Skip to main content
SpringerLink
Log in

The first normal oral mucosa epithelium in which γ-glutamyl transpeptidase activity has been detected

  • Papers
  • Published:
The Histochemical Journal Aims and scope Submit manuscript

Summary

A portion of consistently γ-glutamyl transpeptidase-positive epithelium in the normal oral mucosa of rats is described. This is the first normal oral mucosa epithelium reported to express activity of the transpeptidase. This enzyme has been used as a marker of malignant transformation in tissues, such as epidermis and oral mucosa epithelium. Complementary studies of the enzyme-positive portion of oral mucosa and a neighbouring negative portion, suggest that, in this model, expression of γ-glutamyl transpeptidase is linked to a terminally-differentiated epithelium.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Adjarov, D., Ivanov, E., Petrova, S., Savov, G., Mirkova, E. &Keremidchiev, D. (1979) Developmental studies on gamma-glutamyl transferase in rat intestinal mucosa.Enzyme 24, 358–61.

    PubMed  Google Scholar 

  • Ahlund-Lindquist, C. &Lindskog, S. (1985) Activity of gamma-glutamyl transferase in developing teeth of the rat.Scand. J. Dent. Res. 93, 421–5.

    PubMed  Google Scholar 

  • Albert, Z., Rzucidlo, Z. &Starzyk, H. (1970) Comparative biochemical and histochemical studies on the activity of gamma-glutamyl transpeptidase in the organs of fetuses, newborns and adult rats.Acta Histochem. 34, 37–9.

    Google Scholar 

  • Buxman, M. M., Kocarnik, M. J. &Holbrook, K. A. (1979) Differentiation markers in fetal epidermis: transglutaminase and transpeptidase.J. Invest. Dermatol. 72, 171–7.

    PubMed  Google Scholar 

  • Cabrini, R. L., Schwint, A. E., Mendez, A., Femopase, F., Lanfranchi, H. & Itoiz, M. E. (1992) A morphometric study of nucleolar organizer regions in human oral normal mucosa, papilloma and squamous cell carcinoma.J. Oral Pathol. Med. (in press).

  • Calderon-Solt, L. &Solt, D. B. (1985) Gamma-glutamyl transpeptidase in precancerous lesions and carcinomas of oral, pharyngeal and laryngeal mucosa.,Cancer 56, 138–43.

    PubMed  Google Scholar 

  • Cameron, R., Kellen, J., Kolin, A., Malkin, A. &Farber, E. (1978) Gamma-glutamyltransferase in putative premalignant liver cell populations during hepatocarcinogenesis.Cancer Res. 38, 823–9.

    PubMed  Google Scholar 

  • De Young, L. M., Richards, W. L., Bonzelet, W., Tsai, L. L. &Boutwell, R. K. (1978) Localization and significance of gamma-glutamyl transpeptidase in normal and neoplastic mouse skin.Cancer Res. 38, 3697–701.

    PubMed  Google Scholar 

  • Fiala, S. &Fiala, E. S. (1973) Activation by chemical carcinogens of gamma-glutamyl transpeptidase in rat and mouse liver.J. Natl Cancer Inst. 51, 151–8.

    PubMed  Google Scholar 

  • Fiala, S., Mohindru, A., Kettering, W. G., Fiala, A. E. &Morris, H. P. (1976) Glutathione and gamma-glutamyl transpeptidase in rat liver during chemical carcinogenesis.JNCI 57, 591–8.

    PubMed  Google Scholar 

  • Fiala, S., Fiala, A. E., Keller, R. W. &Fiala, E. S. (1977) Gammaglutamyltranspeptidase in colon cancer induced by 1,2-dimethylhydrazine.Arch. Gesch. Wulstforsch. 47, 117–22.

    Google Scholar 

  • Fiala, S., Trout, E., Pragani, B., &Fiala, E. S. (1979) Increased gamma-glutamyltransferase activity in human colon cancer.Lancet i, 1145.

    Google Scholar 

  • Fiala, S., Trout, E. C., Teague, C. A. &Fiala, E. S. (1980) Gamma-glutamyl transferase, a common marker of human epithelial tumors?Cancer Detect. Prev. 3, 471–85.

    PubMed  Google Scholar 

  • Fraser, F. J. (1982) A selective stain for mitotic figures, particularly in the developing brain.Stain Technol. 57, 219–24.

    PubMed  Google Scholar 

  • Griffith, O. W., Bridges R. J. &Meister, A. (1979) Transport of gamma-glutamyl amino acids: role of glutathione and gamma-glutamyl transpeptidase.Proc. Natl Acad.Sci. USA 76, 6319–22.

    PubMed  Google Scholar 

  • Howat, A. J., Dilip, D. Giri, Cotton, D. W. K. &Slater, D. N. (1989) Nucleolar organizer regions in spitz nevi and malignant melanomas.Cancer 63, 474–8.

