Skip to main content
SpringerLink
Log in

Localization of GABA-like immunoreactivity in the ectostriatum of domestic chicks: GABA immunocytochemistry combined with Golgi impregnation

  • Published:
Journal of Neurocytology

Summary

The distribution of GABA-like immunoreactivity (GABA-LI) in the ectostriatal core (Ec) of domestic chicks (one to two days old) was investigated using (1) preembedding GABA immunocytochemistry and (2) Golgi impregnation and gold-toning combined with postembedding GABA immunocytochemistry. Two major classes of neurons which display GABA-LI were identified in chick Ec. Firstly, large GABA immunopositive cells which comprise at least two further subtypes: an ovoid or polygonal form of 14–18 μm diameter with no apparent polarity of dendrites and a smaller cell (10–14 (μm) with ovoid or basket-shaped soma and often more polarized dendritic ramification. In both subtypes the dendritic surface is smooth or sparsely spiny. Secondly, a small GABA immunopositive cell which is characterized by a round cell body of 5–8 μm diameter and thin and sparsely ramifying dendrites of smooth surface or with irregular protrusions. Based upon comprehensive descriptions of ectostriatal cytoarchitectonics (Tömböletal., 1988c), and synaptology (Watanabeet al., 1985), we argue that the GABA-immunopositive cell types of chick Ec are likely to represent inhibitory interneurons comparable with GABAergic inhibitory cell types described in mammalian visual cortex.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ariens Kappers, C. U. (1921)Die Vergleichende Anatomie des Nervensystems der Wirbeltiere und des Menschen. Haarlem: E. F. Bohn.

    Google Scholar 

  • Barth, R. &Felix, D. (1974) Influence of GABA and glycine and their antagonists on inhibitory mechanisms of pigeon optic tectums.Brain Research 80, 532–7.

    Google Scholar 

  • Benowitz, L. I. (1980) Functional organization of the avian telencephalon. InComparative Neurology of the Telencepha-Ion (edited byEbbesson, O.), pp. 389–421. New York and London: Plenum.

    Google Scholar 

  • Benowitz, L. I. &Karten, H. J. (1976) Organization of the tectofugal visual pathway in the pigeon: A retrograde transport study.Journal of Comparative Neurology 167, 503–20.

    Google Scholar 

  • Brandon, C. (1985) Retinal GABA neurons: localization in vertebrate species using an antiserum to rabbit brain glutamate decarboxylase.Brain Research 344, 286–95.

    Google Scholar 

  • Bumm, A. (1883) Das Grosshirn der Vögel.Zeitschrift, fur Wissenschaftliche Zoologie 38, 430–67.

    Google Scholar 

  • Cherkin, A. (1969) Kinetics of memory consolidation: role of amnestic treatment parameters.Proceedings of the National Academy of Sciences (USA) 63, 1094–101.

    Google Scholar 

  • Csillag, A., Stewart, M. G., Curtis, E. M. &Rose, S. P. R. (1986) GABAergic structures in the chick forebrain. An autoradiographic study combined with GABA-immunocytochemistry.Society for Neuroscience Abstracts 12, 443 (Abstract).

    Google Scholar 

  • Csillag, A., Stewart, M. G. &Curtis, E. M. (1987) GABAergic structures in the chick telencephalon: GABA immunocytochemistry combined with light and electron microscope autoradiography, and Golgi impregnation.Brain Research 437, 283–97.

    Google Scholar 

  • Curtis, E. M. &Stewart, M. G. (1986) Development of γ-aminobutyric acid immunoreactivity in chick hyper-striatum ventrale and cerebellum: light and electron microscopical observations.Developmental Brain Research 30, 189–99.

    Google Scholar 

  • Domenici, L., Waldvogel, H. J., Matute, C. &Streit, P. (1988) Distribution of GABA-like immunoreactivity in the pigeon brain.Neuroscience 25, 931–50.

    Google Scholar 

  • Edinger, L., Wallenberg, A. &Holmes, G. (1903) Untersuchungen über die vergleichende Anatomie des Gehirns, 5. Das Vorderhirn der Vögel.Abhandlungen Senckenbergschen Naturforschung Gesellschaft 20, 343–426.

