Skip to main content
Log in

Behavioral and electroantennogram analysis of olfactory stimulation inlozenge: ADrosophila mutant lacking antennal basiconic sensilla (Diptera: Drosophilidae)

  • Published:
Journal of Insect Behavior Aims and scope Submit manuscript

Abstract

Two alleles of the mutant lozengeof Drosophila melanogaster, lzand lz3,lack basiconic sensilla on the antennal funiculus. To elucidate the role of these sensilla for the perception of food odors, we studied the locomotor behavior and the electroantennogram (EAG) activity of lozenge flies in response to olfactory stimuli. The significance of basiconic sensilla on the maxillary palps was assessed by testing the locomotion of flies surgically deprived of their palps. The behavioral data suggest that antennal and maxillary basiconic sensilla may be important receptors for short chain alcohols and organic acids but less crucial receptors for acetates, aldehydes, and ketones. In agreement with this interpretation, EAG responses to alcohols (but not to esters) were found to be markedly lower in lozengethan in the wild type.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Altner, H., and Prillinger, L. (1980). Ultrastructure of invertebrate chemo-, thermo-, and hygroreceptors and its functional significance.Int. Rev. Cytol. 67: 69–139.

    Google Scholar 

  • Ayyub, C., Paranjape, J., Rodrigues, V., and Siddiqi, O. (1990). Genetics of olfactory behavior inDrosophila melanogaster.J. Neurogenet. 6: 243–262.

    PubMed  Google Scholar 

  • Benzer, S. (1967). Behavioral mutants ofDrosophila isolated by counter-current distribution.Proc. Natl. Acad. Sci. USA 58: 1112–1119.

    Google Scholar 

  • Boekhoff, I., Tareilus, E., Strotmann, J., and Breer, H. (1990). Rapid activation of alternative second messenger pathways in olfactory cilia from rats by different odorants.EMBO J. 9: 2453–2458.

    PubMed  Google Scholar 

  • Borst, A. (1984). Identification of different chemoreceptors by electroantennogram recording.J. Insect Physiol. 30: 507–510.

    Google Scholar 

  • Boulétrau, M., and Feuillet, P. (1981). An accurate and reliable olfactometer.Dros. Inf. Serv. 56: 172–174.

    Google Scholar 

  • Davies, J., Pirrotta, V., and Miklos, G. L. G. (1987). Analysis of theshaking B locus ofD. melanogaster.J. Neurogenet. 4: 123.

    Google Scholar 

  • Etcheto, M. (1980). A constant flow rate olfactometer with seven calibrated concentrations.Chem. Senses 5: 1–9.

    Google Scholar 

  • Fuyama, Y. (1976). Behavior genetics of olfactory responses inDrosophila. I. Olfactometry and strain differences inDrosophila melanogaster.Behav. Genet. 6: 407–420.

    PubMed  Google Scholar 

  • Lienhard, M. C., and Stocker, R. F. (1991). The development of the sensory neuron pattern in the antennal disc of wild-type and mutant (lz3, ssa)Drosophila melanogaster. Development112 (in press).

  • Link, B. (1983).Raster-und transmissionselektronenmikroskopische Analyse der Sensillen des mesothorakalen Beines und des dritten Antennensegmentes von Drosophila melanogaster, Diploma thesis, University of Fribourg, Fribourg, Switzerland.

    Google Scholar 

  • McKenna, M., Monte, P., Helfand, S. L., Woodard, C., and Carlson, J. (1989). A simple chemosensory response inDrosophila and the isolation ofacj mutations in which it is affected.Proc. Natl. Acad. Sci. USA 86: 8118–8122.

    PubMed  Google Scholar 

  • Rodrigues, V. (1980). Olfactory behavior ofDrosophila melanogaster. In Siddiqi, O., Babu, B., Hall, L., and Hall, J. C. (eds.), Plenum Press, New York, pp. 361–371.

    Google Scholar 

  • Rodrigues, V. (1988). Spatial coding of olfactory information in the antennal lobe ofDrosophila melanogaster.Brain Res. 453: 299–307.

    PubMed  Google Scholar 

  • Schaller, L. (1982). Structural and functional classification of antennal sensilla of the cockroach,Leucophaea maderae.Cell Tissue Res. 225: 129–142.

