Skip to main content
Log in

Nachweis gentechnologisch veränderter Pflanzen mittels der Polymerase Kettenreaktion (PCR) am Beispiel derFlavr Savr TM-Tomate

Detection of genetically engineered plants by polymerase chain reaction (PCR) using theFlavr Savr TM tomato as an example

  • Originalarbeit
  • Published:
Zeitschrift für Lebensmittel-Untersuchung und Forschung Aims and scope Submit manuscript

Abstract

In this study, a practical approach for the detection of a genetically engineered tomato is demonstrated by polymerase chain reaction (PCR) assays. TheFlavr Savr TM tomato available on the market which was used as an example, contains a resistance gene for the antibiotic kanamycin (kansur) and a gene construct for the inhibition of fruit ripening and softening (antisense polygalacturonase). The presence of these DNA's could only be detected in the genetically engineered tomato.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Literatur

  1. Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA (1988) Science 239: 481–487

    Google Scholar 

  2. Candrian U (1994) Mitt Gebiete Lebensm Hyg 85: 704–718

    Google Scholar 

  3. Candrian U, Niederhauser Ch, Höfelein Ch, Bühler H-R, Müller U, Lüthy J (1991) Mitt Gebiete Lebensm Hyg 82: 539–558

    Google Scholar 

  4. Allmann M, Höfelein C, Köppel E, Lüthy J, Meyer R, Niederhauser C, Wegmüller B, Candrian U (1995) Res Microbiol 146: 85–97

    PubMed  Google Scholar 

  5. Allmann M, Candrian U, Höfelein Ch, Lüthy J (1993) Z Lebensm Unters Forsch 196: 248–251

    PubMed  Google Scholar 

  6. Meyer R, Candrian U, Lüthy J (1993) Mitt Gebiete Lebensm Hyg 84: 112–121

    Google Scholar 

  7. Meyer R, Candrian U, Lüthy J (1994) J Assoc Off Anal Chem 77: 617–622

    Google Scholar 

  8. Meyer R, Höfelein Ch, Lüthy J, Candrian U (1995) J AOAC Intern (im Druck)

  9. FDA (1994) Fed Reg 59: 26700–26711

    Google Scholar 

  10. Redenbaugh K, Lindemann J (1994) Proceedings, Basel Forum on Biosafety, Basel, CH BATS, 23–34

  11. Sheehy RE, Pearson J, Brady CJ, Hiatt WR (1987) Mol Gen Genet 208: 30–36

    Google Scholar 

  12. Redenbaugh K, Hiatt W, Martineau B, Kramer M, Sheehy R, Sanders R, Houck C, Emlay D (1992) Safety Assessement of Genetically-Engineered Fruits and Vegetables: a case study of the Flavr SavrTM tomato. Boca Raton, FL, CRC Press

    Google Scholar 

  13. Sheehy RE, Kramer M, Hiatt WR (1988) Proc Natl Acad Sci USA 85: 8805–8809

    Google Scholar 

  14. Smith CJS, Watson CF, Ray J, Bird CR, Morris PC, Schuch W, Grierson D (1988) Nature 334: 724–726

    Google Scholar 

  15. Gardner RC, Howarth AJ, Hahn P, Brown-Luedi M, Shepherd RJ, Messing J (1981) Nucleic Acids Res 12: 2871–2888

    Google Scholar 

  16. Fillatti JJ, Kiser J, Rose R, Comai L (1987) Bio/Technology 5: 726–730

    Google Scholar 

  17. Goldsbrough AP, Lastrella CN, Yoder JI (1993) Bio/Technology 11: 1286–1292

    Google Scholar 

  18. Bayley CC, Morgan M, Dale EC, Ow DW (1992) Plant Mol Biol 18: 353–361

    PubMed  Google Scholar 

  19. Jongedijk E, de Sutter AAJM, Stolte T, Van den Elzen PJM, Cornelissen BJC (1992) Bio/Technology 10: 422–429

    PubMed  Google Scholar 

  20. Padegimas L, Shul'ga OA, Skryabin KG (1993) Mol Biol 27: 583–585

    Google Scholar 

  21. Beck E, Ludwig G, Auerswald EA, Reiss B, Schaller H (1982) Gene 19: 327–336

    PubMed  Google Scholar 

  22. Barker RF, Harberd MG, Jarvis MG, Flavell RB (1988) J Mol Biol 201: 1–17

    PubMed  Google Scholar 

  23. Maniatis T, Fritsch EF, Sambrook J (1989) Molecular-cloning. Cold Spring Harbor, NY

    Google Scholar 

  24. Blake NK, Ditterline RL, Stout RG (1991) Crop Sci 31: 1686–1688

    Google Scholar 

  25. Oeller PW, Min-Wong L, Taylor LP, Pike DA, Theologis A (1991) Science 254: 437–439

    PubMed  Google Scholar 

  26. Golovkin MV, Abraham M, Morocz S, Bottka S, Feher A, Dudits D (1993) Plant Sci 90: 41–52

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Meyer, R. Nachweis gentechnologisch veränderter Pflanzen mittels der Polymerase Kettenreaktion (PCR) am Beispiel derFlavr Savr TM-Tomate. Z Lebensm Unters Forch 201, 583–586 (1995). https://doi.org/10.1007/BF01201590

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01201590

Navigation