Janssen M, Klijn J (1989) Insulin-like growth factor-I receptors and insulin-like growth factor I like activity in human primary breast cancer. Cancer 63:2139–2147
Furnaletto RW, Di Carlo JN (1984) Somatomedin-C receptors and growth effects in human breast cells maintained in long-term tissue culture. Cancer Res 44:2122–2128
Havemann K, Rotsch M, Schöneberger HJ, Erbil C, Hennig C, Jacques G (1990) Growth regulation by insulin-like growth factors in lung cancer. J Steroid Biochem Mol Biol 37:877–882
Jaques G, Rotsch M, Wegmann C, Worsch U, Maasberg M, Havemann K (1988) Production of immunoreactive insulin-like growth factor I and response to exogenous IGF I in small cell lung cancer cell lines. Exp Cell Res 176:336–343
Kiefer P, Jaques G, Schoeneberger J, Heinrich G, Havemann K (1991) Insulin-like growth factor binding protein expression in human small cell lung cancer cell lines. Exp Cell Res 192:414–417
Kull FC, Jacobs S, Su Y, Svoboda ME, Van Wyk JJ, Cuatrescas P (1983) Monoclonal antibodies to receptors for insulin and somatomedin C. J Biol Chem 228:6561–6565
Macaulay VM, Everard MJ, Teale JD, Trott PA, Van Wyk JJ, Smith IE, Millar JL (1990) Autocrine function for insulin-like growth factor I in human small cell lung cancer cell lines and fresh tumor cells. Cancer Res 50:2511–2517
Minuto F, Del Monte P, Barreca A, Fortini P, Cariola G, Catrambone G, Giordano G (1986) Evidence for an increased somatomedin-C/insulin like growth factor I content in primary human lung tumors. Cancer Res 46:985–988
Morgan DO, Edman JC, Standring DN, Fried VA, Smith MC, Roth RA, Rutter WJ (1987) Insulin-like growth factor II receptor as a multifunctional binding protein. Nature 329:301–307
Nakanishi Y, Mulshine JL, Kaspazyk PG, Natale RB, Maneckjee R, Avis I, Treston AM, Gazdar AF, Minna JD, Cuttita F (1988) Insulin-like growth factor I can mediate autocrine proliferation of human small cell lung cancer cell lines in vivo. J Clin Invest 82:354–359
Osborne CK, Coronado EB, Kitten LJ, Arteaga CI, Fuqua SAW, Ramasharma K, Marshall M, Li CH (1989) Insulin-like growth factor-II (IGF-II): a potential autocrine/paracrine growth factor for human breast cancer acting via the IGF-I receptor. Mol Endocrinol 3:1701–1709
Reynolds ES (1963) The use of lead citrate at high pH as an electronopaque stain in electron microscopy. J Cell Biol 12:207–213
Shigematsu K, Kataoka Y, Kamio T, Kurihara M, Niwa M, Tsuchiyama H (1990) Partial characterization of insulin-like growth factor I in primary human lung cancers using immunohistochemical and receptor autoradiographic technique. Cancer Res 50:2481–2484
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kaiser, U., Schardt, C., Brandscheidt, D. et al. Expression of insulin-like growth factor receptors I and II in normal human lung and in lung cancer. J Cancer Res Clin Oncol 119, 665–668 (1993). https://doi.org/10.1007/BF01215985
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01215985