    PubMed  Google Scholar 

  • Siddiqi, O. (1984). Olfactory neurogenetics ofDrosophila. In Chopra, V. L., Joshi, B. C., Sharma, R. P., and Bawal, H. C. (eds.),Genetics: New Frontiers, Vol. III, Oxford University Press and IBH, London and New York, pp. 242–261.

    Google Scholar 

  • Siddiqi, O. (1987). Neurogenetics of olfaction inDrosophila melanogaster.Trends Genet. 3: 137–142.

    Google Scholar 

  • Singh, R. N., and Nayak S. V. (1985). Fine structure and primary sensory projections of sensilla on the maxillary palp ofDrosophila melanogaster Meigen (Diptera: Drosophilidae).Int. J. Insect Morphol. Embryol. 14: 291–306.

    Google Scholar 

  • Steinbrecht, R. A. (1987). Functional morphology of pheromone-sensitive sensilla. In Prestwich, G. D., and Blomquist, G. J. (eds.),Pheromone Biochemistry, Academic Press, New York, pp. 353–384.

    Google Scholar 

  • Stocker, R. F., and Gendre, N. (1988). Peripheral and central nervous effects oflozenge 3: ADrosophila mutant lacking basiconic antennal sensilla.Dev. Biol. 127: 12–24.

    PubMed  Google Scholar 

  • Stocker, R. F., and Gendre, N. (1989). Courtship behavior ofDrosophila, genetically or surgically deprived of basiconic sensilla.Behav. Genet. 19: 371–385.

    PubMed  Google Scholar 

  • Stocker, R. F., Singh, R. N., Schorderet, M., and Siddiqi, O. (1983). Projection patterns of different types of antennal sensilla in the antennal glomeruli ofDrosophila melanogaster.Cell Tissue Res. 232: 237–248.

    PubMed  Google Scholar 

  • Stocker, R. F., Lienhard, M. C., Borst, A., and Fischbach, K. F. (1990). Neuronal architecture of the antennal lobe inDrosophila melanogaster.Cell Tissue Res. 262:9–34.

    PubMed  Google Scholar 

  • Thomas, J. B., and Wyman, R. J. (1982). A mutation inDrosophila melanogaster alters normal connectivity between two identified neurons.Nature 298: 650–651.

    PubMed  Google Scholar 

  • Thomas, J. B., and Wyman, R. J. (1984). Mutations altering synaptic connectivity between identified neurons inDrosophila.J. Neurosci. 4: 530–538.

    PubMed  Google Scholar 

  • Venard, R., and Pichon, Y. (1981). Etude électroantennographique de la réponse périphérique de l'antenne deDrosophila melanogaster faite à l'aide de stimulations odorantes.C.R. Acad. Sci. Paris 293: 839–842.

    Google Scholar 

  • Venard, R., and Pichon, Y. (1984). Electrophysiological analysis of the peripheral response to odours in wild type and smell-deficientolf C mutant ofDrosophila melanogaster.J. Insect Physiol. 30: 1–5.

    Google Scholar 

  • Venard, R., Antony, C., and Jallon, J.-M. (1989).Drosophila chemoreceptors. In Singh, R. N., and Strausfeld, N. J. (eds.),Neurobiology of Sensory Systems, Plenum, New York and London, pp. 377–385.

    Google Scholar 

  • Venkatesh, S., and Singh, R. N. (1984). Sensilla on the third antennal segment ofDrosophila melanogaster Meigen (Diptera: Drosophilidae).Int. J. Insect Morphol. Embryol. 13: 51–63.

    Google Scholar 

  • Woodard, C., Huang, T., Sun, H., Helfand, S. L., and Carlson, (1989). Genetic analysis of olfactory behavior in Drosophila — A new screen yields the mutants.Genetics 123: 315–326.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Venard, R., Stocker, R.F. Behavioral and electroantennogram analysis of olfactory stimulation inlozenge: ADrosophila mutant lacking antennal basiconic sensilla (Diptera: Drosophilidae). J Insect Behav 4, 683–705 (1991). https://doi.org/10.1007/BF01052225

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01052225

Key words

Navigation