Summary
Anhydrotetracycline oxygenase was partially purified on a Sephadex G-25 column and DEAE-cellulose. The purified enzyme was inhibited by tetracycline derivatives added to the reaction mixture. Certain concentrations of calcium and magnesium ions stimulated the enzymic activity; further increase of the concentration resulted in a sharp decrease of the enzymic activity. The enzyme activity was also stimulated by Cu2+, Ni2+, Co2+, Fe3+ and Mn2+ present in suitable concentrations in the reaction mixture. Michaelis constant of the enzyme was found to be 2.2.10-5.
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Běhal, V., Neužil, J. & Hošťálek, Z. Effect of tetracycline derivatives and some cations on the activity of anhydrotetracycline oxygenase. Biotechnol Lett 5, 537–542 (1983). https://doi.org/10.1007/BF01184945
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DOI: https://doi.org/10.1007/BF01184945