Abstract
Immunocytochemical staining based on the peroxidase-antiperoxidase method has shown that some neurosecretory cells (NSC) in the synganglion of the adult female tickOrnithodoros parkeri react with an antibody to bovine insulin. There are 18 regions of paraldehyde fuchsin-positive NSC of which three regions showed specific insulin-like immunoreactivity: anterolateral cheliceral, anteromedial stomodeal and posterior opisthosomal. Immunoreactivity can also be found in the extracellular surface of the neurilemma of the synganglion. This suggests a possible neurohemal site and release of neurohormone in a diffuse manner.
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References
Aeschlimann, A., 1968. La ponte chezOrnithodoros moubata Murray (Ixodoidea: Argasidae). Rev. Suisse Zool., 75: 1033–1039.
Binnington, K.C., 1987. Histology and ultrastructure of the acarine synganglion. In: A.P. Gupta (Editor), Arthropod Brain: Its Evolution, Development, Structure, and Functions, John Wiley and Sons, New York, NY, pp. 95–109.
Binnington, K.C. and Obenchain, F.D., 1983. Structure and function of the circulatory, nervous and neuroendocrine systems of ticks. In: F.D. Obenchain and R. Galun (Editors), Physiology of Ticks. Pergamon Press, Oxford, pp. 351–399.
Chinzei, Y. and Taylor, D., 1990. Regulation of vitellogenesis induction by engorgement in the soft tick,Ornithodoros moubata. In: M. Hoshi and O. Yamashita (Editors), Advances in Invertebrate Reproduction 5. Elsevier, Amsterdam, pp. 565–570.
Connat, J.-L., Ducommun, J.D., Diehl, P.A. and Aeschlimann, A., 1986. Some aspects of the control of the gonotrophic cycle in the tickOrnithodoros moubata (Ixodoidea, Argasidae). In: J.R. Sauer and J.A. Hair (Editors), Morphology, Physiology and Behavioral Biology of Ticks. Ellis Horwood, Chichester, UK, pp. 194–216.
Davis, K.T. and Shearn, A., 1977. In vitro growth of imaginal discs fromDrosophila melanogaster, Science, 196: 438–439.
Dhanda, V., 1967. Changes in neurosceretory activity at different stages in the adultHyalomma dromedarii Koch 1944. Nature, 214: 508–509.
Eisen, V., Warburg, M.R. and Galun, R., 1973. Neurosecretory activity as related to feeding and oogenesis in the fowl tickArgas persicus (Oken.). Gen. Comp. Endocrinol., 21: 331–340.
Gabbay, S. and Warburg, M.R. 1977. The diversity of neurosecretory cell types in the cave tickOrnithodoros tholozani. J. Morphol., 153: 371–386.
Girardie, A., Girardie, J., Proux, J., Remy, C. and Vieillemaringe, J., 1984. Neurosecretion in insects: Multisynthesis, mode of action, and multiregulation. In: J. Hoffmann and M. Porchet (Editors), Biosynthesis, Metabolism and Mode of Action of Invertebrate Hormones. Springer, Berlin, pp. 97–117.
Holman, G.M., Nachman, R.J. and Wright, M.S., 1990. Insect neuropeptides. Annu. Rev. Entomol., 35: 201–217.
Ioffe, I.D., 1964. Distribution of neurosecretory cells in the central nervous system ofDermacentor pictus Herm. (Chelicerata: Acarina). Dokl. Akad. Nauk SSSR Biol. Sci. Sect., 154: 229–232 (in English NAMRU-3 Transl. T326).
Iwami, M., Kawakami, A., Ishizaki, H., Takahasi, S.Y., Adachi, T., Suzuki, Y., Nagasawa, H. and Suzuki, A., 1989. Cloning of a gene encoding bombyxin, an insulin-like brain secretory peptide of the silkmothBombyx mori with prothoracicotropic activity. Dev. Growth Differ., 31: 31–37.
Joosse, J., 1984. Recent progress in endocrinology of molluses. In: J. Hoffmann and M. Porchet (Editors), Biosynthesis, Metabolism and Mode of Action of Invertebrate Hormones. Springer, Berlin, pp. 19–35.
Kanost, M.R., Kawooya, J.K., Law, J.H., Ryan, R.O., Heusden, C.V. and Ziegler, R., 1990. Insect haemolymph proteins. Adv. Insect Physiol., 22: 299–396.
Kramer, K.J., 1985. Vertebrate hormones in insects. In: G.A. Kerkut and L.I. Gilbert (Editors), Comparative Insect Physiology and Pharmacology, Vol. 7. Pergamon, Oxford, pp. 511–536.
Marzouk, A.S., Abdel-Moez, M.K. and Darwish, Z.E.A., 1987a. The effect of feeding and mating on the neurosecretory activity in femaleHyalomma dromedarii synganglion (Acari: Ixodoidea: Ixodidae). I. Changes in neurosecretory cell types in virgin and mated semifed females. J. Egypt. Soc. Parasitol., 17: 547–568.
Marzouk, A.S., Khalil, G.M., El-Tunsy, M. and Darwish, Z.E.A., 1987b. The effect of feeding and mating on the neurosecretory activity in femaleHyalomma dromedarii synganglion (Acari: Ixodoidea: Ixodidae). II. Changes in neurosecretory cell types in fully engorged females. J. Egypt. Soc. Parasitol., 17: 657–668.
Mosna, G., 1981. Insulin can completely replace serum inDrosophila melanogaster cell cultures in vitro. Experientia, 37: 466–567.
Oliver, J.H., Jr., Zhu, X.X., Vogel, G.N. and Dotson, E.M., 1991. Role of synganglion on oogenesis of the tickOrnithodoros parkeri (Acari: Argasidae). J. Parasitol. (in press).
Pound, J.M. and Oliver, J.H., Jr., 1982. Synganglial and neurosecretory morphology of femaleOrnithodoros parkeri (Cooley) (Acari: Argasidae). J. Morphol., 173: 159–177.
Shanbaky, N.M. and Khalil, G.M., 1975. The subgenusPersicargas (Ixodoidea: Argasidae:Argas). 22. The effect of feeding on hormonal control of egg development inArgas (Persicargas) arboreus. Exp. Parasitol., 37: 361–366.
Shanbaky, N.M., El-Said, A. and Helmy, N., 1990a. Changes in neurosecretory cell activity in femaleArgas (Argas) hermanni (Acari: Argasidae). J. Med. Entomol., 27: 975–981.
Shanbaky, N.M., Mansour, M.M., Main, A.J., El-Said, A. and Helmy, N., 1990b. Hormonal control of vitellogenesis inArgas (Argas) hermanni (Acari: Argasidae). J. Med. Entomol., 27: 968–974.
Sternberger, L.A., 1979. Immunocytochemistry, John Wiley and Sons, New York, NY, 354 pp.
Thorpe, A. and Duve, H., 1988. Insulin found at last? Nature, 331: 483–484.
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Zhu, X.X., Oliver, J.H. Immunocytochemical localization of an insulin-like substance in the synganglion of the tickOrnithodoros parkeri (Acari: Argasidae). Exp Appl Acarol 13, 153–159 (1991). https://doi.org/10.1007/BF01193666
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DOI: https://doi.org/10.1007/BF01193666