Abstract
The aim of this work was to study in the adult rat heart the effect of modifications of fatty acid (FA) supply on the content of cytoplasmic fatty acid-binding protein (H-FABPc). To modify the amount of circulating lipids, three different treatments were chosen: (i) an hypolipidemic treatment with Clofibrate, administered daily through a gastric tube at a dose of 250 mg/kg per day for one week, (ii) a continuous intravenous infusion of 20% Intralipid, a fat emulsion, for one week at a dose of 96 ml/kg per day, and (iii) a normobaric hypoxia exposure (pO2=10%) for three weeks. At the end of each treatment plasma lipids, myocardial H-FABPc content and the activities of three key enzymes (citrate synthase, CS, fructrose-6-phosphate kinase, FPK and hydroxy-acyl CoA-dehydrogenase, HAD) were assessed. With each of the three treatments a decrease of plasma cholesterol and phospholipid levels was observed. Plasma FA concentration increased with Intralipid infusion and decreased with chronic hypoxia. The heart H-FABPc content was increased by 20% with Clofibrate, decreased by 20% with chronic hypoxia and remained unaltered upon Intralipid treatment. The induced changes in H-FABPc content were not related directly to changes in plasma lipid levels. CS activity was slightly decreased in the hypoxia group, FPK activity decreased in the Clofibrate group, and HAD activity decreased in the Intralipid group. Among the various groups heart H-FABPc content was related to HAD activity. In conclusion, the H-FABPc content of adult rat heart appears responsive to changes in plasma lipid levels.
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References
Van der Vusse GJ, Glatz JFC, Stam HCG, Reneman RS: Fatty acid homeostasis in the normoxic and ischemic heart. Physiol Rev 72:881–940, 1992
Randle PJ, Tubbs PK: Carbohydrate and fatty acid metabolism. In: Handbook of Physiology, section 2: Circulation, 1977, pp 805–844
Glatz JFC, Baerwaldt CCF, Veerkamp JH, Kempen HJM: Diurnal variation of cytosolic fatty acid-binding protein content and of palmitate oxidation in rat liver and heart. J Biol Chem 259:4295–4300, 1984
Bass NM: The cellular fatty acid binding proteins: aspects of structure, regulation and function. Int Rev Cytol 111:143–184, 1988
Matarese V, Stone RL, Waggoner DW, Bernlohr DA: Intracellular fatty acid trafficking and the role of cytosolic lipid binding proteins. Prog Lipid Res 28:245–272, 1989
Glatz JFC, Van der Vusse GJ: Cellular fatty acid-binding proteins: current concepts and future directions. Mol Cell Biochem 98:237–251, 1990
Veerkamp JH, Peeters RA, Maatman RGHI: Structural and functional features of different types of cytoplasmic fatty acid-binding proteins. Biochim Biophys Acta 1081:1–24, 1991
Cuchet P, Morrier C, Cand F, Keriel C: Effects of Clofibrate and Tiadenol on the elimination of lipids and bile acids in rat bile. Lipids 16:732–738, 1981
Vork MM, Glatz JFC, Surtel DAM, Knubben HJM, Van der Vusse GJ: A sandwich enzyme linked immuno-sorbent assay for the determination of rat heart fatty acid-binding protein using the streptavidin-biotin system. Application to tissue and effluent samples from normoxic rat heart perfusion. Biochim Biophys Acta 1075:199–205, 1991
Shepherd D, Garland PB: Citrate synthase from rat liver. Meth Enzymol 13:11–18, 1969
Ling KH, Paetkau V, Marous F, Lardy HA: Phosphofructokinase I. Skeletal muscle. Meth Enzymol 9:425–429, 1966
Bergmeyer HU: Methods of enzymatic analysis, Vol 1, pp 474–476. Academic Press, New York, 1974
Best NM, Duncan CH: Reduction of serum triglycerides and cholesterol by ethyl p-chlorophenoxyisobutyrate (CPIB). Amer J Cardiol 15:230–233, 1965
Best NM, Duncan CH: Effects of Clofibrate and dextrothyroxine singly and in combination on serum lipids. Arch Intern Med 118: 97–102, 1966
Breckenridge WC, Kakis G, Kuksis A: Identification of lipoprotein X-like particles in rat plasma following Intralipid perfusion. Can J Biochem 57:72–82, 1979
Lengle EE, Weeb L, Bokka E, Izzo R: Effect of lipid emulsion on cholesterol metabolism in dogs. Proc Fed Am Soc Exp Biol 40: 992A, 1981
Hulman G, Pearson HJ, Fraser I, Bell PRF: Agglutination of Intralipid by sera of acutely ill patients. Lancet ii: 1426–1428, 1982
Hulman G: Pathogenesis of non-traumatic fat embolism. Lancet i: 1366–1367, 1988
Banchero N: Cardiovascular responses to chronic hypoxia. Ann Rev Physiol 49:465–476, 1987
Pison C, Leverve X, Barnoud D, Pin I, Brichon PY, Blanc-Jouvan F, Paramelle B, Guignier M: Nutritional and metabolic status in 18 patients listed for lung transplantation. Ann Rev Resp Dis 145, 4, 304, 1992
Vork MM, Trigault N, Snoeckx LHEH, Glatz JFC, Van der Vusse GJ: Heterogenous distribution of fatty acid-binding protein in the hearts of Wistar Kyoto and Spontaneously Hypertensive rats. J Moll Cell Cardiol 24:317–321, 1992
Glatz JFC, Van der Vusse GJ, Veerkamp JH: Fatty acid-binding proteins and their physiological significance. News Physiol Sci 3: 41–43, 1988
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Garnier, A., Poizat, C., Keriel, C. et al. Modulation of fatty acid-binding protein content of adult rat heart in response to chronic changes in plasma lipid levels. Mol Cell Biochem 123, 107–112 (1993). https://doi.org/10.1007/BF01076481
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DOI: https://doi.org/10.1007/BF01076481