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Adhesion ofHelicobacter pylori strains toα-2,3-linked sialic acids

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Abstract

Helicobacter pylori is a human pathogen associated with gastritis and peptic ulcer. Adhesion properties ofH. pylori to various structures have been described in the literature, including evidence for sialic acid-binding. To study the specificity and frequency of sialic acid-binding, fourteenH. pylori strains were investigated using haemagglutination with derivatized erythrocytes carrying sialic acids only on defined glycans and using haemagglutination inhibition assays. From these studiesH. pylori strains can be grouped into sialic acid-dependent and sialic acid-independent classes. The sialic acid-dependent strains require α-2,3-linked sialic acid for haemagglutination. The potential roles of sialic acid-dependent adhesions forH. pylori-related infections are discussed.

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Abbreviations

Sia:

sialic acids

Neu5Ac:

N-acetyl-neuraminic acid

Neu5Gc:

N-glycolylneuraminic acid

Neu5Fm:

N-formylneuraminic acid

Neu5TFA:

N-trifluoroacetylneuraminic acid

RBC:

human red blood cells (erythrocytes)

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Authors and Affiliations

  1. Institute of Medical Microbiology, University of Lund, Sölvegatan 23, S-223 62, Lund, Sweden

    Siiri Hirmo & Torkel Wadström

  2. Biochemisches Institut, University of Kiel, Olshausenstr. 40, D-24098, Kiel, Germany

    Sørge Kelm & Roland Schauer

  3. Department of NBC Defence, National Defence Research Establishment, S-90182, Umeå, Sweden

    Bo Nilsson

Authors
  1. Siiri Hirmo
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  2. Sørge Kelm
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  3. Roland Schauer
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  4. Bo Nilsson
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  5. Torkel Wadström
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Hirmo, S., Kelm, S., Schauer, R. et al. Adhesion ofHelicobacter pylori strains toα-2,3-linked sialic acids. Glycoconjugate J 13, 1005–1011 (1996). https://doi.org/10.1007/BF01053196

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  • DOI: https://doi.org/10.1007/BF01053196

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