Skip to main content
SpringerLink
Log in

Ileal epithelial cell migration in acute renal failure

Autoradiographic studies in the mouse

  • Original Articles
  • Published:
The American Journal of Digestive Diseases Aims and scope Submit manuscript

Abstract

Previous investigations have shown an inhibition of gastrointestinal epithelial cell proliferation in acutely uremic mice. This paper gives the results of an autoradiographic investigation with3H-thymidine of the effect of acute renal failure on ileal epithelial cell migration. Acute renal failure was produced by urinary outflow obstruction and control mice subjected to sham operation. The mean number of cell positions between the cryptvillus junction and the leading labeled cell 30 hours after operation was decreased in the uremic group (P<0.01). No significant difference was found in the number of cells per villus column. Estimates showed a decrease in mean migration rate from 0.69 to 0.55 positions per hour and an increase in the life span of villus cells from 54.2 to 73.8 hours. Decreased crypt and villus cell renewal may contribute to the development of uremic gastrointestinal lesions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Castrup HJ, Löhrs U, Eder M: Autoradiographische und histochemische Untersuchungen zur Entstehung der sogenannten urämischen Entercolitis (Autoradiographic and histochemical studies on the development of the socalled uremic entercolitis). Virchows Arch 349:357–367, 1970

    Google Scholar 

  2. Castrup HJ, Lennartz KJ: Anderung der Zellkinetik des oberflächenepithels des Magens bei Urämie. Autoradiographische Untersuchung mit der Prozent-markierte-mitosen-methode (Change in cell kinetics of gastric surface epithelium in uremia. Autoradiographic investigation with the percentage of labelled mitoses method). Beitr Pathol 146:315–321, 1972

    Google Scholar 

  3. McDermott FT, Galbraith AJ, Dalton MK: Effects of acute renal failure on ileal epithelial cell kinetics: autoradiographic studies in the mouse. Gastroenterology 66:235–239, 1974

    Google Scholar 

  4. McDermott FT, Dalton MK, Galbraith AJ: The effect of acute renal failure on mitotic duration of mouse ileal epithelium. Cell Tissue Kinet 7:31–36, 1974

    Google Scholar 

  5. McDermott FT, Nayman J, de Boer WGRM: Epithelial cell division in acute renal failure: a radio-autographic study in the oesophagus of the mouse. Br J Surg 58:52–55, 1971

    Google Scholar 

  6. McDermott FT, Nayman J, de Boer WGRM: The effect of acute renal failure upon cell division in the jejunum: radio-autographic and ultrastructural studies in the mouse. Ann Surg 174:274–282, 1971

    Google Scholar 

  7. McDermott FT, Nayman J, de Boer WGRM: The effect of acute renal failure upon wound healing: Histological and autoradiographic studies in the mouse. Ann Surg 168:142–146, 1968

    Google Scholar 

  8. Thrasher JD, Greulich RC: The duodenal progenitor population. II. Age related changes in size and distribution. J Exp Zool 159:385–396, 1965

    Google Scholar 

  9. Grad B, Stevens CE: Histological changes produced by single large injection of radioactive phosphorus (P32) in albino rats and in C3H mice. Cancer Res 10:289–296, 1950

    Google Scholar 

  10. Sherman FG, Quastler H: DNA synthesis in irradiated intestinal epithelium. Exp Cell Res 19:343–360, 1960

    Google Scholar 

  11. Altmann GG, Leblond CP: Factors influencing villus size in the small intestine of adult rats as revealed by transposition of intestinal segments. Am J Anat 127:15–36, 1970

    Google Scholar 

  12. Quastler H, Sherman FG: Cell population kinetics in the intestinal epithelium of the mouse. Exp Cell Res 17:420–438, 1959

    Google Scholar 

  13. Loehry CA, Creamer B: Three-dimensional structure of the human small intestinal mucosa in health and disease. Gut 10:6–12, 1969

    Google Scholar 

  14. Maisin JR, Lambiet M: Influence of mixture of chemical radioprotectors on the cellular renewal in the duodenum of mice. Nature 214:412–413, 1967

    Google Scholar 

  15. Leblond CP, Messier B: Renewal of chief cells and goblet cells in the small intestine as shown by radioautography after injection of thymidine-H3 into mice. Anat Rec 132:247–259, 1958

    Google Scholar 

  16. Fry RJM, Lesher S, Kohn HI: Age effect and cell transit time in mouse jejunal epithelium. Am J Physiol 201:213–216, 1961

    Google Scholar 

  17. Thrasher JD: Comparison of the cell cycle and cell migration in the intestinal epithelium of suckling and adult mice. Experientia 23:1050–1051, 1967

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Surgery, Monash University, Alfred Hospital, Melbourne, Australia

    Francis T. McDermott MB, FRACS, Melinda K. Dalton MB & Andrew J. Galbraith MB

Authors
  1. Francis T. McDermott MB, FRACS
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Melinda K. Dalton MB
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Andrew J. Galbraith MB
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This investigation was supported by the National Health and Medical Research Council.

Rights and permissions

Reprints and permissions

About this article

Cite this article

McDermott, F.T., Dalton, M.K. & Galbraith, A.J. Ileal epithelial cell migration in acute renal failure. Digest Dis Sci 19, 1116–1119 (1974). https://doi.org/10.1007/BF01076146

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01076146

Keywords

Search

Navigation