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Molecular phylogenetics ofCochlearia (Brassicaceae) and allied genera based on nuclear ribosomal ITS DNA sequence analysis contradict traditional concepts of their evolutionary relationship

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Abstract

The systematics and phylogeny of the genusCochlearia and allied genera are unsettled. There are no clearly defined genera and subtribal structures to determine subtribeCochleariinae in respect to subtribeThlaspidinae. The use of morphological data, such as fruit form or embryo characters have resulted in contradictory taxonomic concepts in the past due to their homoplastic nature. We investigated all sections of genusCochlearia recognised in the most common concepts, as well as some genera such asIonopsidium, Bivonaea, Pastorea andThlaspi s. l. pro parte. Previous studies based on molecular data and morphological studies have shown close relationships between taxa from subtribeCochleariinae andThlaspidinae. The Internal Transcribed Spacer regions of the nuclear encoded ribosomal DNA operon and the plastidictrnL intron were sequenced from a number of genera. A molecular phylogeny was derived and compared to traditional classification systems. These data grouped sections ofCochlearia outside theCochlearial Ionopsidium core group and integrated them either closely to genusNoccaea in subtribeThlaspidinae (sect.Pseudosempervivum) or positioned them outside both theCochlearia core group and theThlaspi s. l. clade (sect.Hilliella). The molecular data indicate that subtribal arrangements in tribeLepidieae are artificial and do not reflect evolutionary history. The genusCochlearia is represented by sectionsCochlearia andGlaucocochlearia and the genusIonopsidium should be integrated intoCochlearia.

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References

  • Avetisian, V. E., 1983: The system of the familyBrassicaceae. — Bot. Zhurn. (Moscow & Leningrad)68: 1297–1305.

    Google Scholar 

  • Baldwin, B. G., Sanderson, M. J., Porter, J. M., Wojciechowski, M. F., Campbell, C. S., Donoghue, M. J., 1995: The ITS region of nuclear ribosomal DNA: a valuable source of evidence on angiosperm phylogeny. — Ann. Missouri Bot. Gard.82: 247–277.

    Google Scholar 

  • Battand, D., 1896:Ionopsidium. — Bull. Soc. Bot. France43: 259.

    Google Scholar 

  • Böhle, U., Hilger, H., Cerff, R., Martin, W. F., 1994: Non-coding chloroplast DNA for plant molecular systematics at the infrageneric level. — InSchierwater, B., Streit, B., Wagner, G. P., Desalle, R., (Eds): Molecular ecology and evolution: Approaches and applications, pp. 391–402. — Basel: Birkhäuser.

    Google Scholar 

  • Boissier, P. E., 1867: Flora Orientalis,1. — Basel, Geneva: H. Georg.

    Google Scholar 

  • Campell, C. S., Donoghue, M. J., Baldwin, B. G., Wojciechowski, M. F., 1995: Phylogenetic relationships inMaloideae (Rosaceae): evidence from sequences of the internal transcribed spacers of nuclear ribosomal DNA and its congruence with morphology. — Amer. J. Bot.82: 903–918.

    Google Scholar 

  • De Candolle, A. P., 1821: Mémoires sur la famille des Crucifères. — Mém. Mus. Hist. Nat.7: 169–252.

    Google Scholar 

  • Downie, S. R., Katz-Downie, D. S., 1996: A molecular phylogeny ofApiaceae subfamilyApioideae: evidenve from nuclear ribosomal DNA internal transcribed spacer sequences. — Amer. J. Bot.83: 234–251.

    Google Scholar 

  • Doyle, J. J., Doyle, J. L., 1987: A rapid DNA isolation procedure for small quantities of fresh leaf tissue. — Phytochem. Bull.19: 11–15.

    Google Scholar 

  • Dvorak, F., 1971: On the evolutionary relationships in the familyBrassicaceae. — Feddes Repert.82: 357–372.

    Google Scholar 

  • Endress, P. K., 1992: Evolution and floral diversity: the phylogenetic surrounding ofArabidopsis andAntirrhinum. — Int. J. Pl. Sci.153: 106–122.

    Google Scholar 

  • Felsenstein, J., 1985: Confidence limits on phylogenies: an approach using the bootstrap. — Evolution39: 783–791.

    Google Scholar 

  • Gielly, L., Taberlet, P., 1994: The use of chloroplast DNA to resolve plant phylogenies: noncoding versusrbcL sequences. — Molec. Biol. Evol.11: 769–777.

    Google Scholar 

  • Ham, R. C. H. J. van, Hart', H., Mes, T. H. M., Sandbrink, J. M., 1994: Molecular evolution of noncoding regions of the chloroplast genome in theCrassulaceae and related species. — Curr. Genet.25: 558–566.

    Google Scholar 

  • Hayek, A. von, 1911: Entwurf eines Cruciferen Systems auf phylogenetischer Grundlage. — Beih. Bot. Centralbl.27: 127–335.

