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Myelin formation following transplantation of normal fetal glia into myelin-deficient rat spinal cord

  • Published:
Journal of Neurocytology

Summary

Structurally normal myelin sheaths develop in the spinal cord of juvenile myelin-deficient rats (mdr) 11 days after transplantation of normal fetal spinal cord fragments or cultured cells that do not yet express galactocerebroside. Cultures result in more extensive myelin formation, and in both cases the myelin that forms is located primarily at or near the site of transplantation.

Myelin formation also occurs after transplantation of postnatal donor tissue, but the extent diminishes with donor age, and none was seen after transplantation of adult donor tissue over the two-week period studied. Injection of killed tissue, tissue derived from mouse donors or an extract of myelin also did not lead to myelin formation. The results imply that myelin formed in the host following transplantation was generated by oligodendrocytes newly differentiated from donor precursor cells rather than by donor oligodendrocytes that were already mature at the time of transplantation or by host oligodendrocytes that took up components of the injected material.

We conclude that exogenous fetal glial cell precursors are able to survive, differentiate and form myelin in the environment of the juvenile mdr spinal cord.

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References

  • Abney, E. R., Bartlett, P. P. &Raff, M. C. (1981) Astrocytes, ependymal cells, and oligodendrocytes develop on schedule in dissociated cultures of embryonic rat brain.Developmental Biology 83, 301–10.

    Google Scholar 

  • Aguayo, A. J., Bray, G. M., Perkins, C. S. &Duncan, I. D. (1978) Axon-sheath interactions in peripheral and central nervous system transplants. InSociety for Neuroscience Symposia (edited byFerrendelli, J. A.) Vol. 4, pp. 361–83. Bethesda: Society for Neuroscience.

    Google Scholar 

  • Baulac, M. F., Lachapelle, F., Gout, O., Berger, B., Baumann, N. &Gumpel, M. (1987) Transplantation of oligodendrocytes in the newborn mouse brain: extension of myelination by transplanted cells. Anatomical study.Brain Research 420, 39–47.

    Google Scholar 

  • Billings-Gagliardi, S., Adcock, L. H., Lamperti, E. D., Schwing-Stanhope, G. &Wolf, M. K. (1983) Myelination of jp, jpmsd, and qk axons by normal glia in vitro: ultrastructural and autoradiographic evidence.Brain Research 268, 255–66.

    Google Scholar 

  • Bunge, M. B., Bunge, R. P. &Ris, H. (1961) Ultrastructural study of remyelination in an experimental lesion in adult cat spinal cord.Journal of Biophysical and Biochemical Cytology 10, 67–94.

    Google Scholar 

  • Buse, E., Kindler-Rörborn, A. &Rajewsky, M. F. (1989)In vitro experiments on neuronal and glial cell lineages among the ventricular cells of the mouse neural plate.International Journal of Developmental Neuroscience 7, 103–13.

    Google Scholar 

  • Del Cerro, M., Notier, M. F., Wiegand, S., Qi Jiang, L. &Del Cerro, C. (1988) Intraretinal transplantation of fluorescently labelled retinal cell suspensions.Anatomical Record 220, 29A-30A.

    Google Scholar 

  • Dentinger, M. P., Barron, K. D. &Csiza, C. K. (1982) Ultrastructure of the CNS in a myelin-deficient rat.Journal of Neurocytology 11, 671–91.

    Google Scholar 

  • Duncan, I. D., Hammang, J. P. &Gilmore, S. A. (1988a) Schwann cell myelination of the myelin deficient rat spinal cord following X-irradiation.Glia 1, 233–39.

    Google Scholar 

  • Duncan, I. D., Hammang, J. P., Jackson, K. F., Wood, P. M., Bunge, R. P. &Langford, L. (1988b) Transplantation of oligodendrocytes and Schwann cells into the spinal cord of the myelin-deficient rat.Journal of Neurocytology 17, 351–60.

    Google Scholar 

  • Friedman, E., Nilaver, G., Carmel, P., Perlow, M., Spatz, L. &Latov, N. (1986) Myelination by transplanted fetal and neonatal oligodendrocytes in a dysmyelinating mutant.Brain Research 378, 142–6.

    Google Scholar 

  • Gansmuller, A., Lachapelle, F., Baron-Van Evercooren, A., Hauw, J. J., Baumann, N. &Gumpel, M. F. (1986) Transplantation of newborn CNS fragments into the brain of shiverer mutant mice: extensive myelination by transplanted oligodendrocytes. II. Electron microscopic study.Developmental Neuroscience 8, 197–207.

    Google Scholar 

  • Gout, O., Gansmuller, A., Baumann, N. &Gumpel, M. (1988) Remyelination by transplanted oligodendrocytes of a demyelinated lesion in the spinal cord of the adult shiverer mouse.Neuroscience Letters 87, 195–99.

    Google Scholar 

  • Gumpel, M., Baumann, N., Raoul, M. &Jacque, C. (1983) Survival and differentiation of oligodendrocytes from neural tissue transplanted into newborn mouse brain.Neuroscience Letters 37, 307–11.

    Google Scholar 

  • Gumpel, M., Lachapelle, F., Gansmuller, A., Baulac, M., Baron-Van Evercooren, A. &Baumann, N. (1987) Transplantation of human embryonic oligodendrocytes into shiverer brain.Annals of the New York Academy of Sciences 495, 71–85.

    Google Scholar 

  • Hasegawa, M. &Rosenbluth, J. (1990) Transplantation of labelled fetal spinal cord fragments into md rat.Anatomical Record 228, in press.

