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The effect of prolactin and bombesin on the growth of meningioma-derived cells in monolayer culture

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Summary

Meningioma growth is thought to be stimulated by the sex hormones progesterone and possibly estrogen. We report here stimulation of growth of meningioma-derived cells in culture by prolactin.

Fourteen human tumors taken from surgery were initially grown in Ham's medium F10 with 15% fetal calf serum. The tumors were then trypsinized and resuspended in medium in a multi-well plate with either prolactin or bombesin; the cells were incubated for 1 week, washed, and resuspended for cell counting. The growth-stimulating effect of prolactin at 10 and 200 μg/ml was compared with bombesin at 5 mM/ml or 15 mM/ml. A growth index compared cell count in the experimental well to the control well; growth at the rate of the control well was given an index of 1.0.

The tumors included 7 meningiomas and 7 other neoplasms (3 astrocytomas, an ependymoma, a pine-oblastoma, a hemangiopericytoma, and a metastatic adenocarcinoma). For meningiomas incubated in 10 μg/ ml prolactin, the growth index was 3.08; for those incubated in 200 (μg/ml prolactin, it was 2.28. Bombesin indices were 1.7 and 1.2 at 5 mM/ml and 15 mM/ml, respectively. By 2-tailed t-testing both prolactin concentrations stimulated the growth of meningiomas significantly (P≤0.02), while bombesin did not. Neither peptide enhanced the growth of the other tumors tested.

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Authors and Affiliations

  1. Brain Tumor Center, Brigham and Women's Hospital, Children's Hospital, 02115, Boston, Massachusetts, USA

    Enrique Jimenez-Hakim, Mustapha El-Azouzi & Peter McL Black

  2. Dana-Farber Cancer Institute and The Neurosurgical Service, Brigham and Women's Hospital and Children's Hospital, 02115, Boston, Massachusetts, USA

    Enrique Jimenez-Hakim, Mustapha El-Azouzi & Peter McL Black

  3. Department of Surgery, Harvard Medical School, 02115, Boston, Massachusetts, USA

    Enrique Jimenez-Hakim, Mustapha El-Azouzi & Peter McL Black

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  1. Enrique Jimenez-Hakim
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  2. Mustapha El-Azouzi
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Jimenez-Hakim, E., El-Azouzi, M. & Black, P.M. The effect of prolactin and bombesin on the growth of meningioma-derived cells in monolayer culture. J Neuro-Oncol 16, 185–190 (1993). https://doi.org/10.1007/BF01057032

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