Abstract
In the present study, some beneficial effects of mouse prolactin (PRL) on spermatozoa were demonstrated in mice. The motility rate of spermatozoa is well maintained during incubation in modified Krebs-Ringer bicarbonate solution (mKRB) containing PRL (10 or 100 ng/ml) for up to 120 min. When spermatozoa that had been preincubated in the same PRL-containing mKRB were incubated with oocytes in mKRB, significantly more spermatozoa attached to the zona pellucida of each oocyte. When spermatozoa that had been preincubated in mKRB with PRL (50 and 100 ng/ml) for only 15 or 30 min were used for in vitro fertilization (IVF), significantly higher fertilization rates were yielded by those spermatozoa than by control spermatozoa preincubated without PRL for the same periods. The prolongation of the preincubation period did not result in increased fertilization rates. Thus, PRL demonstrated biological effects on spermatozoa by shortening the optimal preincubation period for spermatozoa to acquire capacitation and by maintaining their motility and ability of the attachment to the oocyte during IVF. The results are relevant to clinical application of PRL for infertile patients with oligozoospermia or asthenozoospermia.
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References
Bohnet HG, Dahlen HG, Wuttke W, Schneider HPG: Hyperprolactinemic anovulatory syndrome. J Clin Endocrinol Metab 1976;42:132–143
Bartke A: Role of prolactin in reproduction in male mammals. Fed Proc 1980;39:2577–2581
Lopata A, Johnston IW, Hoult IJ, Speirs IP: Pregnancy following intrauterine implantation of an embryo obtained by in vitro fertilization of preovulatory egg. Fertil Steril 1980;33:117–121
Asch RH, Balmaceda JP, Ellsworth LR, Wang PC: Pregnancy after translaparoscopic gamete intrafallopian transfer. Lancet 1984;2:1034–1035
Gluckman PD, Ballard PL, Kaplan SL, Liggins GC, Grumbach MM: Prolactin in umbilical cord blood and the respiratory distress syndrome. J Pediat 1978;93:1011–1014
McNatty KP: Relationship between plasma prolactin and the endocrine microenvironment of the developing human antral follicle. Fertil Steril 1979;4:433–438
Laufer N, Botero-Ruiz W, DeCherney AH, Haseltine FP, Polan ML, Behrmann HR: Gonadotropin and prolactin levels in follicular fluid of human ova successfully fertilized in vitro. J Clin Endocrinol Metab 1984;58:430–434
Wramsby H, Kullander S, Liedholm P, Rannevik G, Sundstrom P, Thorell J: The success rate of in vitro fertilization of human oocytes in relation to the concentrations of different hormones in follicular fluid and peripheral plasma. Fertil Steril 1981;36:448–454
Rainthaller A, Deutinger J, Riss P, Muller-Tyl E, Fischl F, Janisch H: The influence of elevated serum prolactin on the endocrine response and fertilization rate of human oocytes in stimulated cycles. 42nd Annual Meeting of the American Fertility Society, Toronto, 1986, p 57 (abstr)
Forman R, Fishel SB, Edwards RG, Walters E: The influence of transient hyperprolactinemia on in vitro fertilization in humans. J Clin Endocrinol Metab 1985;60:517–522
Rainthaller A, Deutinger J, Riss P, Muller-Tyl E, Fischl F, Bieglmayer Ch, Janisch H: Relationship between the steroid and prolactin concentration in follicular fluid and the maturation and fertilization of human oocytes. J Vitro Fert Embryo Transfer 1987;4:228–231
Lee MS, Ben-Rafael Z, Meloni F, Mastroianni L Jr, Flickinger GL: Relationship of human oocyte maturity, fertilization, and cleavage to follicular fluid prolactin and steroids. J Vitro Fert Embryo Transfer 1987;4:168–172