  • Inoue, H. &Kirschner, D. A. (1984) New X-ray spacings from central myelinated tissue.Journal of Neurocytology 13, 883–94.

    Google Scholar 

  • Kohsaka, S., Yoshida, K., Inoue, Y., Shinozaki, T., Takamaya, H., Inoue, H., Mikoshiba, K., Takamatsu, K., Otani, M., Toya, S. &Tsukada, Y. (1986) Transplantation of bulk separated oligodendrocytes into the brain of shiverer mutant mice: immunohistochemical and electron microscopic studies on myelination.Brain Research 372, 137–42.

    Google Scholar 

  • Lachapelle, F., Gumpel, M., Baulac, M., Jacque, C., Due, P. &Baumann, N. (1984) Transplantation of CNS fragments into the brain of shiverer mice: extensive myelination by implanted oligodendrocytes. I. Immunohistochemical studies.Developmental Neuroscience 6, 325–34.

    Google Scholar 

  • Lees, M. B. &Sakura, J. D. (1978) Preparation of proteolipids. InResearch Methods in Neurochemistry (edited byMarks, R. &Rodnight, R.) Vol. 4, pp. 345–69. New York: Plenum.

    Google Scholar 

  • Mcdonald, W. I. (1974) Remyelination in relation to clinical lesions in the CNS.British Medical Bulletin 30, 186–9.

    Google Scholar 

  • Peters, A. (1968) The morphology of axons of the central nervous system. InThe Structure and Function of Nervous Tissue (edited byBourne, G. H.) Vol. 1, pp. 141–86. New York: Academic Press.

    Google Scholar 

  • Peters, A., Palay, S. L. &Webster, H. deF. (1976)The fine structure of the nervous system: The neurons and supporting cells, Philadelphia: W. B. Saunders Company.

    Google Scholar 

  • Poltorac, M. &Freed, W. J. (1989) Immunological reactions induced by intracerebral transplantation: evidence that host microglia but not astroglia are the antigen-presenting cells.Experimental Neurology 103, 222–33.

    Google Scholar 

  • Prineas, J. W. (1985) The neuropathology of multiple sclerosis. InHandbook of Clinical Neurology (edited byKoetsier, J. C.) Demyelinating Diseases, Vol. 3(47), pp. 213–57. Amsterdam: Elsevier.

    Google Scholar 

  • Raine, C. S. (1978) Pathology of demyelination. InPhysiology and Pathobiology of Axons (edited byWaxman, S. G.) pp. 283–311. New York: Raven Press.

    Google Scholar 

  • Raine, C. S., Scheinberg, L. &Waltz, J. M. (1981) Multiple sclerosis. Oligodendrocyte survival and proliferation in an active established lesion.Laboratory Investigation 45, 534–46.

    Google Scholar 

  • Rosen, C. L., Bunge, R. P., Ard, M. D. &Wood, P. M. (1989) Type 1 astrocytes inhibit myelination by adult rat oligodendrocytes in vitro.Journal of Neuroscience 9, 3371–9.

    Google Scholar 

  • Rosenbluth, J. (1987) Abnormal axolial junctions in the myelin-deficient rat mutant.Journal of Neurocytology 16, 497–509.

    Google Scholar 

  • Rosenbluth, J. (1988) Role of glial cells in the differentiation and function of myelinated axons.International Journal of Developmental Neuroscience 6, 3–24.

    Google Scholar 

  • Rosenbluth, J., Hasegawa, M. &Schiff, R. (1988) Do astrocytes cause the myelin deficiency in the mdr mutant?Society for Neuroscience Abstracts 14, 118.

    Google Scholar 

  • Rosenbluth, J., Hasegawa, M. &Schiff, R. (1989a) Myelin-formation in myelin-deficient rat spinal cord following transplantation of normal fetal spinal cord.Neuroscience Letters 97, 35–40.

    Google Scholar 

  • Rosenbluth, J., Hasegawa, M., Shirasaki, N., Rosen, C. L. &Liu, Z. (1989b) Myelin formation in myelin-deficient rat spinal cord after transplantation of normal fetal spinal cord cultures.Society for Neuroscience Abstracts 15, 10.

    Google Scholar 

  • Sasaki, M. &Ide, C. (1989) Demyelination and remyelination in the dorsal funiculus of the rat spinal cord after heat injury.Journal of Neurocytology 18, 225–39.

    Google Scholar 

  • Streilein, J. W. (1988) Transplantation immunobiology in relation to neural grafting: Lessons learned from immunlogic privilege in the eye.International Journal of Developmental Neuroscience 6, 497–511.

    Google Scholar 

  • Wolf, M. K., Schwing, G. B., Adcock, L. H. &Billingsgagliardi, S. (1981) Hypomyelinated mutant mice. III. Increased myelination in mutant cerebellum co-cultured with normal optic nerve.Brain Research 206, 193–7.

    Google Scholar 

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Authors and Affiliations

  1. Departments of Physiology and Rehabilitation Medicine, New York University School of Medicine, 10016, New York, NY, USA

    J. Rosenbluth, M. Hasegawa, N. Shirasaki, C. L. Rosen & Z. Liu

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  1. J. Rosenbluth
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  2. M. Hasegawa
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  3. N. Shirasaki
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  4. C. L. Rosen
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  5. Z. Liu
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Rosenbluth, J., Hasegawa, M., Shirasaki, N. et al. Myelin formation following transplantation of normal fetal glia into myelin-deficient rat spinal cord. J Neurocytol 19, 718–730 (1990). https://doi.org/10.1007/BF01188040

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