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A rapid and efficient method to enrich SAF-protein from scrapie brains of hamsters

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Bioscience Reports

Abstract

Scrapie hamster brains contain at least 5–10 μg of scrapie-associated fibrils (SAF) per brain as estimated by the amount of its major constituent, a protein of about 26 000 daltons (SAF-protein). It can be extracted efficiently by a 10% solution of sarkosyl and can be enriched by differentia] centrifugation and buffer extraction. Scrapie infectivity, SAF, and SAF-protein copurify.

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Authors and Affiliations

  1. Robert Koch-Institut des Bundesgesundheitsamtes, Nordufer 20, D-1000, Berlin 65, Germany

    Horst Hilmert & Heino Diringer

Authors
  1. Horst Hilmert
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  2. Heino Diringer
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Hilmert, H., Diringer, H. A rapid and efficient method to enrich SAF-protein from scrapie brains of hamsters. Biosci Rep 4, 165–170 (1984). https://doi.org/10.1007/BF01120313

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  • DOI: https://doi.org/10.1007/BF01120313

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