Abstract
A thermostable, salt-tolerant amylase was produced byBacillus sp. 64, with maximum amylase production (8.0 U/ml culture filtrate) after 24-h growth. Partially purified amylase was stable at 60°C for 30 min and 80% of the original activity was retained when incubated in 5m NaCl over 24 h. Starch or dextrin was the best carbon source and peptone the best nitrogen source for the production of the enzyme. Amylase was secreted over a wide pH range (5 to 11) with the maximum activity between pH 7 and 8. Ca2+ and Mg2+ stimulated growth and enzyme production.
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Bernfeld, P. 1955 Amylases α/β.Methods in Enzymology1, 149–158.
Bezbaruah, R.L., Gogoi, B.K., Pilai, K.R. & Nigam, J.N. 1991 Amylase production by threeBacillus strains active at alkaline pH.Journal of Basic Microbiology31, 13–20.
Boyer, E.W. & Ingle, M.B. 1972 Extracellular alkaline amylase fromBacillus sp.Journal of Bacteriology110, 992–1000.
Buonocore, V., Caporale, C., De Rosa, M. & Gambacorta, A. 1976 Stable inducible thermoacidophilic α-amylase fromBacillus acidocaldarius.Journal of Bateriology128, 515–521.
Fogarty, W.M. 1983 Microbial amylases. InMicrobial Enzymes and Biotechnology, ed Fogarty, W.M. pp. 1–92. London and New York: Applied Science Publishers.
Linardi, V.R. & Machado, K.M. 1990 Production of amylases by yeasts.Canadian Journal of Microbiology36, 751–753.
Moseley, M.H. & Keay, L. 1970 Purification and characterization of the amylase ofBacillus subtilis NRRL B3411.Biotechnology and Bioengineering12, 251–271.
Priest, F.G. 1984 Extracellular Enzymes. InAspects of Microbiology, Vol. 9, eds Cole, J.A., Knowles, C.J. & Schlessinger, D. pp. 32–34. Wokingham, UK: Van Nostrand Reinhold.
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NCL Communication No. 5209.
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Khire, J.M., Pant, A. Thermostable, salt-tolerant amylase fromBacillus sp. 64. World J Microbiol Biotechnol 8, 167–170 (1992). https://doi.org/10.1007/BF01195840
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DOI: https://doi.org/10.1007/BF01195840