Fukuda A, Noda Y, Mori T, Mori C, Hashimoto H, Hoshino K: The effects of prolactin on gametes and zygote during in vitro fertilization in the mouse. J Vitro Fert Embryo Transfer 1988;5:25–30
Mori C, Hashimoto H, Hoshino K, Fukuda A, Noda Y, Mori T: Influences of prolactin upon spermatogenesis and spermatozoa during in vitro fertilization in mice. J Vitro Fert Embryo Transfer 1988;5:61–66
Hoshino K: Biological effects of prolactin on spermatozoa, oocyte and fertilization.In Prolactin Gene Family and Its Receptors—Molecular Biology to Clinical Problems, Excerpta Medica International Congress Series, K Hoshino (ed) Amsterdam, Elsevier, 1988 (in press)
Kohmoto K: Mouse prolactin obtained by pituitary organ culture in a serum free medium. Endocrinol Jap 1975;5:465–469
Fukuda A, Noda Y, Matsumoto H, Tsukui S, Yano J, Mori T: The influences of water quality on in vitro fertilization and embryo development for the mouse. J Vitro Fertil Embryo Transfer 1987;5:25–30
Kasai K, Minato Y, Toyoda Y: Fertilization and development in vitro of mouse eggs from inbred strain and F1 hybrids. Jpn J Anim Reprod 1978;24:19–22
Toyoda Y, Yokoyama M, Hoshi T: Studies on the fertilization of mouse eggs in vitro. Jpn J Anim Reprod 1971;16:147–157
Miyamoto H, Chang MC: The importance of serum albumin and metabolic intermediates for capacitation of spermatozoa and for fertilization of mouse eggs in vitro. J Reprod Fert 1973;32:193–205
Mori C, Harigaya T, Hoshino K, Fukuda A: Effect of prolactin on acrosomal reaction of spermatozoa in mice in vitro.In Prolactin Gene Family and Its Receptors-Molecular Biology to Clinical Problems, Excerpta Medica International Congress Series, K Hoshino (ed). Amsterdam, Elsevier, 1988 (in press)
Mori C, Sakai S, Harigaya T, Hoshino K: Immunohistochemical localization of prolactin receptor in the liver, suprarenal glands, ovaries and spermatozoa in rats. Proceedings, 5th International Congress on Prolactin, 1988, p 119–8c
Hashimoto H, Izumi S, Mori C, Nakane PK, Hoshino K: Immunohistochemical observation of prolactin receptor in rat epididymal spermatozoa by backscattered electron imaging.In Prolactin Gene Family and Its Receptors-Molecular Biology to Clinical Problems, Excerpta Medica International Congress Series, K Hoshino (ed). Amsterdam, Elsevier, 1988 (in press)
Mori C, Hashimoto H, Harigaya T, Hoshino K: Biological effects of prolactin on spermatozoa. J Fertil Implant (Tokyo) 1988;6 (in press)
Shah GV, Desal RB, Sheth AR: Effect of prolactin on metabolism on human spermatozoa. Fertil Steril 1976;27:1292–1294
Sheth AR, Vaidya RA, Raiker RS: Presence of prolactin in human cervical mucus. Fertil Steril 1976;27:397–398
Overstreet JW, Gould JE, Katz DF, Hanson FW: In vitro capacitation of human spermatozoa after passage through a column of cervical mucus. Fertil Steril 1980;34:604–606
Gould JE, Overstreet JW, Hanson FW: Assessment of human sperm function after recovery from female reproductive tract. Biol Reprod 1984;31:888–894
Lambert H, Overstreet JW, Morales P, Hanson FW, Yanagimachi R: Sperm capacitation in the human female reproductive tract. Fertil Steril 1985;44:325–327
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Fukuda, A., Mori, C., Hashimoto, H. et al. Effects of prolactin during preincubation of mouse spermatozoa on fertilizing capacity in vitro. J Assist Reprod Genet 6, 92–97 (1989). https://doi.org/10.1007/BF01130733
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DOI: https://doi.org/10.1007/BF01130733