    Google Scholar 

  • Fairen, A., Peters, A. &Saldanha, J. (1977) A new procedure for examining Golgi impregnated neurons by light and electron microscopy.Journal of Neurocytology 6, 311–37.

    Google Scholar 

  • Freund, T. F., Martin, K. A. C., Smith, A. D. &Somogyi, P. (1983) Glutamate decarboxylase-immunoreactive terminals of Golgi impregnated axoaxonic cells and of presumed basket cells in synaptic contact with pyramidal neurons of the cat's visual cortex.Journal of Comparative Neurology 221, 263–78.

    Google Scholar 

  • Gabbott, P., Somogyi, J., Stewart, M. G. &Hámori, J. (1986) GABA-immunoreactive neurons in the rat cerebellum: a light and electron microscope study.Journal of Comparative Neurology 251, 474–90.

    Google Scholar 

  • Hall, K., Brauth, S. E. &Kitt, C. A. (1984) Retrograde transport of3 HGABA in the striatotegmental system of the pigeon.Brain Research 310, 157–63.

    Google Scholar 

  • Henke, H. &Fonnum, F. (1976) Topographical and sub-cellular distribution of choline acetyltransferase and glutamate decarboxylase in pigeon optic tectum.Journal of Neurochemistry 27, 387–91.

    Google Scholar 

  • Hodgson, A. J., Penke, B., Erdei, A., Chubb, I. W. & Somogyi, P. (1985) Antiserum to γ-aminobutyric acid. I. Production and characterisation using a new model system.Journal of Histochemistry and Cytochemistry 33, 229–39.

    Google Scholar 

  • Hodos, W. &Karten, H. J. (1970) Visual intensity and pattern discrimination deficits after lesions of ectostri-atum in pigeons.Journal of Comparative Neurology 140, 53–68.

    Google Scholar 

  • Holländer, H. &Vanegas, H. (1981) Identification of pericellular baskets in the cat striate cortex: Light and electron microscopic observations after uptake of horseradish peroxidase.Journal of Neurocytology 10, 577–87.

    Google Scholar 

  • Horn, G. (1985)Memory, Imprinting and the Brain: An Inquiry into Mechanisms. Oxford, Clarendon.

    Google Scholar 

  • Hunt, S. P. &Künzle, H. (1976) Selective uptake and transport of label within three identified neuronal systems after injection of3H-GABA into the pigeon optic tectum: An autoradiographic and Golgi study.Journal of Comparative Neurology 170, 173–90.

    Google Scholar 

  • Jalilian Tehrani, M. H. &Barnes, E. M., Jr. (1986) Ontogeny of the GABA receptor complex in chick brain: studiesin vivo andin vitro.Developmental Brain Research 25, 91–8.

    Google Scholar 

  • Jong, Y.-J., Thampy, K. G. &Barnes, E. M., Jr. (1986) Ontogeny of GABAergic neurons in chick brain: studiesin vivo andin vitro.Developmental Brain Research 25, 83–90.

    Google Scholar 

  • Källén, B. (1962) Embryogenesis of brain nuclei in the chick telencephalon.Ergebnisse der Anatomie und Entwieck lungsgeschichte 36, 62–82.

    Google Scholar 

  • Karten, H. J. &Hodos, W. (1970) Telencephalic projections of the nucleus rotundus in the pigeon (Columba livia).Journal of Comparative Neurology 140, 35–52.

    Google Scholar 

  • Müller, C. M. (1987) γ-Aminobutyric acid immunoreactivity in brainstem auditory nuclei of the chicken.Neuro-science Letters 77, 272–6.

    Google Scholar 

  • Müller, C. M. &Scheich, H. (1987) GABAergic inhibition increases the neuronal selectivity to natural sounds in the avian auditory forebrain.Brain Research 414, 376–80.

    Google Scholar 

  • Nixdorf, B. E. &Bischof, H. -J. (1982) Afferent connections of the ectostriatum and visual Wulst in the zebra finch (Taeniopygia guttata castanotis Gould) — an HRP study.Brain Research 248, 9–17.

    Google Scholar 

  • Revzin, A. M. &Karten, H. J. (1966/67) Rostral projections of the optic tectum and the nucleus rotundus in the pigeon.Brain Research 5, 264–76.