    Google Scholar 

  • Hedge, I. C., 1976: A systematic and geographical survey of the old worldCruciferae. — InVaughan, J. G., MacLeod, A. J., Jones, M. B., (Eds): The biology and chemistry of theCruciferae, pp. 1–35. — London: Academic Press.

    Google Scholar 

  • Heywood, V. H., 1993:Ionopsidium. — InTutin, T. G., Burges, N. A., Chater, A. D., Edmondson, J. R., Heywood, V. H., Moore, D. M., Valentine, D. H., Walters, S. N., Webb, D. A., (Eds): Flora Europaea. 2nd edn,1, pp. 383. — Cambridge: Cambridge University Press.

    Google Scholar 

  • Janchen, E., 1942: Das System der Cruciferen. — Oesterr. Bot. Z.91: 1–21.

    Google Scholar 

  • Kim, K.-J., Jansen, R. K., 1994: Comparisons of phylogenetic hypotheses among different data sets in dwarf dandelions (Krigia, Asteraceae): additional information from internal transcribed spacer sequences of nuclear ribosomal DNA. — Pl. Syst. Evol.190: 157–185.

    Google Scholar 

  • Koch, M., Mummenhoff, K., Zunk, K., 1993: Isoelektrische Fokussierung der Untereinheiten der Rubisco inThlaspi (Brassicaceae): Weitere Hinweise auf eine Formengattung. — Feddes Repert.104: 371–381.

    Google Scholar 

  • —,Hurka, H., Mummenhoff, K., 1996: Chloroplast DNA restriction site variation and RAPD-analyses inCochlearia (Brassicaceae). Biosystematics and speciation. — Nordic J. Bot.16: 585–604.

    Google Scholar 

  • —,Mummenhoff, K., Hurka, H., 1998: Molecular biogeography and evolution ofMicrothlaspi perfoliatum s. l. polyploid complex (Brassicaceae): Chloroplast DNA and nuclear ribosomal DNA restriction site variation. — Canad. J. Bot.76: 382–396.

    Google Scholar 

  • Ma, Y.-C., Zhao, Y.-Z., 1979:Yinshania, a new genus of ChineseCruciferae. — Acta Phytotax. Sinica17: 113–114.

    Google Scholar 

  • Meyer, F. K. M., 1973: Conspectus der „Thlaspi“—Arten Europas, Afrikas und Vorderasiens. — Feddes Repert.84: 449–470.

    Google Scholar 

  • —, 1979: Kritische Revision der „Thlaspi“—Arten Europas, Afrikas und Vorderasiens. I. Geschichte, Morphologie und Chorologie. — Feddes Repert.90: 129–154.

    Google Scholar 

  • —, 1991: Seed-coat anatomy as a character for a new classification ofThlaspi. — Fl. Veg. Mundi 9: 9–15.

    Google Scholar 

  • Mummenhoff, K., Koch, M., 1994: Chloroplast restriction site variation and phylogenetic relationships in the genusThlaspi sensu lato (Brassicaceae). — Syst. Bot.19: 73–88.

    Google Scholar 

  • —,Franzke, A., Koch, M., 1997: Molecular phylogenetics ofThlaspi s. l. (Brassicaceae) based on chloroplast DNA restriction site variation and sequences of the internal transcribed spacer of nuclear ribosomal DNA. — Canad. J. Bot.75: 469–482.

    Google Scholar 

  • Pobedimova, E., 1969: Revisio generisCochlearia L., 1. — Novosti Sist. Vyssh. Rast.6: 67–106.

    Google Scholar 

  • —, 1970: Revisio generisCochlearia L., 2. — Novosti Sist. Vyssh. Rast7: 167–195.

    Google Scholar 

  • Prantl, K., 1891:Cruciferae. — InEngler, A., Prantl, K., (Eds): Die natürlichen Pflanzenfamilien,III 2, pp. 145–206. — Leipzig: Engelmann.

    Google Scholar 

  • Price, R. A., Al-Shehbaz, I. A., Palmer, J. D., 1994: Systematic relationships ofArabidopsis. A molecular and morphological perspective. — InMeyerowitz, E. M., Sommerville, C., (Eds):Arabidopsis, pp. 6–19. — New York: Cold Spring Harbour Press.

    Google Scholar 

  • Rathgeber, J., Capesius, I., 1989: Nucleotide sequence of the 18 S-25 S spacer region from mustard DNA. — Nucl. Acids Res.17: 7522.

    Google Scholar 

  • Schulz, O. E., 1923: Eine neue Sektion der GattungCochlearia L. SektionHilliella. — Notizbl. Bot. Gart. Berlin-Dahlem8: 544.

    Google Scholar 

  • —, 1933: SektionGlaucocochlearia. — Bot. Jahrb.66: 95.

    Google Scholar 

  • —, 1936:Cruciferae. — InEngler, A., Harms, H., (Eds): Die natürlichen Pflanzenfamilien2, 17 b, pp. 227–658. — Leipzig: Engelmann.

