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Unique and selective mitogenic effects of vanadate on SV40-transformed cells

  • Part II: Biochemical and Physiological Studies
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Abstract

Vanadate and insulin both function as unique complete mitogens for SV40-transformed 3T3T cells, designated CSV3-1, but not for nontransformed 3T3T cells. The mitogenic effects induced by vanadate and insulin in CSV3-1 cells are mediated by different signaling mechanisms. For example, vanadate does not stimulate the tyrosine phosphorylation of the insulin receptor β-subunit nor the 170 kDa insulin receptor substrate-1. Instead, vanadate induces a marked increase in tyrosine phosphorylation of 55 and 64 kDa proteins that is not observed in insulin-stimulated CSV3-1 cells. Perhaps most interestingly, vanadate-induced mitogenesis is associated with the selective induction ofc-jun andjunB expression without significantly inducingc-fos orc-myc. Furthermore, treatment of CSV3-1 cells with genistein abolishes the effects of vanadate on protein tyrosine phosphorylation andc-jun induction. These and related data suggest that modulation of protein tyrosine phosphorylation andc-jun andjunB expression may serve the critical roles in mediating vanadate-induced mitogenesis in SV40-transformed cells.

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  1. Department of Pathology, The University of Tennessee College of Medicine, 899 Madison Avenue, 38163, Memphis, Tennessee, USA

    Hanlin Wang & Robert E. Scott

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Wang, H., Scott, R.E. Unique and selective mitogenic effects of vanadate on SV40-transformed cells. Mol Cell Biochem 153, 59–67 (1995). https://doi.org/10.1007/BF01075919

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