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Plasmid maintenance and gene expression of a recombinant culture under aerobic and anaerobic conditions

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Summary

The stability and gene expression of a batch culture ofEscherichia coli, strain C600, carrying the plasmid pKN401 under both aerobic and anaerobic conditions were studied. It was observed that the plasmid was stable under both conditions and during a step change in the environment in which oxygen and nitrogen were supplied alternately. In addition, the β-lactamase specific activity was sensitive to the availability of oxygen, with higher activities and a strong dependence on optical density observed under anaerobic conditions.

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Authors and Affiliations

  1. Department of Chemical Engineering, Rice University, 77251-1892, Houston, Texas, USA

    Gerard J. Tolentino & Ka-Yiu San

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  1. Gerard J. Tolentino
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  2. Ka-Yiu San
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Tolentino, G.J., San, KY. Plasmid maintenance and gene expression of a recombinant culture under aerobic and anaerobic conditions. Biotechnol Lett 10, 373–376 (1988). https://doi.org/10.1007/BF01087431

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  • DOI: https://doi.org/10.1007/BF01087431

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