Abstract
N-Acetylcysteine, known as a radical scavenger, was examined for its influence on the radiotolerance of progenitor cells of granulocytopoiesis. Added before and after irradiation in a dose of 2 mg/ml to suspension cultures of non-adherent low-density human bone marrow cellsN-acetylcysteine (AcCys) clearly improved the survival. TheD 0 value of the survival curve for granulocyte/macrophage colony-forming cells increased by a factor of 1.56 as compared to non-treated control suspensions. The improvement of radiation tolerance is probably not only based on the radical scavenger properties (radioprotective component) of AcCys, but also on the support of repair processes.
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Abbreviations
- AcCys:
-
N-acetylcysteine
- GM-CFC:
-
granulocyte/macrophage colony-forming cells
- FCS:
-
fetal calf serum
- HBSS:
-
Hanks balanced salt solution
- MNC:
-
mononuclear cells
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Work supported by the Bundesminister für Umwelt, Naturschutz und Reaktorsicherheit (St.Sch.1119)
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Selig, C., Nothdurft, W. & Fliedner, T.M. Radioprotective effect ofN-acetylcysteine on granulocyte/macrophage colony-forming cells of human bone marrow. J Cancer Res Clin Oncol 119, 346–349 (1993). https://doi.org/10.1007/BF01208843
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DOI: https://doi.org/10.1007/BF01208843