    PubMed  Google Scholar 

  • Klein-Szanto, A. J. P., Nelson, K. G., Shah, Y. &Slaga, T. J. (1983) Simultaneous appearance of keratin modifications and gamma-glutamyl transferase activity as indicators of tumor progression in mouse skin papillomasJNCI 70, 161–8.

    PubMed  Google Scholar 

  • Marathe, G. V., Nash, B., Haschemeyer, R. H. &Tate, S. S. (1979) Ultrastructural localization of gamma-glutamyl transpeptidase in rat kidney and jejunum.FEBS Lett. 107, 436–40.

    PubMed  Google Scholar 

  • Mock, D., Whitestone, B. &Freeman, J. (1987) Gamma-glutamyl transpeptidase activity in human oral squamous cell carcinoma.Oral Surg. Oral Med. Oral Pathol. 64, 197–201.

    PubMed  Google Scholar 

  • Novogrodsky, A., Tate, S. S. &Meister, A. (1976) Gammaglutamyl transpeptidase, a lymphoid cell surface marker: relationship to blastogenesis, differentiation and neoplasia.Proc. Natl Acad. Sci. USA 73, 2414–8.

    PubMed  Google Scholar 

  • Ploton, D., Menager, M., Jeannesson, P., Himber, G., Pigeon, F. &Adnet, J. J. (1986) Improvement in the staining and in the visualization of the argyrophilic proteins of the nucleolar organizer region at the optical level.Histochem. J. 18, 5–14.

    PubMed  Google Scholar 

  • Reibel, J., Clausen, H. &Dabelsteen, E. (1985) Staining patterns of human pre-malignant oral epithelium and squamous cell carcinomas by monoclonal anti-keratin antibodies.Acta Path. Microb. Immunol. Scand. Sect. A 93, 323–30.

    Google Scholar 

  • Richards, W. L. &Astrup, E. G. (1982) Expression of gamma-glutamyl transpeptidase activity in the developing mouse tooth, intervertebral disc, and hair follicle.Cancer Res. 42, 4143–52.

    PubMed  Google Scholar 

  • Rosalki, S. B. (1975) Gamma-glutamyl transpeptidase.Adv. Clin. Chem. 17, 53–107.

    PubMed  Google Scholar 

  • Rutenburg, A. M., Kim, H., Fischbein, J. W., Hanker, J. S., Wasserkrug, H. L. &Seligman, A. M. (1969) Histochemical and ultrastructural demonstration of gammaglutamyl transpeptidase activity.J. Histochem. Cytochem. 17, 517–26.

    PubMed  Google Scholar 

  • Schwint, A. E., Mendez, A. E., Itoiz, M. E. &Cabrini, R. L. (1990) A quantitative method for the measurement of parameters related to nucleolar organizer regions (NOR) on microphotographs: exemplification using rat oral mucosa.J. Dent. Res. 69, 920 (abstract).

    Google Scholar 

  • Solt, D. B. (1981) Localization of gamma-glutamyl transpeptidase in hamster buccal pouch epithelium treated with7,12-dimethylbenz[a]anthracene.JNCI,67, 193–200.

    PubMed  Google Scholar 

  • Solt, D. B. &Shklar, G. (1982) Rapid induction of gamma-glutamyl transpeptidase-rich intraepithelial clones in7,12-dimethylbenz[a]anthracene-treated hamster buccal pouch.Cancer Res. 42, 285–91.

    PubMed  Google Scholar 

  • Sternberg, L. A., Hardy, P. H., Cuculis, J. J. &Meyer, H. G. (1970) The unlabeled antibody enzyme method of immunohistochemistry. Preparation and properties of soluble antigenantibody complex (horseradish peroxidase) and its use in identification of spirochetes.J. Histochem. Cytochem. 18, 315–33.

    PubMed  Google Scholar 

  • Vanderlaan, M. &Phares, W. (1981) Gamma-glutamyl transpeptidase: a tumor cell marker with a pharmacological function (review).Histochem. J. 13, 865–77.

    PubMed  Google Scholar 

  • Vanderlaan, M., Cutter, C. &Dolbeare, F., (1979) Flow microfluorometric identification of liver cells with elevated gamma-glutamyl transpeptidase activity after carcinogen exposure.J. Histochem. Cytochem. 27, 114–9.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Radiobiology, National Atomic Energy Commission, Av. del Libertador 8250, (1429), Buenos Aires, Argentina

    A. E. Schwint, R. L. Cabrini & M. E. Itoiz

  2. Department of Oral Pathology, Faculty of Dentistry, University of Buenos Aires, M. T. de Alvear, (1122), Buenos Aires, Argentina

    A. M. Collet, A. E. Mendez, R. L. Cabrini & M. E. Itoiz

Authors
  1. A. E. Schwint
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. M. Collet
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. E. Mendez
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. L. Cabrini
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. E. Itoiz
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Schwint, A.E., Collet, A.M., Mendez, A.E. et al. The first normal oral mucosa epithelium in which γ-glutamyl transpeptidase activity has been detected. Histochem J 24, 964–968 (1992). https://doi.org/10.1007/BF01046502

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01046502

Keywords

Search

Navigation