Abstract
Helicobacter pylori chronically infects the gastric mucosa of humans and diseases associated with infection include gastritis, peptic ulceration, and development of gastric cancer. The organism displays a distinct tropism for the gastric mucosa of humans and for the gastric mucin MUC5AC. While the majority of organisms are found in the mucus layer overlying the epithelial cells in the stomach, adherence of the organism to the gastric epithelium is necessary for the development of disease. The interaction of H. pylori with epithelial cells results in subversion of host cell signaling and induction of an inflammatory response. Factors that influence the outcome of infection include host genetics, environmental factors, and the phenotype of the infecting strain. In this chapter, we describe cell culture assays to assess the interaction of H. pylori with epithelial cells, immunofluorescent staining to detect H. pylori in infected human gastric biopsy specimens and the use of flow cytometry to detect mucin binding to H. pylori.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Schneider S, Carra G, Sahin U et al (2011) Complex cellular responses of Helicobacter pylori-colonized gastric adenocarcinoma cells. Infect Immun 79:2362–2371
Fiorentino M, Ding H, Blanchard TG et al (2013) Helicobacter pylori-induced disruption of monolayer permeability and proinflammatory cytokine secretion in polarized human gastric epithelial cells. Infect Immun 81:876–883
Tegtmeyer N, Wessler S, Necchi V et al (2017) Helicobacter pylori employs a unique basolateral type IV secretion mechanism for CagA delivery. Cell Host Microbe 22:552–560
Dolan B, Naughton J, Tegtmeyer N et al (2012) The interaction of Helicobacter pylori with the adherent mucus gel layer secreted by polarized HT29-MTX-E12 cells. PLoS One 7:e47300
Tan S, Noto JM, Romero-Gallo J et al (2011) Helicobacter pylori perturbs iron trafficking in the epithelium to grow on the cell surface. PLoS Pathog 7:e1002050
Drumm B, Sherman P (1989) Long-term storage of Campylobacter pylori. J Clin Microbiol 27:1655–1656
Dunne C, McDermot A, Anjan K et al (2017) Use of recombinant mucin glycoprotein to assess the interaction of the gastric pathogen Helicobacter pylori with the secreted human mucin MUC5AC. Bioengineering 4:E34
Lidell ME, Bara J, Hansson GC (2008) Mapping of the 45M1 epitope to the C-terminal cysteine-rich part of the human MUC5AC mucin. FEBS J 275:481–489
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2021 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Clyne, M., Dunne, C., Dolan, B. (2021). Investigating the Interaction of Helicobacter pylori with the Gastric Mucosa. In: Smith, S.M. (eds) Helicobacter Pylori. Methods in Molecular Biology, vol 2283. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1302-3_15
Download citation
DOI: https://doi.org/10.1007/978-1-0716-1302-3_15
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1301-6
Online ISBN: 978-1-0716-1302-3
eBook Packages: Springer Protocols