    Google Scholar 

  • Schultze-Motel, W., 1986:Cochlearia. — InConert, H. J., Hamann, U., Schultze-Motel, W., Wagenitz, G., (Eds):Gustav Hegi, Illustrierte Flora von Mitteleuropa. 3rd edn,IV/1, pp. 329–336. — Berlin: Parey.

    Google Scholar 

  • Swofford, D. L., 1993: PAUP: phylogenetic analysis using parsimon, version 3.1. A computer program distributed by the Illinois Natural History Survey, Champaign, Illinois.

  • Sytsma, K. J., 1990: DNA and morphology: inference of plant phylogeny. — Trends Ecol. Evol.5: 104–110.

    Google Scholar 

  • Taberlet, P., Gielly, L., Pautou, G., Bouvet, J., 1991: Universal primers for amplification of three non-coding regions of chloroplast DNA. — Pl. Molec. Biol.17: 1105–1109.

    Google Scholar 

  • Van de Peer, Y., De Wachter, R., 1994: Treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. — Computer Applic. Biosci.10: 569–570.

    Google Scholar 

  • Warwick, S. I., Black, L. D., 1991: Molecular systematics ofBrassica and allied genera (subtribeBrassicinae, Brassiceae) — chloroplast genome and cytodeme congruence. — Theor. Appl. Genet.82: 81–92.

    Google Scholar 

  • —, —,Aguinagalde, I., 1992: Molecular systematics ofBrassica and allied genera (subtribeBrassicinae, Brassiceae) — chloroplast DNA variation in the genusDiplotaxis. — Theor. Appl. Genet.83: 839–850.

    Google Scholar 

  • White, T. J., Bruns, T., Lee, S., Taylor, J., 1990: Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. — InInnis, M., (Ed.): PCR protocols: a guide to methods and applications, pp. 315–322. — San Diego: Academic Press.

    Google Scholar 

  • Wojciechowski, M. F., Sanderson, M. J., Baldwin, B. G., Donoghue, M. J., 1993: Monophyly of aneuploidAstragalus (Fabaceae): evidence from nuclear ribosomal DNA internal transcribed spacer sequences. — Amer. J. Bot.80: 711–722.

    Google Scholar 

  • Zhang, Y.-H., 1985:Cochleariopsis — a new genus of ChineseCruciferae. — Acta Bot. Yunnan.7: 143–145.

    Google Scholar 

  • —, 1986:Hilliella, a new genus ofCruciferae. — Acta Bot. Yunnan.8: 397–406.

    Google Scholar 

  • —, 1987a: A revision of genusYinshania (Cruciferae). — Acta Phyt. Sin.25: 204–218.

    Google Scholar 

  • —, 1987b: Five new species of theHilliella (Cruciferae). — Acta Bot. Yunnan.9: 153–161.

    Google Scholar 

  • —, 1993: A new species ofYinshania with a discussion of the evolution and origin of the genus. — Acta Bot. Yunnan.15: 364–368.

    Google Scholar 

  • —, 1995a: A new species ofHilliella (Cruciferae) from Anhui. — Acta Phytotax. Sin.33: 94–96.

    Google Scholar 

  • —, 1995b: On classification ofHilliella warburgii andCochleariella zhejiangensis. — Acta Phytotax. Sin.33: 175–178.

    Google Scholar 

  • —,Cai, J.-J., 1989: Observation on the generaYinshania, Hilliella, Cochleariella andCochlearia (Cruciferae) by SEM. — Acta Bot. Boreal.-Occid. Sin.9: 224–231.

    Google Scholar 

  • —,Xu, K., 1990: A numerical taxonomic study on two generaYinshania andHilliella (Cruciferae). — J. Wuhan Bot. Res.8: 317–324.

    Google Scholar 

  • Zunk, K., 1994: Chloroplasten-DNA-Restriktionsstellen-Analysen in der Familie derBrassicaceae. — Ph.D.-Thesis, University of Osnabrück, Osnabrück, Germany.

    Google Scholar 

  • —,Mummenhoff, K., Koch, M., Hurka, H., 1996: Phylogenetic relationships ofThlaspi s. l. (subtribeThlaspidinae, Lepideae) and allied genera based upon chloroplast DNA restriction-site variation. — Theor. Appl. Genet.92: 375–381.

    Google Scholar 

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Authors and Affiliations

  1. Max-Planck-Institut für Chemische Ökologie, Tatzendpromenade la, D-07745, Jena, Germany

    Marcus Koch

  2. Institute of Systematic Botany, University of Osnabrück, Barabarastraβe 11, D-49069, Osnabrück, Germany

    Klaus Mummenhoff & Herbert Hurka

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  1. Marcus Koch
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Koch, M., Mummenhoff, K. & Hurka, H. Molecular phylogenetics ofCochlearia (Brassicaceae) and allied genera based on nuclear ribosomal ITS DNA sequence analysis contradict traditional concepts of their evolutionary relationship. Pl Syst Evol 216, 207–230 (1999). https://doi.org/10.1007/BF01084399

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