    Google Scholar 

  • Ribak, C. E. (1978) Aspinous and sparsely-spinous stellate neurons in the visual cortex of rats contain glutamic acid decarboxylase.Journal of Neurocytology 7, 461–78.

    Google Scholar 

  • Rose, M. (1914) Uber die cytoarchitektonishe Gliederung des Vorderhirns der Vögel.Journal fur Psychologie und Neurologie, Leipzig 21, 278–352.

    Google Scholar 

  • Rose, S. P. R. (1985) Passive avoidance training in the chick: a model for the analysis of the cell biology of memory storage. InNeural Mechanism of Conditioning (edited byAlkon, D. L. &Woody, C. D.), pp. 232–48. New York: Plenum.

    Google Scholar 

  • Somogyi, P. &Cowey, A. (1981) Combined Golgi and electron microscopic study on the synapses formed by double bouquet cells in the visual cortex of the cat and the monkey.Journal of Comparative Neurology 195, 547–66.

    Google Scholar 

  • Somogyi, P. &Hodgson, A. J. (1985) Antiserum to γ-aminobutyric acid. III. Demonstration of GABA in Golgi-impregnated neurons and in conventional electron microscopic sections of cat striate cortex.Journal of Histochemistry and Cytochemistry 33, 249–57.

    Google Scholar 

  • Somogyi, P., Hodgson, A. J., Chubb, I. W., Penke, B. &Erdei, A. (1985) Antiserum to gamma-aminobutyric acid. II. Immunocytochemical application to the central nervous system.Journal of Histochemistry and Cytochemistry 33, 240–8.

    Google Scholar 

  • Stewart, M. G., Bourne, R. C., Chmielowska, J., Kálmán, M., Csillag, A. &Stanford, D. (1988) Quantitative autoradiographic analysis of the distribution of3 Hmuscimol binding to GABA receptors in chick brain.Brain Research 456, 387–39.

    Google Scholar 

  • Tömböl, T., Csillag, A., Tömböl, T., Csillag, A. &Stewart, M. G. (1988a) Cell types of the hyperstriatum ventrale of the domestic chicken (Gallus domesticus): A Golgi study.Journal für Hirnforschung 29 (3), 319–34.

    Google Scholar 

  • Tömböl, T., Csillag, A. &Stewart, M. G. (1988b) Cell types of the paleostriatal complex of the domestic chicken (Gallus domesticus): A Golgi study.Journal für Hirnforschung 29 (5) 493–507.

    Google Scholar 

  • Tömböl, T., Maglóczky, Zs., Stewart, M. G. &Csillag, A. (1988c) The structure of chicken ectostriatum. I. Golgi study.Journal für Hirnforschung 29 (5) 525–46.

    Google Scholar 

  • Watanabe, M., Ito, H. &Ikushima, M. (1985) Cyto-architecture and ultrastructure of the avian ectostriatum: afferent terminals from the dorsal telencephalon and some nuclei in the thalamus.Journal of Comparative Neurology 236, 241–57.

    Google Scholar 

  • Watson, A. H. D., Mccabe, B. J. &Horn, G. (1988) Ultrastructural irnmunocytochemistry of γ-aminobutyric acid (GABA) in the chick hyperstriatum ventrale.Neuroscience Letters Supplement 32, S 86 (Abstract).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. 1st Department of Anatomy, Semmelweis University Medical School, Budapest, Hungary

    A. Csillag & Teréz Tömböl

  2. Brain and Behaviour Research Group, The Open University, Milton Keynes, UK

    R. C. Bourne, Sanjay N. Patel & Michael G. Stewart

Authors
  1. A. Csillag
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. C. Bourne
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sanjay N. Patel
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Michael G. Stewart
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Teréz Tömböl
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Csillag, A., Bourne, R.C., Patel, S.N. et al. Localization of GABA-like immunoreactivity in the ectostriatum of domestic chicks: GABA immunocytochemistry combined with Golgi impregnation. J Neurocytol 18, 369–379 (1989). https://doi.org/10.1007/BF01190840

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01190840

Keywords

Search

Navigation