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Effects and relationship of intermittent hypoxia on serum lipid levels, hepatic low-density lipoprotein receptor-related protein 1, and hypoxia-inducible factor 1α

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Abstract

Objective

This study investigated the influence of intermittent hypoxia on serum lipid level, hepatic low-density lipoprotein receptor-related protein (LRP)1, and hepatic hypoxia-inducible factor (HIF)-1α and the underlying mechanisms of action.

Methods

Male Sprague Dawley rats were subjected to different levels of hypoxia. After 1–4 weeks hypoxemia, routine blood tests were performed and the levels of LRP1 and HIF-1α in liver were examined. Intermittent hypoxia (IH) was induced in HepG2 cells with or without HIF-1α inhibitor YC-1 pretreatment, and the levels of LRP1 and HIF-1α in cells were examined.

Results

IH caused elevated serum triglyceride, cholesterol, low-density lipoprotein, and high-density lipoprotein in rats. IH caused elevated hepatic levels of LRP1 and HIF-1α. After pretreatment with YC-1, HIF-1α protein expression decreased but mRNA expression did not change in HepG2 cells.

Conclusions

IH caused dyslipidemia and elevated LRP1 and HIF-1α. Elevated LRP1 expression was caused by HIF-1α.

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Abbreviations

LRP1:

Low-density lipoprotein receptor-related protein 1

HIF-1α:

Hypoxia- inducible factor 1α

IH:

Intermittent hypoxia

OSAS:

Obstructive sleep apnea syndrome

LDL:

Low-density lipoprotein

TG:

Total cholesterol

TC:

Triglyceride

VLDL:

Very low-density lipoprotein

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Conflicts of interest

The authors declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research reported.

Funding

This research did not receive any specific grant from any funding agency in the public, commercial, or not-for-profit sector.

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Authors and Affiliations

  1. Department of Respiratory Medicine, Shengjing Hospital, China Medical University, No. 36 Sanhao Street, Heping District, Shenyang, China

    Peng Li, Li Zhao & Xue-wei Feng

  2. Department of Anesthesiology, Shengjing Hospital, China Medical University, Shenyang, China

    Jian Wu

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  1. Peng Li
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Correspondence to Xue-wei Feng.

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Li, P., Wu, J., Zhao, L. et al. Effects and relationship of intermittent hypoxia on serum lipid levels, hepatic low-density lipoprotein receptor-related protein 1, and hypoxia-inducible factor 1α. Sleep Breath 20, 167–173 (2016). https://doi.org/10.1007/s11325-015-1200-4

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  • DOI: https://doi.org/10.1007/s11325-015-1200-4

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